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We investigated six lytic E. coli bacteriophages isolated from wastewater. Transmission electron microscopy and whole genome sequencing showed that the isolated bacteriophages are tailed phages of the Caudoviricetes class. One-step growth curves revealed that their latent period of reproduction is 20\u201330 min, and the average value of the burst size is 117\u2013155. During co-cultivation with various E. coli strains, the phages completely suppressed bacterial host culture growth within the first 4 h at MOIs 10\u22127 to 10\u22123. The host range lysed by each bacteriophage varied from six to two bacterial strains out of nine used in the study. The cocktail formed from the isolated bacteriophages possessed the ability to completely suppress the growth of all the E. coli strains used in the study within 6 h and maintain its lytic activity for 8 months of storage. All the isolated bacteriophages may be useful in fighting pathogenic E. coli strains and in the development of phage cocktails with a long storage period and high efficiency in the treatment of bacterial infections.<\/jats:p>","DOI":"10.3390\/v14112381","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T10:47:57Z","timestamp":1667126877000},"page":"2381","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Isolation and Characterization of Lytic Bacteriophages Active against Clinical Strains of E. coli and Development of a Phage Antimicrobial Cocktail"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3638-3341","authenticated-orcid":false,"given":"Pavel","family":"Alexyuk","sequence":"first","affiliation":[{"name":"Laboratory of Antiviral Protection, Department of Virology, Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Bogoyavlenskiy","sequence":"additional","affiliation":[{"name":"Laboratory of Antiviral Protection, Department of Virology, Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madina","family":"Alexyuk","sequence":"additional","affiliation":[{"name":"Laboratory of Antiviral Protection, Department of Virology, Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuralay","family":"Akanova","sequence":"additional","affiliation":[{"name":"Laboratory of Antiviral Protection, Department of Virology, Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yergali","family":"Moldakhanov","sequence":"additional","affiliation":[{"name":"Laboratory of Antiviral Protection, Department of Virology, Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Berezin","sequence":"additional","affiliation":[{"name":"Laboratory of Antiviral Protection, Department of Virology, Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Divatia, J.V., Pulinilkunnathil, J.G., and Myatra, S.N. 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