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According to past research, A20 can both promote and inhibit tumor growth. In this study, DF-1 cells were infected with ALV-A rHB2015012, and Gp85 expression was measured at various time points. A recombinant plasmid encoding the chicken A20 gene and short hairpin RNA targeting chicken A20 (A20-shRNA) was constructed and transfected into DF-1 cells to determine the effect on ALV-A replication. The potential signaling pathways of A20 were explored using bioinformatics prediction, co-immunoprecipitation, and other techniques. The results demonstrate that A20 and ALV-A promoted each other after ALV-A infection of DF-1 cells, upregulated A20, inhibited TRAF6 ubiquitination, and promoted STAT3 phosphorylation. The phosphorylated-STAT3 (p-STAT3) promoted the expression of proto-oncogene c-myc, which may lead to tumorigenesis. This study will help to further understand the tumorigenic process of ALV-A and provide a reference for preventing and controlling ALV.<\/jats:p>","DOI":"10.3390\/v14102210","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T02:10:27Z","timestamp":1665540627000},"page":"2210","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A20 Enhances the Expression of the Proto-Oncogene C-Myc by Downregulating TRAF6 Ubiquitination after ALV-A Infection"],"prefix":"10.3390","volume":"14","author":[{"given":"Xueyang","family":"Chen","sequence":"first","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"},{"name":"College of Agriculture, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingming","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Fang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiongyan","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Yangtze University, No.88, Jingmi Road, Jingzhou 434025, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.3382\/ps\/pew180","article-title":"Viral proliferation and expression of tumor-related gene in different chicken embryo fibroblasts infected with different tumorigenic phenotypes of avian leukosis virus subgroup","volume":"95","author":"Qu","year":"2016","journal-title":"J. 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