{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:12:41Z","timestamp":1777983161599,"version":"3.51.4"},"reference-count":65,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T00:00:00Z","timestamp":1663200000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Instituto Nacional de Ci\u00eancia e Tecnologia em Entomologia Molecular, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil","award":["E-18\/2015"],"award-info":[{"award-number":["E-18\/2015"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq), Instituto Nacional de Ci\u00eancia e Tecnologia em Entomologia Molecular, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil","award":["221797"],"award-info":[{"award-number":["221797"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["E-18\/2015"],"award-info":[{"award-number":["E-18\/2015"]}]},{"name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro (FAPERJ)","award":["221797"],"award-info":[{"award-number":["221797"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Zika virus (ZIKV) is a positive-sense single-stranded RNA flavivirus and is mainly transmitted by Aedes mosquitoes. This arbovirus has had a significant impact on health in recent years by causing malformations, such as microcephaly in babies and Guillain\u2013Barr\u00e9 syndrome in adults. Some evidence indicates that ZIKV can be sexually transmitted and may persist in the male reproductive tract for an extended period in humans. Knockout and vasectomized mice have been used as models to reveal ZIKV infection in the male reproductive tract as a virus source. ZIKV presence in male and female mosquito reproductive tracts and eggs point to venereal and vertical\/transovarian transmission, again demonstrating that the reproductive tract can be involved in the spread of ZIKV. Moreover, eggs protected by eggshells have the potential to be a ZIKV reservoir. Given the +-lack of vaccines and therapies for Zika fever and the underestimated prevalence rate, an understanding of ZIKV infection and its spread from the reproductive tract, which is protected from the immune system and potentially active for virus transmission, is imperative. We must also develop cheaper, more efficient techniques for virological surveillance inside vectors and humans, control vectors with ecofriendly insecticides, and promote condom use to avoid ZIKV contamination during sexual intercourse, as recommended by the World Health Organization.<\/jats:p>","DOI":"10.3390\/v14092047","type":"journal-article","created":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T03:48:04Z","timestamp":1663300084000},"page":"2047","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evidence of Spreading Zika Virus Infection Caused by Males of Different Species"],"prefix":"10.3390","volume":"14","author":[{"given":"Thayane da Encarna\u00e7\u00e3o","family":"S\u00e1-Guimar\u00e3es","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3720-415X","authenticated-orcid":false,"given":"Monica Ferreira","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika Virus (I). Isolations and serological specificity","volume":"46","author":"Dick","year":"1952","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_2","unstructured":"WHO\u2014World Health Organization (2020, May 05). Fact Sheets, Detail, Zika Virus. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/zika-virus."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.jaut.2016.02.006","article-title":"The Zika outbreak of the 21st century","volume":"68","author":"Chang","year":"2016","journal-title":"J. Autoimmun."},{"key":"ref_4","unstructured":"WHO\u2014World Health Organization (2020, May 24). Prevention of Sexual Transmission of Zika Virus: Interim Guidance Update. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/204421."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.5123\/S1679-49742017000100002","article-title":"S\u00edndrome de Guillain-Barr\u00e9 e outras manifesta\u00e7\u00f5es neurol\u00f3gicas possivelmente relacionadas \u00e0 infec\u00e7\u00e3o pelo v\u00edrus Zika em munic\u00edpios da Bahia, 2015","volume":"26","author":"Malta","year":"2017","journal-title":"Pidemiologia E Servi\u00e7os De Sa\u00fade"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"675","DOI":"10.2471\/BLT.16.171082","article-title":"Zika: The origin and spread of a mosquito-borne virus","volume":"94","author":"Kindhauser","year":"2016","journal-title":"Bull. World Health Organ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"67","DOI":"10.4103\/npmj.npmj_36_18","article-title":"Pathogenesis, diagnostic challenges and treatment of zika virus disease in resource-limited settings","volume":"25","author":"Shehu","year":"2018","journal-title":"Niger. Postgrad. Med. J."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2536","DOI":"10.1056\/NEJMoa0805715","article-title":"Zika Virus Outbreak on Yap Island, Federated States of Micronesia","volume":"360","author":"Duffy","year":"2009","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3201\/eid1705.101939","article-title":"Probable non\u2013vector-borne transmission of Zika virus, Colorado, USA","volume":"17","author":"Foy","year":"2011","journal-title":"Emerg. Infect Dis."},{"key":"ref_10","first-page":"e64","article-title":"Potential effect of Zika virus infection on human male fertility? Avelino-Silva, Vivian Iida, et al. Potential effect of Zika virus infection on human male fertility?","volume":"60","author":"Alvarenga","year":"2018","journal-title":"Rev. Do Inst. De Med. Trop. De S\u00e3o Paulo"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"30148","DOI":"10.2807\/1560-7917.ES.2016.21.8.30148","article-title":"An autochthonous case of Zika due to possible sexual transmission, Florence, Italy, 2014","volume":"21","author":"Venturi","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"30252","DOI":"10.2807\/1560-7917.ES.2016.21.23.30252","article-title":"Sexual transmission of Zika virus in Germany, April 2016","volume":"21","author":"Frank","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.jcv.2017.10.006","article-title":"Persistence and infectivity of Zika virus in semen after returning from endemic areas: Report of 5 cases","volume":"96","author":"Arribas","year":"2017","journal-title":"J. Clin. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2195","DOI":"10.1056\/NEJMc1604449","article-title":"Evidence of Sexual Transmission of Zika Virus","volume":"374","author":"Matheron","year":"2016","journal-title":"N. Engl. J. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1016\/S0140-6736(16)30775-9","article-title":"Late sexual transmission of Zika virus related to persistence in the semen","volume":"387","author":"Turmel","year":"2016","journal-title":"Lancet"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1855","DOI":"10.3201\/eid2210.160951","article-title":"Sexual Transmission of Zika Virus and Persistence in Semen, New Zealand, 2016","volume":"22","author":"Harrower","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"215","DOI":"10.15585\/mmwr.mm6508e2","article-title":"Transmission of Zika Virus Through Sexual Contact with Travelers to Areas of Ongoing Transmission\u2014Continental United States, 2016","volume":"65","author":"Hills","year":"2016","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.eimc.2016.10.009","article-title":"Persistence of Zika virus in semen 93 days after the onset of symptoms","volume":"36","author":"Souto","year":"2018","journal-title":"Enferm. Infecc. Y Microbiol. Clin."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1093\/cid\/ciw692","article-title":"Male-to-Female Sexual Transmission of Zika Virus\u2014United States, January\u2013April 2016","volume":"64","author":"Russell","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"716","DOI":"10.15585\/mmwr.mm6528e2","article-title":"Suspected Female-to-Male Sexual Transmission of Zika Virus\u2014New York City, 2016","volume":"65","author":"Davidson","year":"2016","journal-title":"MMWR. Morb. Mortal. Wkly. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/S1473-3099(17)30444-9","article-title":"Effect of acute Zika virus infection on sperm and virus clearance in body fluids: A prospective observational study","volume":"17","author":"Joguet","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1001.e1","DOI":"10.1016\/j.cmi.2017.07.006","article-title":"Detection of Zika virus RNA in semen of asymptomatic blood donors","volume":"23","author":"Musso","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1016\/S1473-3099(16)30320-6","article-title":"Probable sexual transmission of Zika virus from a vasectomised man","volume":"16","author":"Arsuaga","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2217\/fvl-2019-0159","article-title":"The impact of Zika virus infection on human semen: A case study","volume":"15","author":"Azevedo","year":"2020","journal-title":"Futur. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"137","DOI":"10.3201\/eid2301.161300","article-title":"Persistent Zika Virus Detection in Semen in a Traveler Returning to the United Kingdom from Brazil, 2016","volume":"23","author":"Gaskell","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"30314","DOI":"10.2807\/1560-7917.ES.2016.21.32.30314","article-title":"Persistent detection of Zika virus RNA in semen for six months after symptom onset in a traveller returning from Haiti to Italy, February 2016","volume":"21","author":"Nicastri","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Oliveira, D.B.L., Durigon, G.S., Mendes, \u00c9.A., Ladner, J.T., Andreata-Santos, R., Araujo, D.B., Botosso, V.F., Paola, N.D., Neto, D.F.L., and Cunha, M.P. (2018). Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses, 10.","DOI":"10.3390\/v10110615"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1093\/cid\/cix967","article-title":"Virus and Antibody Dynamics in Travelers with Acute Zika Virus Infection","volume":"66","author":"Barzon","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Froeschl, G., Huber, K., Von Sonnenburg, F., Nothdurft, H.-D., Bretzel, G., Hoelscher, M., Zoeller, L., Trottmann, M., Pan-Montojo, F., and Dobler, G. (2017). Long-term kinetics of Zika virus RNA and antibodies in body fluids of a vasectomized traveller returning from Martinique: A case report. BMC Infect. Dis., 17.","DOI":"10.1186\/s12879-016-2123-9"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1056\/NEJMoa1711038","article-title":"Zika Virus Shedding in Semen of Symptomatic Infected Men","volume":"378","author":"Mead","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1598","DOI":"10.3201\/eid2508.181543","article-title":"Prolonged Zika Virus RNA Detection in Semen of Immunosuppressed Patient","volume":"25","author":"Petridou","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"611","DOI":"10.3201\/eid2304.161692","article-title":"Presence and Persistence of Zika Virus RNA in Semen, United Kingdom, 2016","volume":"23","author":"Atkinson","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1056\/NEJMoa1613108","article-title":"Persistence of Zika Virus in Body Fluids\u2014Final Report","volume":"379","author":"Rosenberg","year":"2018","journal-title":"New Engl. J. Med."},{"key":"ref_34","first-page":"31","article-title":"Duration of the Presence of Infectious Zika Virus in Semen and Serum","volume":"219","author":"Medina","year":"2018","journal-title":"J. Infect. Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Counotte, M.J., Kim, C.R., Wang, J., Bernstein, K., Deal, C.D., Broutet, N.J.N., and Low, N. (2018). Sexual transmission of Zika virus and other flaviviruses: A living systematic review. PLOS Med., 15.","DOI":"10.1371\/journal.pmed.1002611"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S1473-3099(19)30678-4","article-title":"1 year after acute Zika virus infection in men","volume":"20","author":"Bujan","year":"2020","journal-title":"Lancet Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"808","DOI":"10.3201\/eid2504.180977","article-title":"Prolonged Shedding of Zika Virus RNA in Vaginal Secretions, Nicaragua","volume":"25","author":"Reyes","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_38","first-page":"92","article-title":"Zika virus dynamics in body fluids and risk of sexual transmission in a non-endemic area","volume":"23","author":"Pou","year":"2017","journal-title":"Trop. Med. Int. Health"},{"key":"ref_39","first-page":"30","article-title":"Persistence of ZIKV-RNA in the cellular fraction of semen is ac-companied by a surrogate-marker of viral replication. Diagnostic implications for sexual transmission","volume":"41","author":"Biava","year":"2018","journal-title":"New Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1007\/s10815-019-01493-y","article-title":"An update on the aspects of Zika virus infection on male reproductive system","volume":"36","author":"Borges","year":"2019","journal-title":"J. Assist. Reprod. Genet."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1016\/j.celrep.2017.01.056","article-title":"Frequent Zika Virus Sexual Transmission and Prolonged Viral RNA Shedding in an Immunodeficient Mouse Model","volume":"18","author":"Duggal","year":"2017","journal-title":"Cell Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"15379","DOI":"10.1038\/s41598-018-33528-2","article-title":"Coitus-Free Sexual Transmission of Zika Virus in a Mouse Model","volume":"8","author":"Clancy","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1038\/nature20556","article-title":"Zika virus infection damages the testes in mice","volume":"540","author":"Govero","year":"2016","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"McDonald, E.M., Duggal, N.K., Ritter, J.M., and Brault, A.C. (2018). Infection of epididymal epithelial cells and leukocytes drives seminal shedding of Zika virus in a mouse model. PLOS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006691"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"McDonald, E.M., Duggal, N.K., and Brault, A.C. (2017). Pathogenesis and sexual transmission of Spondweni and Zika viruses. PLOS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0005990"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"4510","DOI":"10.1038\/s41598-018-22840-6","article-title":"Sexual transmission of Zika virus enhances in utero transmission in a mouse model","volume":"8","author":"Duggal","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1016\/j.virol.2019.02.004","article-title":"Mutations present in a low-passage Zika virus isolate result in attenuated pathogenesis in mice","volume":"530","author":"Duggal","year":"2019","journal-title":"Virology"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virol.2019.06.010","article-title":"Duration of seminal Zika viral RNA shedding in immunocompetent mice inoculated with Asian and African genotype viruses","volume":"535","author":"McDonald","year":"2019","journal-title":"Virology"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Fulton, B.O., Sachs, D., Schwarz, M.C., Palese, P., and Evans, M.J. (2017). Transposon Mutagenesis of the Zika Virus Genome Highlights Regions Essential for RNA Replication and Restricted for Immune Evasion. J. Virol., 91.","DOI":"10.1128\/JVI.00698-17"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"802","DOI":"10.2471\/BLT.17.181370","article-title":"Zika virus in semen: A prospective cohort study of symptomatic travellers returning to Belgium","volume":"95","author":"Huits","year":"2017","journal-title":"Bull. World Health Organ."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1242\/jcs.14.1.51","article-title":"Structural and Functional Modifications of the Nucleus during Oogenesis in the Mosquito Aedes Aegypti","volume":"14","author":"Fiil","year":"1974","journal-title":"J. Cell Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1016\/j.ibmb.2007.07.017","article-title":"A chitin-like component in Aedes aegypti eggshells, eggs and ovaries","volume":"37","author":"Moreira","year":"2007","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.jinsphys.2015.10.006","article-title":"Physical features and chitin content of eggs from the mosquito vectors Aedes aegypti, Anopheles aquasalis and Culex quinquefasciatus: Connection with distinct levels of resistance to desiccation","volume":"83","author":"Farnesi","year":"2015","journal-title":"J. Insect Physiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"S\u00e1-Guimar\u00e3es, T.D.E., Salles, T.S., dos Santos, C.R., Moreira, M.F., de Souza, W., and Caldas, L.A. (2021). Route of Zika virus infection in Aedes aegypti by transmission electron microscopy. BMC Microbiol., 21.","DOI":"10.1186\/s12866-021-02366-0"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"880","DOI":"10.3201\/eid2305.162041","article-title":"Vertical Transmission of Zika Virus by Aedes aegypti and Ae. Albopictus Mosquitoes","volume":"23","author":"Ciota","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_56","first-page":"1","article-title":"Vector competence and transovarial transmission of two Aedes aegypti strains to Zika virus","volume":"6","author":"Li","year":"2017","journal-title":"Emerg. Microbes Infect."},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Huang, Y.-J.S., Lyons, A.C., Hsu, W.-W., Park, S.L., Higgs, S., and VanLandingham, D.L. (2017). Differential outcomes of Zika virus infection in Aedes aegypti orally challenged with infectious blood meals and infectious protein meals. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0182386"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"e180290","DOI":"10.1590\/0074-02760180290","article-title":"Zika infection decreases Aedes aegypti locomotor activity but does not influence egg production or viability","volume":"113","author":"Padilha","year":"2018","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1590\/0074-02760160332","article-title":"First detection of natural infection of Aedes aegypti with Zika virus in Brazil and throughout South America","volume":"111","author":"Ribeiro","year":"2016","journal-title":"Mem\u00f3rias do Instituto Oswaldo Cruz"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"104434","DOI":"10.1016\/j.meegid.2020.104434","article-title":"Surveillance of Zika virus in field-caught Aedes aegypti and Aedes albopictus suggests important role of male mosquitoes in viral populations maintenance in Medell\u00edn, Colombia","volume":"85","author":"Rojo","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1186\/s13071-017-2543-4","article-title":"Zika virus can be venereally transmitted between Aedes aegypti mosquitoes","volume":"10","author":"Campos","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1590\/0074-02760170329","article-title":"First evidence of Zika virus venereal transmission in Aedes aegypti mosquitoes","volume":"113","author":"Nascimento","year":"2017","journal-title":"Mem\u00f3rias Do Inst. Oswaldo Cruz"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3011","DOI":"10.3389\/fmicb.2018.03011","article-title":"Zika Virus Infection Produces a Reduction on Aedes aegypti Lifespan but No Effects on Mosquito Fecundity and Oviposition Success","volume":"9","author":"Petersen","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_64","unstructured":"CDC\u2014Centers for Disease Control and Prevention (2020, May 30). Sexual Transmission and Prevention, Available online: https:\/\/www.cdc.gov\/zika\/prevention\/sexual-transmission-prevention.html."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"ciw509","DOI":"10.1093\/cid\/ciw509","article-title":"Long-Lasting Persistence of Zika Virus in Semen","volume":"63","author":"Matheron","year":"2016","journal-title":"Clin. Infect. Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/2047\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:32:12Z","timestamp":1760142732000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/9\/2047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,15]]},"references-count":65,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["v14092047"],"URL":"https:\/\/doi.org\/10.3390\/v14092047","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,15]]}}}