{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:14:06Z","timestamp":1792984446556,"version":"4.5.0"},"reference-count":46,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology of China","award":["2021YFC0863400"],"award-info":[{"award-number":["2021YFC0863400"]}]},{"name":"Ministry of Science and Technology of China","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"Ministry of Science and Technology of China","award":["ANSO-CR-SP-2020-02"],"award-info":[{"award-number":["ANSO-CR-SP-2020-02"]}]},{"name":"Ministry of Science and Technology of China","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"the Alliance of International Scientific Organizations","award":["2021YFC0863400"],"award-info":[{"award-number":["2021YFC0863400"]}]},{"name":"the Alliance of International Scientific Organizations","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"the Alliance of International Scientific Organizations","award":["ANSO-CR-SP-2020-02"],"award-info":[{"award-number":["ANSO-CR-SP-2020-02"]}]},{"name":"the Alliance of International Scientific Organizations","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"Shanghai Municipal Science and Technology Major Project","award":["2021YFC0863400"],"award-info":[{"award-number":["2021YFC0863400"]}]},{"name":"Shanghai Municipal Science and Technology Major Project","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"Shanghai Municipal Science and Technology Major Project","award":["ANSO-CR-SP-2020-02"],"award-info":[{"award-number":["ANSO-CR-SP-2020-02"]}]},{"name":"Shanghai Municipal Science and Technology Major Project","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"Institut Pasteur","award":["2021YFC0863400"],"award-info":[{"award-number":["2021YFC0863400"]}]},{"name":"Institut Pasteur","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"Institut Pasteur","award":["ANSO-CR-SP-2020-02"],"award-info":[{"award-number":["ANSO-CR-SP-2020-02"]}]},{"name":"Institut Pasteur","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]},{"name":"the International Affairs Department of the Institut Pasteur of Paris","award":["2021YFC0863400"],"award-info":[{"award-number":["2021YFC0863400"]}]},{"name":"the International Affairs Department of the Institut Pasteur of Paris","award":["2022YFE0114700"],"award-info":[{"award-number":["2022YFE0114700"]}]},{"name":"the International Affairs Department of the Institut Pasteur of Paris","award":["ANSO-CR-SP-2020-02"],"award-info":[{"award-number":["ANSO-CR-SP-2020-02"]}]},{"name":"the International Affairs Department of the Institut Pasteur of Paris","award":["2019SHZDZX02"],"award-info":[{"award-number":["2019SHZDZX02"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>A recent outbreak of monkeypox virus (mpox) has prompted researchers to explore diagnostics as a means of impeding transmission and further spread. Rapid, sensitive, and specific methods are crucial for accurately diagnosing mpox infections. Here, we developed a loop-mediated isothermal amplification (LAMP) assay for the specific detection of mpox. The primer sets were designed to target regions in and around the N4R gene, and results showed a detection limit of 2 \u00d7 100 DNA copies, which is comparable to the gold-standard qPCR method currently used to detect mpox. Particularly, the assay provides results visible to the naked eye within 30 min. This test specifically detects mpox DNA with no cross-reactivity to related DNA viruses including Varicella Zoster Virus (VZV), Hepatitis B virus (HBV), Vaccinia virus (VACV), Herpes simplex virus-1 (HSV-1), Herpes simplex virus-2 (HSV-2), Human papillomavirus-16 (HPV-16) and Human papillomavirus-18 (HPV-18). Furthermore, the LAMP assay has been evaluated using clinical samples from laboratory-confirmed mpox patients and found to be consistent with the qPCR results. Our results show that this single-tube LAMP method can contribute to diagnosis of suspected mpox infections in the field and clinic, especially in regions with limited laboratory resources.<\/jats:p>","DOI":"10.3390\/v15010084","type":"journal-article","created":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T08:42:22Z","timestamp":1672216942000},"page":"84","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Development of a Novel Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Monkeypox Virus Infections"],"prefix":"10.3390","volume":"15","author":[{"given":"Chao","family":"Yu","sequence":"first","affiliation":[{"name":"Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3975-0453","authenticated-orcid":false,"given":"Lulu","family":"Zuo","sequence":"additional","affiliation":[{"name":"Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China"},{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7481-220X","authenticated-orcid":false,"given":"Jing","family":"Miao","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2451-0569","authenticated-orcid":false,"given":"Lingjing","family":"Mao","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences, Beijing 100049, China"},{"name":"Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Selekon","sequence":"additional","affiliation":[{"name":"Laboratory of Arboviruses, Viral Hemorrhagic Fevers, Emerging viruses and Zoonoses, Institut Pasteur of Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Gonofio","sequence":"additional","affiliation":[{"name":"Laboratory of Arboviruses, Viral Hemorrhagic Fevers, Emerging viruses and Zoonoses, Institut Pasteur of Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Nakoune","sequence":"additional","affiliation":[{"name":"Laboratory of Arboviruses, Viral Hemorrhagic Fevers, Emerging viruses and Zoonoses, Institut Pasteur of Bangui, Bangui P.O. Box 923, Central African Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Berthet","sequence":"additional","affiliation":[{"name":"Centre for Microbes, Development, and Health, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Unit of Discovery and Molecular Characterization of Pathogens, Shanghai 200031, China"},{"name":"Institut Pasteur, Unit\u00e9 Environnement et Risque Infectieux, Cellule d\u2019Intervention Biologique d\u2019Urgence, 75724 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Wong","sequence":"additional","affiliation":[{"name":"Viral Hemorrhagic Fevers Research Unit, CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.3201\/eid2206.150579","article-title":"Extended Human-to-Human Transmission during a Monkeypox Outbreak in the Democratic Republic of the Congo","volume":"22","author":"Nolen","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/S1473-3099(03)00856-9","article-title":"Human monkeypox: An emerging zoonosis","volume":"4","author":"Eckburg","year":"2004","journal-title":"Lancet Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1006\/viro.2002.1446","article-title":"Analysis of the monkeypox virus genome","volume":"297","author":"Shchelkunov","year":"2002","journal-title":"Virology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"306","DOI":"10.15585\/mmwr.mm6710a5","article-title":"Emergence of Monkeypox\u2013West and Central Africa, 1970\u20132017","volume":"67","author":"Durski","year":"2018","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1038\/s41591-022-01907-y","article-title":"Phylogenomic characterization and signs of microevolution in the 2022 multi-country outbreak of monkeypox virus","volume":"28","author":"Isidro","year":"2022","journal-title":"Nat. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"13085","DOI":"10.1038\/s41598-021-92315-8","article-title":"Genomic history of human monkey pox infections in the Central African Republic between 2001 and 2018","volume":"11","author":"Berthet","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Brown, K., and Leggat, P.A. (2016). Human Monkeypox: Current State of Knowledge and Implications for the Future. Trop. Med. Infect. Dis., 1.","DOI":"10.3390\/tropicalmed1010008"},{"key":"ref_8","first-page":"e26756","article-title":"Monkeypox: A Review of Clinical Features, Diagnosis, and Treatment","volume":"14","author":"Cheema","year":"2022","journal-title":"Cureus"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Happi, C., Adetifa, I., Mbala, P., Njouom, R., Nakoune, E., Happi, A., Ndodo, N., Ayansola, O., Mboowa, G., and Bedford, T. (2022). Urgent need for a non-discriminatory and non-stigmatizing nomenclature for monkeypox virus. PLoS Biol., 20.","DOI":"10.1371\/journal.pbio.3001769"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"102387","DOI":"10.1016\/j.tmaid.2022.102387","article-title":"Monkeypox and human transmission: Are we on the verge of another pandemic?","volume":"49","author":"Farahat","year":"2022","journal-title":"Travel Med. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Bunge, E.M., Hoet, B., Chen, L., Lienert, F., Weidenthaler, H., Baer, L.R., and Steffen, R. (2022). The changing epidemiology of human monkeypox-A potential threat? A systematic review. PLoS Negl. Trop. Dis., 16.","DOI":"10.1371\/journal.pntd.0010141"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2200422","DOI":"10.2807\/1560-7917.ES.2022.27.22.2200422","article-title":"Community transmission of monkeypox in the United Kingdom, April to May 2022","volume":"27","author":"Vivancos","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_13","unstructured":"(2022, September 21). Multi-Country Outbreak of Monkeypox, External Situation Report #6\u201321 September 2022. Available online: https:\/\/www.who.int\/publications\/m\/item\/multi-country-outbreak-of-monkeypox--external-situation-report--6---21-september-2022."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1056\/NEJMoa2207323","article-title":"Monkeypox Virus Infection in Humans across 16 Countries\u2013April\u2013June 2022","volume":"387","author":"Thornhill","year":"2022","journal-title":"N. Engl. J. Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1038\/s41579-022-00776-z","article-title":"Monkeypox virus: A neglected zoonotic pathogen spreads globally","volume":"20","author":"Alakunle","year":"2022","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1001\/jama.2022.12513","article-title":"The WHO Declaration of Monkeypox as a Global Public Health Emergency","volume":"328","author":"Nuzzo","year":"2022","journal-title":"JAMA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1093\/ije\/17.3.643","article-title":"The transmission potential of monkeypox virus in human populations","volume":"17","author":"Fine","year":"1988","journal-title":"Int. J. Epidemiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e349","DOI":"10.1016\/S1473-3099(22)00574-6","article-title":"Prevention of monkeypox with vaccines: A rapid review","volume":"22","author":"Poland","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Noe, S., Zange, S., Seilmaier, M., Antwerpen, M.H., Fenzl, T., Schneider, J., Spinner, C.D., Bugert, J.J., Wendtner, C.M., and Wolfel, R. (2022). Clinical and virological features of first human monkeypox cases in Germany. Infection, 1\u20136.","DOI":"10.21203\/rs.3.rs-1725831\/v1"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1093\/jac\/dku545","article-title":"ST-246 is a key antiviral to inhibit the viral F13L phospholipase, one of the essential proteins for orthopoxvirus wrapping","volume":"70","author":"Duraffour","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Andrei, G., Fiten, P., Krecmerova, M., Opdenakker, G., Topalis, D., and Snoeck, R. (2022). Poxviruses Bearing DNA Polymerase Mutations Show Complex Patterns of Cross-Resistance. Biomedicines, 10.","DOI":"10.3390\/biomedicines10030580"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jcv.2006.03.012","article-title":"Detection of monkeypox virus with real-time PCR assays","volume":"36","author":"Li","year":"2006","journal-title":"J. Clin. Virol"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"140","DOI":"10.7883\/yoken.JJID.2008.140","article-title":"Diagnosis and assessment of monkeypox virus (MPXV) infection by quantitative PCR assay: Differentiation of Congo Basin and West African MPXV strains","volume":"61","author":"Saijo","year":"2008","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","article-title":"Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA","volume":"169","author":"Li","year":"2010","journal-title":"J. Virol. Methods"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1016\/j.jmoldx.2022.09.001","article-title":"Validation Study of a Direct Real-Time PCR Protocol for Detection of Monkeypox Virus","volume":"24","author":"Chelsky","year":"2022","journal-title":"J. Mol. Diagn."},{"key":"ref_26","unstructured":"(2022, May 23). Laboratory Testing for the Monkeypox Virus: Interim Guidance. WHO\/MPX\/laboratory\/2022.1. Available online: https:\/\/www.who.int\/publications\/i\/item\/WHO-MPX-laboratory-2022.1."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3109\/10903127.2014.983661","article-title":"Considerations for safe EMS transport of patients infected with Ebola virus","volume":"19","author":"Lowe","year":"2015","journal-title":"Prehosp. Emerg. Care"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"E63","DOI":"10.1093\/nar\/28.12.e63","article-title":"Loop-mediated isothermal amplification of DNA","volume":"28","author":"Notomi","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1111\/jam.13647","article-title":"Loop-mediated isothermal amplification (LAMP): A versatile technique for detection of micro-organisms","volume":"124","author":"Wong","year":"2018","journal-title":"J. Appl. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"113775","DOI":"10.1016\/j.jviromet.2019.113775","article-title":"Development of a real-time loop-mediated isothermal amplification (LAMP) assay and visual LAMP assay for detection of African swine fever virus (ASFV)","volume":"276","author":"Wang","year":"2020","journal-title":"J. Virol. Methods"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"16204","DOI":"10.1021\/acs.analchem.0c04047","article-title":"Isothermal Amplification and Ambient Visualization in a Single Tube for the Detection of SARS-CoV-2 Using Loop-Mediated Amplification and CRISPR Technology","volume":"92","author":"Pang","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.3855\/jidc.11549","article-title":"A quite sensitive fluorescent loop-mediated isothermal amplification for rapid detection of respiratory syncytial virus","volume":"13","author":"Hu","year":"2019","journal-title":"J. Infect. Dev. Ctries"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"eabc7075","DOI":"10.1126\/scitranslmed.abc7075","article-title":"A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples","volume":"12","author":"Herbst","year":"2020","journal-title":"Sci. Transl. Med."},{"key":"ref_34","unstructured":"(2022, June 06). Test Procedure: Monkeypox Virus Generic Real-Time PCR Test, Available online: https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/pdf\/pcr-diagnostic-protocol-508.pdf."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e28036","DOI":"10.1002\/jmv.28036","article-title":"Genomic annotation and molecular evolution of monkeypox virus outbreak in 2022","volume":"95","author":"Wang","year":"2022","journal-title":"J. Med. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Carannante, N., Tiberio, C., Bellopede, R., Liguori, M., Di Martino, F., Maturo, N., Di Sarno, R., Scarica, S., Fusco, G., and Cardillo, L. (2022). Monkeypox Clinical Features and Differential Diagnosis: First Case in Campania Region. Pathogens, 11.","DOI":"10.3390\/pathogens11080869"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5077","DOI":"10.1016\/j.vaccine.2020.04.062","article-title":"Human monkeypox\u2013After 40 years, an unintended consequence of smallpox eradication","volume":"38","author":"Simpson","year":"2020","journal-title":"Vaccine"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.diagmicrobio.2019.03.015","article-title":"Recombinase polymerase amplification assay for rapid detection of Monkeypox virus","volume":"95","author":"Davi","year":"2019","journal-title":"Diagn. Microbiol. Infect. Dis"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mao, L., Ying, J., Selekon, B., Gonofio, E., Wang, X., Nakoune, E., Wong, G., and Berthet, N. (2022). Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA\/RAA) for the Rapid Detection of Monkeypox Virus. Viruses, 14.","DOI":"10.3390\/v14102112"},{"key":"ref_40","first-page":"100120","article-title":"Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens","volume":"3","author":"Garg","year":"2022","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1002\/jmv.21494","article-title":"Loop-mediated isothermal amplification-based diagnostic assay for monkeypox virus infections","volume":"81","author":"Iizuka","year":"2009","journal-title":"J. Med. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e0271422","DOI":"10.1128\/spectrum.02714-22","article-title":"Development of a Loop-Mediated Isothermal Amplification Method for Rapid and Visual Detection of Monkeypox Virus","volume":"10","author":"Feng","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_43","first-page":"2200503","article-title":"Frequent detection of monkeypox virus DNA in saliva, semen, and other clinical samples from 12 patients, Barcelona, Spain, May to June 2022","volume":"27","author":"Fuertes","year":"2022","journal-title":"Eurosurveillance"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"16430","DOI":"10.1038\/s41598-021-95799-6","article-title":"A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples","volume":"11","author":"Amaral","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Li, Z., Bruce, J.L., Cohen, B., Cunningham, C.V., Jack, W.E., Kunin, K., Langhorst, B.W., Miller, J., Moncion, R.A., and Poole, C.B. (2022). Development and implementation of a simple and rapid extraction-free saliva SARS-CoV-2 RT-LAMP workflow for workplace surveillance. PLoS ONE, 17.","DOI":"10.1101\/2022.03.11.22272282"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.jobb.2022.08.001","article-title":"Heat inactivation of monkeypox virus","volume":"4","author":"Grassin","year":"2022","journal-title":"J. Biosaf. Biosecur."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/1\/84\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:53:28Z","timestamp":1760147608000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/1\/84"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,28]]},"references-count":46,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023,1]]}},"alternative-id":["v15010084"],"URL":"https:\/\/doi.org\/10.3390\/v15010084","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,28]]}}}