{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:44:25Z","timestamp":1791333865791,"version":"4.3.1"},"reference-count":71,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,28]],"date-time":"2023-01-28T00:00:00Z","timestamp":1674864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"key project of Jiangsu Province\u2019s Key Research and Development Plan (modern Agriculture)","award":["BE2020407"],"award-info":[{"award-number":["BE2020407"]}]},{"name":"key project of Jiangsu Province\u2019s Key Research and Development Plan (modern Agriculture)","award":["NSF2022CB04"],"award-info":[{"award-number":["NSF2022CB04"]}]},{"name":"key project of Jiangsu Province\u2019s Key Research and Development Plan (modern Agriculture)","award":["NSF2022CB25"],"award-info":[{"award-number":["NSF2022CB25"]}]},{"name":"key project of Jiangsu Province\u2019s Key Research and Development Plan (modern Agriculture)","award":["2020220375"],"award-info":[{"award-number":["2020220375"]}]},{"name":"project of Jiangsu Agri-animal Husbandry Vocational College","award":["BE2020407"],"award-info":[{"award-number":["BE2020407"]}]},{"name":"project of Jiangsu Agri-animal Husbandry Vocational College","award":["NSF2022CB04"],"award-info":[{"award-number":["NSF2022CB04"]}]},{"name":"project of Jiangsu Agri-animal Husbandry Vocational College","award":["NSF2022CB25"],"award-info":[{"award-number":["NSF2022CB25"]}]},{"name":"project of Jiangsu Agri-animal Husbandry Vocational College","award":["2020220375"],"award-info":[{"award-number":["2020220375"]}]},{"name":"Natural Science Research Project of Higher Education of Jiangsu Province","award":["BE2020407"],"award-info":[{"award-number":["BE2020407"]}]},{"name":"Natural Science Research Project of Higher Education of Jiangsu Province","award":["NSF2022CB04"],"award-info":[{"award-number":["NSF2022CB04"]}]},{"name":"Natural Science Research Project of Higher Education of Jiangsu Province","award":["NSF2022CB25"],"award-info":[{"award-number":["NSF2022CB25"]}]},{"name":"Natural Science Research Project of Higher Education of Jiangsu Province","award":["2020220375"],"award-info":[{"award-number":["2020220375"]}]},{"name":"Qing Lan Project of Jiangsu Province","award":["BE2020407"],"award-info":[{"award-number":["BE2020407"]}]},{"name":"Qing Lan Project of Jiangsu Province","award":["NSF2022CB04"],"award-info":[{"award-number":["NSF2022CB04"]}]},{"name":"Qing Lan Project of Jiangsu Province","award":["NSF2022CB25"],"award-info":[{"award-number":["NSF2022CB25"]}]},{"name":"Qing Lan Project of Jiangsu Province","award":["2020220375"],"award-info":[{"award-number":["2020220375"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Pseudorabies virus (PRV) is the pathogen of pseudorabies (PR), which belongs to the alpha herpesvirus subfamily with a double stranded DNA genome encoding approximately 70 proteins. PRV has many non-essential regions for replication, has a strong capacity to accommodate foreign genes, and more areas for genetic modification. PRV is an ideal vaccine vector, and multivalent live virus-vectored vaccines can be developed using the gene-deleted PRV. The immune system continues to be stimulated by the gene-deleted PRVs and maintain a long immunity lasting more than 4 months. Here, we provide a brief overview of the biology of PRV, recombinant PRV construction methodology, the technology platform for efficiently constructing recombinant PRV, and the applications of recombinant PRV in vaccine development. This review summarizes the latest information on PRV usage in vaccine development against swine infectious diseases, and it offers novel perspectives for advancing preventive medicine through vaccinology.<\/jats:p>","DOI":"10.3390\/v15020370","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T07:34:41Z","timestamp":1675064081000},"page":"370","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Recombinant Pseudorabies Virus Usage in Vaccine Development against Swine Infectious Disease"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1135-1848","authenticated-orcid":false,"given":"Mo","family":"Zhou","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4333-6423","authenticated-orcid":false,"given":"Muhammad","family":"Abid","sequence":"additional","affiliation":[{"name":"Viral Oncogenesis Group, The Pirbright Institute, Ash Road Pirbright, Woking, Surrey GU24 0NF, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinuo","family":"Cao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanyuan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Engineering Technology Research Center for Modern Animal Science and Novel Veterinary Pharmaceutic Development, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225306, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"5733","DOI":"10.1016\/j.vaccine.2015.09.066","article-title":"Novel pseudorabies virus variant with defects in TK, gE and gI protects growing pigs against lethal challenge","volume":"33","author":"Hu","year":"2015","journal-title":"Vaccine"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zhao, X., Tong, W., Song, X., Jia, R., Li, L., Zou, Y., He, C., Liang, X., Lv, C., and Jing, B. 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