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It has never been reared beyond an F1 generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F3 generation Hg. janthinomys eggs for the first time. A total of 62.8% (1562\/2486) F1 generation eggs hatched during \u226410 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190\/944) in the F2 generation for eggs laid by mosquitoes copulated by forced mating. More than 85% (79\/92) female F2 mosquitoes fed on an artificial blood feeding system. While we were unable to maintain a laboratory colony of Hg. janthinomys past the F3 generation, our methods provide a foundation for experimental transmission studies with this species in a laboratory setting, a critical capacity in a region with hyper-endemic transmission of dengue, Zika, and chikungunya viruses, all posing a risk of spillback into a sylvatic cycle.<\/jats:p>","DOI":"10.3390\/v15010045","type":"journal-article","created":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T03:55:21Z","timestamp":1671767721000},"page":"45","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Towards the Laboratory Maintenance of Haemagogus janthinomys (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8082-6352","authenticated-orcid":false,"given":"Adam","family":"Hendy","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9099-8403","authenticated-orcid":false,"given":"Nelson Ferreira","family":"F\u00e9","sequence":"additional","affiliation":[{"name":"Centro de Entomologia, Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Val\u00e9rio","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Hernandez-Acosta","sequence":"additional","affiliation":[{"name":"Department of Biology, New Mexico State University, Las Cruces, NM 88003-8801, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2079-6096","authenticated-orcid":false,"given":"B\u00e1rbara A.","family":"Chaves","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds Felipe Alho","family":"da Silva","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Am\u00e9lia Gon\u00e7alves","family":"Santana","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9ia","family":"da Costa Paz","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2355-1441","authenticated-orcid":false,"given":"Matheus Mickael Mota","family":"Soares","sequence":"additional","affiliation":[{"name":"Graduate Program in Entomology, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69067-375, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flamarion Prado","family":"Assun\u00e7\u00e3o","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Jos\u00e9 Tena\u00e7ol","family":"Andes","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Andolina","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, 6525 GA Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1607-9246","authenticated-orcid":false,"given":"Vera Margarete","family":"Scarpassa","sequence":"additional","affiliation":[{"name":"Coordena\u00e7\u00e3o de Biodiversidade, Instituto Nacional de Pesquisas da Amaz\u00f4nia, Manaus 69067-375, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3374-9985","authenticated-orcid":false,"given":"Marcus Vin\u00edcius Guimar\u00e3es","family":"de Lacerda","sequence":"additional","affiliation":[{"name":"Instituto de Pesquisa Cl\u00ednica Carlos Borborema (IPCCB), Funda\u00e7\u00e3o de Medicina Tropical Doutor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, AM, Brazil"},{"name":"Instituto Le\u00f4nidas and Maria Deane, Fiocruz Amazonas, Manaus 69057-070, AM, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathryn A.","family":"Hanley","sequence":"additional","affiliation":[{"name":"Department of Biology, New Mexico State University, Las Cruces, NM 88003-8801, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0708-3289","authenticated-orcid":false,"given":"Nikos","family":"Vasilakis","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA"},{"name":"Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA"},{"name":"Institute for Human Infection and Immunity, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA"},{"name":"Center for Tropical Diseases, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA"},{"name":"Center for Vector-Borne and Zoonotic Diseases, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0609, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1126\/science.88.2274.110","article-title":"Yellow fever virus in jungle mosquitoes","volume":"88","author":"Shannon","year":"1938","journal-title":"Science"},{"key":"ref_2","first-page":"1","article-title":"Mosquito studies (Diptera, Culicidae) XXXII: A revision of the genus Haemagogus","volume":"10","author":"Arnell","year":"1973","journal-title":"Contrib. 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