{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:16:14Z","timestamp":1760231774484,"version":"build-2065373602"},"reference-count":27,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,10,5]],"date-time":"2022-10-05T00:00:00Z","timestamp":1664928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Research, Stavanger University Hospital","award":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"],"award-info":[{"award-number":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"]}]},{"DOI":"10.13039\/100016190","name":"Trond Mohn Stiftelse","doi-asserted-by":"publisher","award":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"],"award-info":[{"award-number":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"]}],"id":[{"id":"10.13039\/100016190","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Health and Care Services, Norway","award":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"],"award-info":[{"award-number":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"]}]},{"name":"Helse Vest, Norway","award":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"],"award-info":[{"award-number":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"]}]},{"name":"European Union","award":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"],"award-info":[{"award-number":["F12485-D10192","TMS2020TMT05","F-11628","F-12167","F-12621","H2020 101037867 Vaccelerate"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The aim of this study was to evaluate the performances of three commercially available antibody assays for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies at different time points following SARS-CoV-2 infection. Sera from 536 cases, including 207 SARS-CoV-2 PCR positive, were tested for SARS-CoV-2 antibodies with the Wantai receptor binding domain (RBD) total antibody assay, Liaison S1\/S2 IgG assay and Alinity i nucleocapsid IgG assay and compared to a two-step reference ELISA (SARS-CoV-2 RBD IgG and SARS-CoV-2 spike IgG). Diagnostic sensitivity, specificity, predictive values and Cohen\u2019s kappa were calculated for the commercial assays. The assay\u2019s sensitivities varied greatly, from 68.7% to 95.3%, but the specificities remained high (96.9\u201399.1%). The three tests showed good performances in sera sampled 31 to 60 days after PCR positivity compared to the reference ELISA. The total antibody test performed better than the IgG tests the first 30 days and the nucleocapsid IgG test showed reduced sensitivity two months or more after PCR positivity. Hence, the test performances at different time points should be taken into consideration in clinical practice and epidemiological studies. Spike or RBD IgG tests are preferable in sera sampled more than two months following SARS-CoV-2 infection.<\/jats:p>","DOI":"10.3390\/v14102196","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T02:10:27Z","timestamp":1665540627000},"page":"2196","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Performances of Three Commercially Available Assays for the Detection of SARS-CoV-2 Antibodies at Different Time Points Following SARS-CoV-2 Infection"],"prefix":"10.3390","volume":"14","author":[{"given":"Heidi","family":"Syre","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marius Eduardo Br\u00e5","family":"Obreque","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingvild","family":"Dalen","sequence":"additional","affiliation":[{"name":"Department of Research, Section of Biostatistics, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c5se Garl\u00f8v","family":"Riis","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6937-3580","authenticated-orcid":false,"given":"\u00c5se","family":"Berg","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iren H\u00f8yland","family":"L\u00f6hr","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon","family":"Sundal","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars K\u00e5re","family":"Kleppe","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"May Sissel","family":"Vadla","sequence":"additional","affiliation":[{"name":"Municipality of Randaberg, 4070 Randaberg, Norway"},{"name":"Faculty of Health Science, University of Stavanger, 4036 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ole Bernt","family":"Lenning","sequence":"additional","affiliation":[{"name":"Municipality of Randaberg, 4070 Randaberg, Norway"},{"name":"Department of Research, Stavanger University Hospital, 4068 Stavanger, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9772-4923","authenticated-orcid":false,"given":"Jan Stefan","family":"Olofsson","sequence":"additional","affiliation":[{"name":"Influenza Centre, Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristin Greve-Isdahl","family":"Mohn","sequence":"additional","affiliation":[{"name":"Influenza Centre, Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3389-3194","authenticated-orcid":false,"given":"Camilla","family":"T\u00f8ndel","sequence":"additional","affiliation":[{"name":"Influenza Centre, Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-4297","authenticated-orcid":false,"given":"Bj\u00f8rn","family":"Blomberg","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"},{"name":"Department of Medicine, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mai Chi","family":"Trieu","sequence":"additional","affiliation":[{"name":"Influenza Centre, Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Langeland","sequence":"additional","affiliation":[{"name":"Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"},{"name":"Department of Medicine, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8341-4078","authenticated-orcid":false,"given":"Rebecca Jane","family":"Cox","sequence":"additional","affiliation":[{"name":"Influenza Centre, Department of Clinical Science, University of Bergen, 5007 Bergen, Norway"},{"name":"Department of Microbiology, Haukeland University Hospital, 5021 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,5]]},"reference":[{"key":"ref_1","first-page":"Cd013652","article-title":"Cochraine COVID-19 Diagnostic Test Accuracy Group. 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Immunol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/10\/2196\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:47:00Z","timestamp":1760143620000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/10\/2196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,5]]},"references-count":27,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2022,10]]}},"alternative-id":["v14102196"],"URL":"https:\/\/doi.org\/10.3390\/v14102196","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2022,10,5]]}}}