{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T13:44:41Z","timestamp":1789047881404,"version":"build-2803163510"},"reference-count":56,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["32102842"],"award-info":[{"award-number":["32102842"]}]},{"name":"National Natural Science Foundation of China","award":["BE2021369"],"award-info":[{"award-number":["BE2021369"]}]},{"name":"Key Research and Development Plan of Jiangsu Province","award":["32102842"],"award-info":[{"award-number":["32102842"]}]},{"name":"Key Research and Development Plan of Jiangsu Province","award":["BE2021369"],"award-info":[{"award-number":["BE2021369"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Cyprinid herpesvirus 2 (CyHV-2) is a causative factor of herpesviral hematopoietic necrosis (HVHN) in farmed crucian carp (Carassius carassius) and goldfish (Carassius auratus). In this study, we analyzed the genomic characteristics of a new strain, CyHV-2 SH-01, isolated during outbreaks in crucian carp at a local fish farm near Shanghai, China. CyHV-2 SH-01 exhibited a high sensitivity to goldfish and crucian carp in our previous research. The complete genome of SH-01 is 290,428 bp with 154 potential open reading frames (ORFs) and terminal repeat (TR) regions at both ends. Compared to the sequenced genomes of other CyHVs, Carassius auratus herpesvirus (CaHV) and Anguillid herpesvirus 1 (AngHV-1), several variations were found in SH-01, including nucleotide mutations, deletions, and insertions, as well as gene duplications, rearrangements, and horizontal transfers. Overall, the genome of SH-01 shares 99.60% of its identity with that of ST-J1. Genomic collinearity analysis showed that SH-01 has a high degree of collinearity with another three CyHV-2 isolates, and it is generally closely related to CaHV, CyHV-1, and CyHV-3, although it contains many differences in locally collinear blocks (LCBs). The lowest degree of collinearity was found with AngHV-1, despite some homologous LCBs, indicating that they are evolutionarily the most distantly related. The results provide new clues to better understand the CyHV-2 genome through sequencing and sequence mining.<\/jats:p>","DOI":"10.3390\/v14092068","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:08:09Z","timestamp":1663718889000},"page":"2068","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Complete Genome and Molecular Characterization of a New Cyprinid Herpesvirus 2 (CyHV-2) SH-01 Strain Isolated from Cultured Crucian Carp"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2072-2898","authenticated-orcid":false,"given":"Jia","family":"Yang","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxuan","family":"Wen","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simin","family":"Xiao","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Ocean Technology, College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224051, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7735-0960","authenticated-orcid":false,"given":"Fei","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Marine Biology, College of Oceanography, Hohai University, Nanjing 210098, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patarida","family":"Roengjit","sequence":"additional","affiliation":[{"name":"Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket 83000, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqun","family":"Lu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8893-1568","authenticated-orcid":false,"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China"},{"name":"National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1111\/j.1365-2761.1995.tb00296.x","article-title":"Herpesviral haematopoietic necrosis of goldfish, Carassius auratus (L.)","volume":"18","author":"Jung","year":"1995","journal-title":"J. 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