{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:16:45Z","timestamp":1791505005933,"version":"4.3.4"},"reference-count":36,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Agriculture, Forestry, and the Fisheries of Japan","award":["JPJ008617.18065101"],"award-info":[{"award-number":["JPJ008617.18065101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>H5N8 and H5N1 high pathogenicity avian influenza viruses (HPAIVs) caused outbreaks in poultry farms in Japan from November 2021 to May 2022. Hemagglutinin genes of these viruses belong to clade 2.3.4.4B and can be divided phylogenetically into the following groups: 20A, 20E, and 21E. In this study, we compared the infectivity and transmissibility of HPAIVs from three groups of chickens. Representative strains from 20A, 20E, and 21E groups are A\/chicken\/Akita\/7C\/2021(H5N8)(Akita7C), A\/chicken\/Kagoshima\/21A6T\/2021(H5N1)(Kagoshima6T), and A\/chicken\/Iwate\/21A7T\/2022(H5N1)(Iwate7T), respectively. Fifty percent lethal dose of Akita7C in chickens (103.83 fifty percent egg infectious dose (EID50)) was up to seven times lower than those of Kagoshima6T and Iwate7T (104.50 and 104.68 EID50, respectively). Mean death times for Akita7C- and Kagoshima6T-infected chickens (3.45 and 3.30 days, respectively) were at least a day longer than that of Iwate7T (2.20 days). Viral titers of the trachea and cloaca of Iwate7T-infected chicken were the highest detected. The transmission rate of the Akita7C strain (100%) was markedly higher than those of the two strains (&lt;50%). These data suggest that the infectivity and transmissibility of the Akita7C strain (H5N8) in chickens are higher than those of H5N1 viruses, providing fundamental information needed for formulating effective prevention and control strategies for HPAI outbreaks.<\/jats:p>","DOI":"10.3390\/v15020265","type":"journal-article","created":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T05:40:02Z","timestamp":1673934002000},"page":"265","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Different Infectivity and Transmissibility of H5N8 and H5N1 High Pathogenicity Avian Influenza Viruses Isolated from Chickens in Japan in the 2021\/2022 Season"],"prefix":"10.3390","volume":"15","author":[{"given":"Yoshihiro","family":"Takadate","sequence":"first","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryota","family":"Tsunekuni","sequence":"additional","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuka","family":"Kumagai","sequence":"additional","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1603-8471","authenticated-orcid":false,"given":"Junki","family":"Mine","sequence":"additional","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuto","family":"Kikutani","sequence":"additional","affiliation":[{"name":"National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, Tokyo 1858511, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saki","family":"Sakuma","sequence":"additional","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0318-7275","authenticated-orcid":false,"given":"Kohtaro","family":"Miyazawa","sequence":"additional","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Uchida","sequence":"additional","affiliation":[{"name":"Emerging Virus Group, Division of Zoonosis Research, National Institute of Animal Health, National Agriculture and Food Research Organization, Ibaraki 305856, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1006\/viro.1999.9820","article-title":"Genetic characterization of the pathogenic influenza A\/Goose\/Guangdong\/1\/96 (H5N1) virus: Similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong","volume":"261","author":"Xu","year":"1999","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"20180257","DOI":"10.1098\/rstb.2018.0257","article-title":"A brief history of bird flu","volume":"374","author":"Lycett","year":"2019","journal-title":"Philos. 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