{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T02:31:39Z","timestamp":1787452299835,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T00:00:00Z","timestamp":1671667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research &amp; Development Program of China","award":["2022YFD1800300"],"award-info":[{"award-number":["2022YFD1800300"]}]},{"name":"National Key Research &amp; Development Program of China","award":["212300410353"],"award-info":[{"award-number":["212300410353"]}]},{"name":"National Key Research &amp; Development Program of China","award":["23A230004"],"award-info":[{"award-number":["23A230004"]}]},{"name":"Natural Science Foundation of Henan Province","award":["2022YFD1800300"],"award-info":[{"award-number":["2022YFD1800300"]}]},{"name":"Natural Science Foundation of Henan Province","award":["212300410353"],"award-info":[{"award-number":["212300410353"]}]},{"name":"Natural Science Foundation of Henan Province","award":["23A230004"],"award-info":[{"award-number":["23A230004"]}]},{"name":"Key scientific research projects of colleges and universities in Henan Province","award":["2022YFD1800300"],"award-info":[{"award-number":["2022YFD1800300"]}]},{"name":"Key scientific research projects of colleges and universities in Henan Province","award":["212300410353"],"award-info":[{"award-number":["212300410353"]}]},{"name":"Key scientific research projects of colleges and universities in Henan Province","award":["23A230004"],"award-info":[{"award-number":["23A230004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The antibody-dependent enhancement (ADE) effect of a PRRSV infection is that the preexisting sub- or non-neutralizing antibodies specific against PRRSV can facilitate the virus entry and replication, and it is likely to be a great obstacle for the selection of immune strategies and the development of high-efficiency PRRSV vaccines. However, the proteomic characterization of primary alveolar macrophages (PAMs) with a PRRSV-ADE infection has not yet been investigated so far. Therefore, we performed a tandem mass tag (TMT)-based quantitative proteomic analysis of PAMs with a PRRSV-ADE infection in this study. The results showed that a total of 3935 differentially expressed proteins (DEPs) were identified in the PAMs infected with PRRSV-ADE, including 2004 up-regulated proteins and 1931 down-regulated proteins. Further, the bioinformatics analysis for these DEPs revealed that a PRRSV-ADE infection might disturb the functions of ribosome, proteasome and mitochondria. Interestingly, we also found that the expression of the key molecules in the innate immune pathways and antiviral proteins were significantly down-regulated during a PRRSV-ADE infection. This study was the first attempt to analyze the proteomic characterization of PAMs with a PRRSV-ADE infection in vitro. Additionally, the findings will provide valuable information for a better understanding of the mechanism of virus\u2013antibody\u2013host interactions during a PRRSV-ADE infection.<\/jats:p>","DOI":"10.3390\/v15010036","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T02:36:46Z","timestamp":1671763006000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Proteomic Characterization of PAMs with PRRSV-ADE Infection"],"prefix":"10.3390","volume":"15","author":[{"given":"Pengli","family":"Xu","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiying","family":"Cui","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2993-7270","authenticated-orcid":false,"given":"Yi-na","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Science, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingan","family":"Xia","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Longzi Lake 15#, Zhengzhou 450046, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.vetimm.2004.09.017","article-title":"Monoclonal antibody analysis of porcine reproductive and respiratory syndrome virus epitopes associated with antibody-dependent enhancement and neutralization of virus infection","volume":"102","author":"Evans","year":"2004","journal-title":"Vet. 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