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Paris","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"International Affairs Department of the Institut Pasteur of Paris","award":["AFRIPOX"],"award-info":[{"award-number":["AFRIPOX"]}]},{"name":"Agence National de la Recherche","award":["2021YFC0863400"],"award-info":[{"award-number":["2021YFC0863400"]}]},{"name":"Agence National de la Recherche","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"Agence National de la Recherche","award":["ANSO-CR-SP-2020-02"],"award-info":[{"award-number":["ANSO-CR-SP-2020-02"]}]},{"name":"Agence National de la Recherche","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"Agence National de la Recherche","award":["AFRIPOX"],"award-info":[{"award-number":["AFRIPOX"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), in which outbreaks mainly occurred in West and Central Africa, with only sporadic spillovers to countries outside Africa due to international travel or close contact with wildlife. During May 2022, multiple countries in Europe, North and South America, Australia, Asia, and Africa reported near-simultaneous outbreaks of MPXV, the first time that patient clusters were reported over such a large geographical area. Cases have no known epidemiological links to MPXV-endemic countries in West or Central Africa. Real-time PCR is currently the gold standard for MPXV diagnostics, but it requires trained laboratory personnel and specialized equipment, and results can only be obtained after several hours. A rapid and simple-to-operate point-of-care diagnostic test for MPXV is crucial for limiting its spread and controlling outbreaks. Here, three recombinase-based isothermal amplification assays (RPA\/RAA) for the rapid detection of MPXV isolates were developed. These three assays target the MPXV G2R gene, and the limit of detection for these systems is approximately 100 copies of DNA per reaction. The assays were found to be specific and non-cross reactive against other pox viruses, such as vaccinia virus, and the results can be visualized within 20\u201330 min. The assays were validated with DNA extracted from 19 clinical samples from suspected or confirmed MPXV patients from Central Africa, and found to be consistent with findings from traditional qPCR. These results provide a solid platform for the early diagnosis of potential MPXV cases, and will help with the control and prevention of current and future outbreaks.<\/jats:p>","DOI":"10.3390\/v14102112","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T04:09:36Z","timestamp":1664424576000},"page":"2112","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA\/RAA) for the Rapid Detection of Monkeypox Virus"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2451-0569","authenticated-orcid":false,"given":"Lingjing","family":"Mao","sequence":"first","affiliation":[{"name":"Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens, Shanghai 200031, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxu","family":"Ying","sequence":"additional","affiliation":[{"name":"Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens, Shanghai 200031, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Selekon","sequence":"additional","affiliation":[{"name":"Institut Pasteur of Bangui, Bangui, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Gonofio","sequence":"additional","affiliation":[{"name":"Institut Pasteur of Bangui, Bangui, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Lanzhou University, Lanzhou 730000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Nakoune","sequence":"additional","affiliation":[{"name":"Institut Pasteur of Bangui, Bangui, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Wong","sequence":"additional","affiliation":[{"name":"Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Berthet","sequence":"additional","affiliation":[{"name":"Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens, Shanghai 200031, China"},{"name":"Institut Pasteur, Unit\u00e9 Environnement et Risque Infectieux, Cellule d\u2019Intervention Biologique d\u2019Urgence, 75724 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.2217\/17460913.2.1.17","article-title":"Human monkeypox: An emerging zoonotic disease","volume":"2","author":"Parker","year":"2007","journal-title":"Future Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1099\/vir.0.81215-0","article-title":"A tale of two clades: Monkeypox viruses","volume":"86","author":"Likos","year":"2005","journal-title":"J. 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Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/10\/2112\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:38:31Z","timestamp":1760143111000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/10\/2112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,23]]},"references-count":50,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["v14102112"],"URL":"https:\/\/doi.org\/10.3390\/v14102112","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9,23]]}}}