{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T17:58:53Z","timestamp":1789408733475,"version":"build-2803163510"},"reference-count":36,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T00:00:00Z","timestamp":1670371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China Grant","award":["U1905204"],"award-info":[{"award-number":["U1905204"]}]},{"name":"National Natural Science Foundation of China Grant","award":["CARS-47"],"award-info":[{"award-number":["CARS-47"]}]},{"name":"National Natural Science Foundation of China Grant","award":["2021F02"],"award-info":[{"award-number":["2021F02"]}]},{"name":"National Natural Science Foundation of China Grant","award":["2021N5008"],"award-info":[{"award-number":["2021N5008"]}]},{"name":"China Agriculture Research System of MOF and MARA Grant","award":["U1905204"],"award-info":[{"award-number":["U1905204"]}]},{"name":"China Agriculture Research System of MOF and MARA Grant","award":["CARS-47"],"award-info":[{"award-number":["CARS-47"]}]},{"name":"China Agriculture Research System of MOF and MARA Grant","award":["2021F02"],"award-info":[{"award-number":["2021F02"]}]},{"name":"China Agriculture Research System of MOF and MARA Grant","award":["2021N5008"],"award-info":[{"award-number":["2021N5008"]}]},{"name":"Institute of Oceanology of Fuzhou","award":["U1905204"],"award-info":[{"award-number":["U1905204"]}]},{"name":"Institute of Oceanology of Fuzhou","award":["CARS-47"],"award-info":[{"award-number":["CARS-47"]}]},{"name":"Institute of Oceanology of Fuzhou","award":["2021F02"],"award-info":[{"award-number":["2021F02"]}]},{"name":"Institute of Oceanology of Fuzhou","award":["2021N5008"],"award-info":[{"award-number":["2021N5008"]}]},{"name":"Fujian Science and Technology Department Grants","award":["U1905204"],"award-info":[{"award-number":["U1905204"]}]},{"name":"Fujian Science and Technology Department Grants","award":["CARS-47"],"award-info":[{"award-number":["CARS-47"]}]},{"name":"Fujian Science and Technology Department Grants","award":["2021F02"],"award-info":[{"award-number":["2021F02"]}]},{"name":"Fujian Science and Technology Department Grants","award":["2021N5008"],"award-info":[{"award-number":["2021N5008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel (Anguilla rostrata), its pathogenicity has not been well characterized. In this study, an AngHV-1 isolate, tentatively named AngHV-1-FC, was isolated from diseased American eels with similar symptoms as those observed in AngHV-1-infected European eels and Japanese eels. AngHV-1-FC induced severe cytopathic effects in the European eel spleen cell line (EES), and numerous concentric circular virions were observed in the infected EES cells by transmission electron microscopy. Moreover, AngHV-1-FC caused the same symptoms as the naturally diseased European eels and Japanese eels through experimental infection, resulting in a 100% morbidity rate and 13.3% mortality rate. The whole genome sequence analyses showed that the average nucleotide identity value between AngHV-1-FC and other AngHV-1 isolates ranged from 99.28% to 99.55%. However, phylogenetic analysis revealed that there was a genetic divergence between AngHV-1-FC and other AngHV-1 isolates, suggesting that AngHV-1-FC was a new isolate of AngHV-1. Thus, our results indicated that AngHV-1-FC can infect farmed American eels, with a high pathogenicity, providing new knowledge in regard to the prevalence and prevention of AngHV-1.<\/jats:p>","DOI":"10.3390\/v14122722","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T02:49:35Z","timestamp":1670381375000},"page":"2722","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5186-7224","authenticated-orcid":false,"given":"Rui","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Fuzhou Ocean and Fisheries Technology Center, Fuzhou 350007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4437-5309","authenticated-orcid":false,"given":"Tianliang","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Provincial Fishery Technical Extension Center, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchen","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Freshwater Fisheries Research Institute of Fujian Province, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Fuzhou Ocean and Fisheries Technology Center, Fuzhou 350007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiping","family":"Fan","sequence":"additional","affiliation":[{"name":"Freshwater Fisheries Research Institute of Fujian Province, Fuzhou 350002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China"},{"name":"Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,7]]},"reference":[{"key":"ref_1","unstructured":"Zhong, Q., Fan, H., Lin, Y., and Cai, L. 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