{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:15:14Z","timestamp":1787030114520,"version":"build-2736575974"},"reference-count":26,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federal Service for the Protection of Consumer Rights and Human Welfare"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The aim of the work was an experimental evaluation of the characteristics of the kit for the rapid immunochemical detection of orthopoxviruses (OPV). The kit is based on the method of one-stage dot-immunoassay on flat protein arrays using gold conjugates and a silver developer. Rabbit polyclonal antibodies against the vaccinia virus were used as capture and detection reagents. The sensitivity of detection of OPV and the specificity of the analysis were assessed using culture crude preparations (monkeypox virus, vaccinia virus, rabbitpox virus, cowpox virus, and ectromelia virus), a suspension from a crust from a human vaccination site as well as blood and tissue suspensions of infected rabbits. It has been shown that the assay using the kit makes it possible to detect OPV within 36 min at a temperature of 18\u201340 \u00b0C in unpurified culture samples of the virus and clinical samples in the range of 103\u2013104 PFU\/mL. Tests of the kit did not reveal cross-reactivity with uninfected cell cultures and viral pathogens of exanthematous infections (measles, rubella and chicken pox). The kit can be used to detect or exclude the presence of a virus threat in samples and can be useful in various aspects of biosecurity. The simplicity of analysis, the possibility of visual accounting the and interpretation of the results make it possible to use the test in laboratories with a high level of biological protection and in out-of-laboratory conditions.<\/jats:p>","DOI":"10.3390\/v14112580","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T03:04:20Z","timestamp":1669086260000},"page":"2580","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of Rapid Dot-Immunoassay for Detection Orthopoxviruses Using Laboratory-Grown Viruses and Animal\u2019s Clinical Specimens"],"prefix":"10.3390","volume":"14","author":[{"given":"Nikita","family":"Ushkalenko","sequence":"first","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Novosibirsk Region, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Ersh","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Novosibirsk Region, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8355-5551","authenticated-orcid":false,"given":"Alexander","family":"Sergeev","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Novosibirsk Region, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7763-3808","authenticated-orcid":false,"given":"Pavel","family":"Filatov","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Novosibirsk Region, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Poltavchenko","sequence":"additional","affiliation":[{"name":"State Research Center of Virology and Biotechnology \u201cVector\u201d, Novosibirsk Region, Koltsovo 630559, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0166-3542(02)00195-X","article-title":"Smallpox: A Potential Agent of Bioterrorism","volume":"57","author":"Whitley","year":"2003","journal-title":"Antivir. 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