{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T02:39:09Z","timestamp":1791686349628,"version":"4.3.4"},"reference-count":85,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["21-14-00245"],"award-info":[{"award-number":["21-14-00245"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In this work, we presented data from a two-year study of flavi-, flavi-like, and phenuiviruses circulation in the population of ixodid ticks in the Chelyabinsk region. We isolated three tick-borne encephalitis virus (TBEV) strains from I. persulcatus, which was not detected in the ticks of the genus Dermacentor. The virus prevalence ranged from 0.66% to 2.28%. The Yanggou tick virus (YGTV) is widespread in steppe and forest-steppe zones and is mainly associated with ticks of the genus Dermacentor. We isolated 26 strains from D. reticulatus, D. marginatus, and I. persulcatus ticks in the HAE\/CTVM8 tick cell line. The virus prevalence ranged from 1.58% to 4.18% in D. reticulatus, ranged from 0.78% to 3.93% in D. marginatus, and was 0.66% in I. persulcatus. There was combined focus of TBEV and YGTV in the territory of the Chelyabinsk region. The Alongshan virus (ALSV) was found to be associated with I. persulcatus ticks and is spread in forest zone. We detected 12 amplicons and isolated 7 strains of ALSV in tick cells. The virus prevalence ranged from 1.13% to 6.00%. The phlebovirus Gomselga and unclassified phenuivirus Stavropol were associated with I. persulcatus and D. reticulatus ticks, respectively. Virus prevalence of the unclassified phenuivirus Stavropol in the Chelyabinsk region is lower than that in neighbouring regions.<\/jats:p>","DOI":"10.3390\/v14122699","type":"journal-article","created":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T05:30:46Z","timestamp":1669872646000},"page":"2699","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Distribution and Characterisation of Tick-Borne Flavi-, Flavi-like, and Phenuiviruses in the Chelyabinsk Region of Russia"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3764-7081","authenticated-orcid":false,"given":"Ivan","family":"Kholodilov","sequence":"first","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9040-0774","authenticated-orcid":false,"given":"Oxana","family":"Belova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Ivannikova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1571-2313","authenticated-orcid":false,"given":"Magomed","family":"Gadzhikurbanov","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"},{"name":"Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6835-4572","authenticated-orcid":false,"given":"Marat","family":"Makenov","sequence":"additional","affiliation":[{"name":"Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Yakovlev","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Polienko","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9612-2146","authenticated-orcid":false,"given":"Alena","family":"Dereventsova","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Litov","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Gmyl","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egor","family":"Okhezin","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"},{"name":"Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Luchinina","sequence":"additional","affiliation":[{"name":"Office of Rospotrebnadzor in the Chelyabinsk Region, 454092 Chelyabinsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Klimentov","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemistry, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Karganova","sequence":"additional","affiliation":[{"name":"Laboratory of Biology of Arboviruses, FSASI Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1128\/JVI.02036-15","article-title":"Divergent Viruses Discovered in Arthropods and Vertebrates Revise the Evolutionary History of the Flaviviridae and Related Viruses","volume":"90","author":"Shi","year":"2016","journal-title":"J. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1016\/S1473-3099(14)70718-2","article-title":"Severe fever with thrombocytopenia syndrome, an emerging tick-borne zoonosis","volume":"14","author":"Liu","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1186\/s12985-020-01327-9","article-title":"First report of Lihan Tick virus (Phlebovirus, Phenuiviridae) in ticks, Colombia","volume":"17","author":"Miranda","year":"2020","journal-title":"Virol. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"6744","DOI":"10.1073\/pnas.1324194111","article-title":"A tick-borne segmented RNA virus contains genome segments derived from unsegmented viral ancestors","volume":"111","author":"Qin","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Litov, A.G., Belova, O.A., Kholodilov, I.S., Gadzhikurbanov, M.N., Gmyl, L.V., Oorzhak, N.D., Saryglar, A.A., Ishmukhametov, A.A., and Karganova, G.G. (2021). Possible Arbovirus Found in Virome of Melophagus ovinus. Viruses, 13.","DOI":"10.3390\/v13122375"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.ebiom.2019.04.004","article-title":"Emergence of human infection with Jingmen tick virus in China: A retrospective study","volume":"43","author":"Jia","year":"2019","journal-title":"EBioMedicine"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1056\/NEJMoa1805068","article-title":"A New Segmented Virus Associated with Human Febrile Illness in China","volume":"380","author":"Wang","year":"2019","journal-title":"N. Engl. J. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1056\/NEJMoa1203378","article-title":"A New Phlebovirus Associated with Severe Febrile Illness in Missouri","volume":"367","author":"McMullan","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1099\/jgv.0.000672","article-title":"ICTV Virus Taxonomy Profile: Flaviviridae","volume":"98","author":"Simmonds","year":"2017","journal-title":"J. Gen. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2104","DOI":"10.1016\/S0140-6736(10)61120-8","article-title":"Omsk haemorrhagic fever","volume":"376","author":"Yakimenko","year":"2010","journal-title":"Lancet"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.antiviral.2019.01.014","article-title":"Tick-borne encephalitis in Europe and Russia: Review of pathogenesis, clinical features, therapy, and vaccines","volume":"164","author":"Ruzek","year":"2019","journal-title":"Antiviral. Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"376230","DOI":"10.1155\/2015\/376230","article-title":"The global ecology and epidemiology of West Nile virus","volume":"2015","author":"Chancey","year":"2015","journal-title":"Biomed. Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.2741\/4882","article-title":"Japanese encephalitis virus: A review on emerging diagnostic techniques","volume":"25","author":"Roberts","year":"2020","journal-title":"Front. Biosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/S0140-6736(14)60572-9","article-title":"Dengue","volume":"385","author":"Guzman","year":"2015","journal-title":"Lancet"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1111\/j.1749-6632.2001.tb02689.x","article-title":"West Nile encephalitis in Russia 1999\u20132001: Were we ready? Are we ready?","volume":"951","author":"Platonov","year":"2001","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Shartova, N., Mironova, V., Zelikhina, S., Korennoy, F., and Grishchenko, M. (2022). Spatial patterns of West Nile virus distribution in the Volgograd region of Russia, a territory with long-existing foci. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010145"},{"key":"ref_17","first-page":"52","article-title":"Japanese encephalitis, as a problem of reemerging arboviral neuroinfections on border territory of Far East Russian and China","volume":"68","author":"Demenev","year":"2016","journal-title":"Zdr. Dal\u2019Nego Vostoka"},{"key":"ref_18","first-page":"5","article-title":"The Omsk hemorrhagic fever: Research results (1946\u20132013)","volume":"59","author":"Yastrebov","year":"2014","journal-title":"Vopr. Virusol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1007\/s00705-009-0376-y","article-title":"Characterization of Powassan viruses from Far Eastern Russia","volume":"154","author":"Leonova","year":"2009","journal-title":"Arch. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s00705-014-2310-1","article-title":"Louping ill virus (LIV) in the Far East","volume":"160","author":"Leonova","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Din\u00e7er, E., Hac\u0131o\u011flu, S., Kar, S., Emanet, N., Brinkmann, A., Nitsche, A., \u00d6zkul, A., Linton, Y.-M., and Erg\u00fcnay, K. (2019). Survey and Characterization of Jingmen Tick Virus Variants. Viruses, 11.","DOI":"10.3390\/v11111071"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Garry, C.E., and Garry, R.F. (2020). Proteomics Computational Analyses Suggest that the Envelope Glycoproteins of Segmented Jingmen Flavi-Like Viruses Are Class II Viral Fusion Proteins (\u03b2-Penetrenes) with Mucin-Like Domains. Viruses, 12.","DOI":"10.20944\/preprints202002.0114.v1"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Kholodilov, I.S., Belova, O.A., Morozkin, E.S., Litov, A.G., Ivannikova, A.Y., Makenov, M.T., Shchetinin, A.M., Aibulatov, S.V., Bazarova, G.K., and Bell-Sakyi, L. (2021). Geographical and Tick-Dependent Distribution of Flavi-like Alongshan and Yanggou Tick Viruses in Russia. Viruses, 13.","DOI":"10.3390\/v13030458"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/mSphere.00645-19","article-title":"Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses","volume":"4","author":"Temmam","year":"2019","journal-title":"mSphere"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1900394","DOI":"10.2807\/1560-7917.ES.2019.24.27.1900394","article-title":"Detection of novel tick-borne pathogen, Alongshan virus, in Ixodes ricinus ticks, south-eastern Finland","volume":"24","author":"Kuivanen","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1128\/genomeA.00232-17","article-title":"Complete Coding Genome Sequence for Mogiana Tick Virus, a Jingmenvirus Isolated from Ticks in Brazil","volume":"5","author":"Villa","year":"2017","journal-title":"Genome Announc."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/j.chom.2016.07.011","article-title":"A Multicomponent Animal Virus Isolated from Mosquitoes","volume":"20","author":"Ladner","year":"2016","journal-title":"Cell Host. Microbe."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Ogola, E.O., Kopp, A., Bastos, A.D.S., Slothouwer, I., Marklewitz, M., Omoga, D., Rotich, G., Getugi, C., Sang, R., and Torto, B. (2022). Jingmen Tick Virus in Ticks from Kenya. Viruses, 14.","DOI":"10.3390\/v14051041"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"16315","DOI":"10.1038\/s41598-018-34630-1","article-title":"Viral diversity of Rhipicephalus microplus parasitizing cattle in southern Brazil","volume":"8","author":"Fumagalli","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1186\/s13071-019-3707-1","article-title":"Prevalence of the emerging novel Alongshan virus infection in sheep and cattle in Inner Mongolia, northeastern China","volume":"12","author":"Wang","year":"2019","journal-title":"Parasit. Vectors"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1590\/0074-0276130166","article-title":"Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil","volume":"109","author":"Maruyama","year":"2014","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/j.meegid.2018.07.010","article-title":"Viral metagenomics, genetic and evolutionary characteristics of Crimean-Congo hemorrhagic fever orthonairovirus in humans, Kosovo","volume":"65","author":"Emmerich","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kholodilov, I.S., Litov, A.G., Klimentov, A.S., Belova, O.A., Polienko, A.E., Nikitin, N.A., Shchetinin, A.M., Ivannikova, A.Y., Bell-Sakyi, L., and Yakovlev, A.S. (2020). Isolation and characterisation of Alongshan virus in Russia. Viruses, 12.","DOI":"10.3390\/v12040362"},{"key":"ref_34","first-page":"129","article-title":"Detection of the RNA for New Multicomponent Virus in Patients with Crimean-Congo Hemorrhagic Fever in Southern Russia","volume":"75","author":"Ternovoi","year":"2020","journal-title":"Vestn. Ross. Akad. Meditsinskikh Nauk"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3023","DOI":"10.1007\/s00705-020-04731-2","article-title":"2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales","volume":"165","author":"Kuhn","year":"2020","journal-title":"Arch. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1099\/jgv.0.001296","article-title":"Rift Valley fever: Biology and epidemiology","volume":"100","author":"Wright","year":"2019","journal-title":"J. Gen. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"104524","DOI":"10.1016\/j.meegid.2020.104524","article-title":"Phlebovirus sequences detected in ticks collected in the Russian Federation: Novel species, distinguish criteria, high tick specificity","volume":"85","author":"Klimentov","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"102045","DOI":"10.1016\/j.ttbdis.2022.102045","article-title":"Ticks and Tick-borne Diseases Pathogens in Ixodes persulcatus and Ixodes ricinus ticks (Acari, Ixodidae) in Karelia","volume":"13","author":"Bugmyrin","year":"2022","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"101607","DOI":"10.1016\/j.ttbdis.2020.101607","article-title":"Tick-borne diseases and co-infection: Current considerations","volume":"12","author":"Cutler","year":"2021","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1186\/s13071-020-04219-7","article-title":"Large-scale countrywide screening for tick-borne pathogens in field-collected ticks in Latvia during 2017\u20132019","volume":"13","author":"Capligina","year":"2020","journal-title":"Parasites Vectors"},{"key":"ref_41","unstructured":"(2022, June 02). General Characteristics of the Chelyabinsk Region. Available online: https:\/\/chelreglib.ru\/ru\/pages\/kray\/chelregion\/general\/."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s10493-020-00533-y","article-title":"Vectors of disease at the northern distribution limit of the genus Dermacentor in Eurasia: D. reticulatus and D. silvarum","volume":"82","author":"Rubel","year":"2020","journal-title":"Exp. Appl. Acarol."},{"key":"ref_43","unstructured":"Luchinina, S.V., Feldblyum, I.V., and Usoltseva, N.M. (2015). The role of ticks of the genus Dermacentor in transmission of tick-borne encephalitis in the Southern Urals. Results and Prospects of Studying the Problems of Infectious and Parasitic Diseases, University of Tyumen. (In Russian)."},{"key":"ref_44","first-page":"32","article-title":"Modern Epidemiological Situation of Tick-Borne Encephalitis in Chelyabinsk Region, Russia","volume":"75","author":"Luchinina","year":"2014","journal-title":"Epidemiol. Vaccinal Prev."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1134\/S0013873820080126","article-title":"Types of Parasitism of Hard Ticks (Ixodidae)","volume":"100","author":"Leonovich","year":"2020","journal-title":"Entomol. Rev."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"67","DOI":"10.31631\/2073-3046-2016-15-1-67-76","article-title":"Vaccinal Prevention of Tick-Borne Encephalitis in Chelyabinsk Region: Dynamics of Vaccination, Population Immunity, Analysis of TBE Cases in Vaccinated Persons","volume":"86","author":"Luchinina","year":"2016","journal-title":"Epidemiol. Vaccinal Prev."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"41","DOI":"10.3103\/S0891416811010071","article-title":"Quantitations of Borrelia and Bartonella DNA and tick-borne encephalitis virus RNA in Ixodes persulcatus ticks collected in Chelyabinsk region","volume":"26","author":"Morozova","year":"2011","journal-title":"Mol. Genet. Microbiol. Virol."},{"key":"ref_48","unstructured":"Filippova, N.A. (1977). Ixodid Ticks of the Subfamily Ixodinae. Fauna of the USSR: Arachnoides, Nauka. (In Russian)."},{"key":"ref_49","unstructured":"Filippova, N.A. (1997). Fauna of Russia and Neighbouring Countries. Ixodid Ticks of Subfamily Amblyomminae. Arachnoidea, Nauka. (In Russian)."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.2307\/3282757","article-title":"Continuous cell lines from the tick Hyalomma anatolicum anatolicum","volume":"77","year":"1991","journal-title":"J. Parasitol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.ttbdis.2019.04.019","article-title":"Ixodid ticks and tick-borne encephalitis virus prevalence in the South Asian part of Russia (Republic of Tuva)","volume":"10","author":"Kholodilov","year":"2019","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1128\/JCM.39.5.1922-1927.2001","article-title":"Comparison of Flavivirus universal primer pairs and development of a rapid, highly sensitive heminested reverse transcription-PCR assay for detection of flaviviruses targeted to a conserved region of the NS5 gene sequences","volume":"39","author":"Scaramozzino","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.jviromet.2016.02.009","article-title":"Development of pan-phlebovirus RT-PCR assay","volume":"232","author":"Klimentov","year":"2016","journal-title":"J. Virol. Methods"},{"key":"ref_54","first-page":"38","article-title":"Evidence for the absence of tick-borne encephalitis virus RNA in bioassays","volume":"51","author":"Romanova","year":"2006","journal-title":"Vopr. Virusol."},{"key":"ref_55","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura","year":"1993","journal-title":"Mol. Biol. Evol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1577\/1548-8667(2001)013<0300:ACOMOS>2.0.CO;2","article-title":"A Critique of Methods of Sampling and Reporting Pathogens in Populations of Fish","volume":"13","author":"Williams","year":"2001","journal-title":"J. Aquat. Anim. Health"},{"key":"ref_58","unstructured":"R Core Team (2022, June 02). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. Available online: https:\/\/www.r-project.org\/."},{"key":"ref_59","unstructured":"QGIS Development Team (2022, May 24). QGIS Geographic Information System. Open Source Geospatial Foundation Project. Available online: http:\/\/qgis.osgeo.org."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ttbdis.2013.09.003","article-title":"Ixodes ricinus and Borrelia prevalence at the Arctic Circle in Norway","volume":"5","author":"Hvidsten","year":"2014","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1111\/apm.12412","article-title":"Prevalence of tick-borne encephalitis virus in Ixodes ricinus ticks from three islands in north-western Norway","volume":"123","author":"Paulsen","year":"2015","journal-title":"Apmis"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.ttbdis.2017.10.002","article-title":"Distribution of Ixodes ricinus ticks and prevalence of tick-borne encephalitis virus among questing ticks in the Arctic Circle region of northern Norway","volume":"9","author":"Soleng","year":"2018","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1900679","DOI":"10.2807\/1560-7917.ES.2019.24.47.1900679","article-title":"A probable case of tick-borne encephalitis (TBE) acquired in England, July 2019","volume":"24","author":"Kreusch","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3201\/eid2601.191085","article-title":"Tick-borne encephalitis virus, United Kingdom","volume":"26","author":"Holding","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1900658","DOI":"10.2807\/1560-7917.ES.2019.24.47.1900658","article-title":"Detection of new endemic focus of tick-borne encephalitis virus (TBEV), Hampshire\/Dorset border, England, September 2019","volume":"24","author":"Holding","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.3201\/eid2306.161247","article-title":"Tick-borne encephalitis virus in ticks and roe deer, the Netherlands","volume":"23","author":"Jahfari","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.ttbdis.2018.10.008","article-title":"Emergence of tick-borne encephalitis (TBE) in the Netherlands","volume":"10","author":"Dekker","year":"2019","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"101265","DOI":"10.1016\/j.ttbdis.2019.101265","article-title":"First detection of tick-borne encephalitis virus in Ixodes ricinus ticks and their rodent hosts in Moscow, Russia","volume":"10","author":"Makenov","year":"2019","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Khamassi Khbou, M., Romdhane, R., Foughali, A.A., Sassi, L., Suin, V., Rekik, M., and Benzarti, M. (2020). Presence of antibodies against tick-borne encephalitis virus in sheep in Tunisia, North Africa. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-02651-6"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"101606","DOI":"10.1016\/j.ttbdis.2020.101606","article-title":"Tick-borne encephalitis virus in Ixodes ricinus (Acari: Ixodidae) ticks, Tunisia","volume":"12","author":"Fares","year":"2021","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"56","DOI":"10.17816\/EID40869","article-title":"Unusual case of lethal tick-borne encephalitis in patient vaccinated with vaccines produced from different viruses strains (the Chelyabinsk Region)","volume":"20","author":"Pogodina","year":"2015","journal-title":"Epidemiohgiya Indectsionnye Bolezn."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.ttbdis.2015.02.005","article-title":"Influence of altitude on tick-borne encephalitis infection risk in the natural foci of the Altai Republic, Southern Siberia","volume":"6","author":"Shchuchinova","year":"2015","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ttbdis.2010.10.001","article-title":"Prevalence of tick-borne encephalitis virus in Ixodes ricinus and Dermacentor reticulatus ticks collected from the Lublin region (eastern Poland)","volume":"2","author":"Cisak","year":"2011","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1134\/S0013873818090142","article-title":"The Ability of Ixodid Ticks (Acari: Ixodidae) to Support Reproduction of the Tick-Borne Encephalitis Virus","volume":"98","author":"Belova","year":"2018","journal-title":"Entomol. Rev."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1016\/j.ttbdis.2017.07.008","article-title":"Properties of the tick-borne encephalitis virus population during persistent infection of ixodid ticks and tick cell lines","volume":"8","author":"Belova","year":"2017","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_76","unstructured":"Valitskaya, A.V., Katin, A.A., Bragina, E.A., and Shuman, V.A. (2016, January 10). Comparative study of the role of the ixodid ticks Ixodes persulcatus and Dermacentor reticulatus in the epidemiological process of tick-borne encephalitis in the western part of Western Siberia. Proceedings of the VIII International Scientifc Conference on \u2018Reading in Memory of Professor I. I. Barabash-Nikiforov\u2019, Voronezh, Russian. (In Russian)."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1016\/j.ttbdis.2015.10.015","article-title":"Geographical distribution of Dermacentor marginatus and Dermacentor reticulatus in Europe","volume":"7","author":"Rubel","year":"2016","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1089\/vbz.2021.0092","article-title":"Dermacentor silvarum, a Medically Important Tick, May Not Be a Competent Vector to Transmit Jingmen Tick Virus","volume":"22","author":"Li","year":"2022","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"veaa051","DOI":"10.1093\/ve\/veaa051","article-title":"Diversity and circulation of Jingmen tick virus in ticks and mammals","volume":"6","author":"Guo","year":"2020","journal-title":"Virus Evol."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Morozkin, E.S., Makenov, M.T., Zhurenkova, O.B., Kholodilov, I.S., Belova, O.A., Radyuk, E.V., Fyodorova, M.V., Grigoreva, Y.E., Litov, A.G., and Valdokhina, A.V. (2022). Integrated Jingmenvirus Polymerase Gene in Ixodes ricinus Genome. Viruses, 14.","DOI":"10.3390\/v14091908"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.ttbdis.2016.05.006","article-title":"Diapause in ticks of the medically important Ixodes ricinus species complex","volume":"7","author":"Gray","year":"2016","journal-title":"Ticks Tick. Borne. Dis."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1023\/A:1006070416135","article-title":"The ecology of ticks transmitting Lyme borreliosis","volume":"22","author":"Gray","year":"1998","journal-title":"Exp. Appl. Acarol."},{"key":"ref_83","first-page":"84","article-title":"Seasonal Life Cycle Organisation of the Ixodid Tick Dermacentor reticulatus in Central Europe Implications on Its Vector Role and Distribution","volume":"12","author":"Kahl","year":"2013","journal-title":"Med. Kuzbass"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1186\/s13071-016-1599-x","article-title":"Dermacentor reticulatus: A vector on the rise","volume":"9","author":"Szekeres","year":"2016","journal-title":"Parasites and Vectors"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1016\/j.ttbdis.2018.11.012","article-title":"Infection of newly identified phleboviruses in ticks and wild animals in Hokkaido, Japan indicating tick-borne life cycles","volume":"10","author":"Torii","year":"2019","journal-title":"Ticks Tick. Borne. Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/12\/2699\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:32:02Z","timestamp":1760146322000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/14\/12\/2699"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,1]]},"references-count":85,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["v14122699"],"URL":"https:\/\/doi.org\/10.3390\/v14122699","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,1]]}}}