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Service","award":["21FED2100395IPD"],"award-info":[{"award-number":["21FED2100395IPD"]}]},{"name":"SCINet project of the USDA Agricultural Research Service","award":["DE-AC05- 06OR23100"],"award-info":[{"award-number":["DE-AC05- 06OR23100"]}]},{"name":"SCINet project of the USDA Agricultural Research Service","award":["0500-00093-001-00-D"],"award-info":[{"award-number":["0500-00093-001-00-D"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>During the last decade, endemic swine H1 influenza A viruses (IAV) from six different genetic clades of the hemagglutinin gene caused zoonotic infections in humans. The majority of zoonotic events with swine IAV were restricted to a single case with no subsequent transmission. However, repeated introduction of human-seasonal H1N1, continual reassortment between endemic swine IAV, and subsequent drift in the swine host resulted in highly diverse swine IAV with human-origin genes that may become a risk to the human population. To prepare for the potential of a future swine-origin IAV pandemic in humans, public health laboratories selected candidate vaccine viruses (CVV) for use as vaccine seed strains. To assess the pandemic risk of contemporary US swine H1N1 or H1N2 strains, we quantified the genetic diversity of swine H1 HA genes, and identified representative strains from each circulating clade. We then characterized the representative swine IAV against human seasonal vaccine and CVV strains using ferret antisera in hemagglutination inhibition assays (HI). HI assays revealed that 1A.3.3.2 (pdm09) and 1B.2.1 (delta-2) demonstrated strong cross reactivity to human seasonal vaccines or CVVs. However, swine IAV from three clades that represent more than 50% of the detected swine IAVs in the USA showed significant reduction in cross-reactivity compared to the closest CVV virus: 1A.1.1.3 (alpha-deletion), 1A.3.3.3-clade 3 (gamma), and 1B.2.2.1 (delta-1a). Representative viruses from these three clades were further characterized in a pig-to-ferret transmission model and shown to exhibit variable transmission efficiency. Our data prioritize specific genotypes of swine H1N1 and H1N2 to further investigate in the risk they pose to the human population.<\/jats:p>","DOI":"10.3390\/v14112398","type":"journal-article","created":{"date-parts":[[2022,10,30]],"date-time":"2022-10-30T10:47:57Z","timestamp":1667126877000},"page":"2398","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Interspecies Transmission from Pigs to Ferrets of Antigenically Distinct Swine H1 Influenza A Viruses with Reduced Reactivity to Candidate Vaccine Virus Antisera as Measures of Relative Zoonotic Risk"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1245-1530","authenticated-orcid":false,"given":"J. Brian","family":"Kimble","sequence":"first","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carine K.","family":"Souza","sequence":"additional","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3138-5535","authenticated-orcid":false,"given":"Tavis K.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zebulun W.","family":"Arendsee","sequence":"additional","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6221-2493","authenticated-orcid":false,"given":"David E.","family":"Hufnagel","sequence":"additional","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katharine M.","family":"Young","sequence":"additional","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola S.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Pathology and Population Sciences, Royal Veterinary College, University of London, London NW1 0TU, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Todd","family":"Davis","sequence":"additional","affiliation":[{"name":"Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharmi","family":"Thor","sequence":"additional","affiliation":[{"name":"Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4953-7285","authenticated-orcid":false,"given":"Amy L.","family":"Vincent Baker","sequence":"additional","affiliation":[{"name":"Virus Prion Research Unit, National Animal Disease Center, United States Department of Agriculture-ARS, Ames, IA 50010, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.1056\/NEJMra1002842","article-title":"Influenza vaccines for the future","volume":"363","author":"Lambert","year":"2010","journal-title":"N. 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