{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:19:01Z","timestamp":1790518741292,"version":"4.1.0"},"reference-count":23,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,28]],"date-time":"2022-09-28T00:00:00Z","timestamp":1664323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>(1) Background: What is the role of serum CMV PCR in the diagnosis of recent primary infection (PI) in pregnant women when IgG avidity is uninformative? (2) Methods: Retrospective cohort study to compare serum versus whole blood CMV PCR. (a) Qualitative assessment: CMV PCR was performed on 123 serum samples and 74 whole blood samples collected from 132 pregnant women with recent CMV PI. PCR positivity rate was used to calculate sensitivity in serum and whole blood. (b) Quantitative assessment: CMV PCR was performed on 72 paired samples of serum and whole blood collected on the same day from 57 patients. (3) Results: In pregnant women, PCR positivity rate was 89% for serum samples versus 100% in whole blood in the case of very recent PI (&lt;15 days), but only 27% in serum versus 68% in whole blood for PI occurring from 6 weeks to 3 months before. Comparing CMV viral loads between serum and whole blood, we determined the limit of CMV DNA detection in serum as 3 log copies\/mL (whole blood equivalent). (4) Conclusions: Serum CMV PCR is reliable in confirming PI in cases when only IgM is detected. It is therefore a valuable tool in introducing valaciclovir treatment as early as possible to prevent mother-to-child CMV transmission.<\/jats:p>","DOI":"10.3390\/v14102137","type":"journal-article","created":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T04:09:36Z","timestamp":1664424576000},"page":"2137","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Contribution of Serum Cytomegalovirus PCR to Diagnosis of Early CMV Primary Infection in Pregnant Women"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4711-5852","authenticated-orcid":false,"given":"Claire","family":"P\u00e9rillaud-Dubois","sequence":"first","affiliation":[{"name":"Laboratoire de Virologie, H\u00f4pital Universitaire Saint-Antoine, Institut Pierre Louis d\u2019Epid\u00e9miologie et de Sant\u00e9 Publique, Sorbonne Universit\u00e9, INSERM, AP-HP, 75018 Paris, France"},{"name":"Virology Laboratory, AP-HP, Sorbonne Universit\u00e9, H\u00f4pital Saint-Antoine, 75012 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Bouthry","sequence":"additional","affiliation":[{"name":"Virology Laboratory, CHU Angers, 49000 Angers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0749-769X","authenticated-orcid":false,"given":"Lina","family":"Mouna","sequence":"additional","affiliation":[{"name":"Virology Laboratory, AP-HP, Universit\u00e9 Paris-Saclay, H\u00f4pital Paul-Brousse, 94804 Villejuif, France"},{"name":"INSERM U-1193, Universit\u00e9 Paris-Saclay, 94804 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Pirin","sequence":"additional","affiliation":[{"name":"Virology Laboratory, AP-HP, Universit\u00e9 Paris-Saclay, H\u00f4pital Paul-Brousse, 94804 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Vieux-Combe","sequence":"additional","affiliation":[{"name":"Virology Laboratory, AP-HP, Universit\u00e9 Paris-Saclay, H\u00f4pital Paul-Brousse, 94804 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Picone","sequence":"additional","affiliation":[{"name":"Laboratoire de Virologie, H\u00f4pital Universitaire Saint-Antoine, Institut Pierre Louis d\u2019Epid\u00e9miologie et de Sant\u00e9 Publique, Sorbonne Universit\u00e9, INSERM, AP-HP, 75018 Paris, France"},{"name":"Division of Obstetrics and Gynecology, AP-HP, Nord, H\u00f4pital Louis Mourier, 92700 Colombes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6612-2030","authenticated-orcid":false,"given":"Anne-Marie","family":"Roque-Afonso","sequence":"additional","affiliation":[{"name":"Virology Laboratory, AP-HP, Universit\u00e9 Paris-Saclay, H\u00f4pital Paul-Brousse, 94804 Villejuif, France"},{"name":"INSERM U-1193, Universit\u00e9 Paris-Saclay, 94804 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4921-0047","authenticated-orcid":false,"given":"Alexandre J.","family":"Vivanti","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Gynecology, AP-HP, Universit\u00e9 Paris-Saclay, 92140 Clamart, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Vauloup-Fellous","sequence":"additional","affiliation":[{"name":"Virology Laboratory, AP-HP, Universit\u00e9 Paris-Saclay, H\u00f4pital Paul-Brousse, 94804 Villejuif, France"},{"name":"INSERM U-1193, Universit\u00e9 Paris-Saclay, 94804 Villejuif, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1002\/rmv.535","article-title":"Review and Meta-Analysis of the Epidemiology of Congenital Cytomegalovirus (CMV) Infection","volume":"17","author":"Kenneson","year":"2007","journal-title":"Rev. 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