{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:11:24Z","timestamp":1790759484400,"version":"4.1.0"},"reference-count":65,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T00:00:00Z","timestamp":1675641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agency for Nature and Forests"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Tick-borne encephalitis virus (TBEV) is a flavivirus transmitted by ticks. Serological screenings in animals are performed to estimate the prevalence and distribution of TBEV. Most screenings consist of a primary screening by ELISA, followed by confirmation of positive samples by plaque reduction neutralization tests (PRNTs). In this study, 406 wild boar sera were tested with 2 regularly used commercial ELISAs for flavivirus screening in animals (Immunozym FSME (TBEV) IgG All Species (Progen) and ID Screen West Nile Competition (Innovative Diagnostics)) and PRNTs for TBEV and USUTU virus. The results showed that the Immunozym and IDScreen ELISAs had low relative sensitivities of 23% and 20%, respectively, compared to the PRNT results. The relative specificities were 88% and 84% due to cross reactions with USUTU virus-specific antibodies. The minimal TBEV prevalence in our sample set was 8.6% when determined by PRNT. When the screening approach of ELISA testing followed by PRNT confirmation was applied, a TBEV seroprevalence of only 2.0% and 1.7% was found. The suboptimal performance of the ELISAs was confirmed by testing sera collected from experimentally TBEV-infected sheep. While the PRNT detected TBEV specific antibodies in 94% of samples collected between 7 and 18 days post-infection, the ELISAs classified only 50% and 31% of the samples as positive. Both routinely used ELISAs for TBEV antibody screening in animal sera were shown to have a low sensitivity, potentially leading to an underestimation of the true prevalence, and furthermore cross-react with other flavivirus antibodies.<\/jats:p>","DOI":"10.3390\/v15020459","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T02:28:17Z","timestamp":1675736897000},"page":"459","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of Serological Methods for Tick-Borne Encephalitis Virus-Specific Antibody Detection in Wild Boar and Sheep: Impact of the Screening Approach on the Estimated Seroprevalence"],"prefix":"10.3390","volume":"15","author":[{"given":"Gabrielle","family":"Trozzi","sequence":"first","affiliation":[{"name":"Unit of Exotic and Vector-Borne Diseases, Sciensano, Groeselenberg 99, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadjah Radia","family":"Adjadj","sequence":"additional","affiliation":[{"name":"Unit of Exotic and Vector-Borne Diseases, Sciensano, Groeselenberg 99, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6131-2831","authenticated-orcid":false,"given":"Muriel","family":"Vervaeke","sequence":"additional","affiliation":[{"name":"Agency for Nature and Forests, 1000 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Severine","family":"Matthijs","sequence":"additional","affiliation":[{"name":"Viral Reemerging, Enzootic and Bee Diseases, Sciensano, Groeselenberg 99, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Sohier","sequence":"additional","affiliation":[{"name":"Unit of Exotic and Vector-Borne Diseases, Sciensano, Groeselenberg 99, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick","family":"De Regge","sequence":"additional","affiliation":[{"name":"Unit of Exotic and Vector-Borne Diseases, Sciensano, Groeselenberg 99, 1180 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1099\/0022-1317-78-5-1049","article-title":"Infectious CDNA Clones of Tick-Borne Encephalitis Virus European Subtype Prototypic Strain Neudoerfl and High Virulence Strain Hypr","volume":"78","author":"Mandl","year":"1997","journal-title":"J. Gen. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"430","DOI":"10.12998\/wjcc.v3.i5.430","article-title":"Tick-Borne Encephalitis: A Review of Epidemiology, Clinical Characteristics, and Management","volume":"3","author":"Bogovic","year":"2015","journal-title":"WJCC"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"veac051","DOI":"10.1093\/ve\/veac051","article-title":"Evolutionary Traits of Tick-Borne Encephalitis Virus: Pervasive Non-Coding RNA Structure Conservation and Molecular Epidemiology","volume":"8","author":"Kutschera","year":"2022","journal-title":"Virus Evol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1099\/vir.0.011437-0","article-title":"Tick-Borne Encephalitis Virus\u2014A Review of an Emerging Zoonosis","volume":"90","author":"Mansfield","year":"2009","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1017\/S0031182014001486","article-title":"Co-Feeding Transmission in Lyme Disease Pathogens","volume":"142","author":"Voordouw","year":"2015","journal-title":"Parasitology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1292\/jvms.18-0373","article-title":"Epidemiology and Pathological Mechanisms of Tick-Borne Encephalitis","volume":"81","author":"Yoshii","year":"2019","journal-title":"J. Vet. Med. Sci."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"S19","DOI":"10.1016\/S0264-410X(02)00812-5","article-title":"Epidemiology and Ecology of TBE Relevant to the Production of Effective Vaccines","volume":"21","year":"2003","journal-title":"Vaccine"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.idc.2008.03.013","article-title":"Tick-Borne Encephalitis","volume":"22","author":"Kaiser","year":"2008","journal-title":"Infect. Dis. Clin. North Am."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Bournez, L., Umhang, G., Moinet, M., Boucher, J.-M., Demerson, J.-M., Caillot, C., Legras, L., Devillers, E., Hansmann, Y., and Velay, A. (2020). Disappearance of TBEV Circulation among Rodents in a Natural Focus in Alsace, Eastern France. Pathogens, 9.","DOI":"10.3390\/pathogens9110930"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13071-019-3806-z","article-title":"Prevalence of Pathogens in Ticks Collected from Humans through Citizen Science in Belgium","volume":"12","author":"Lernout","year":"2019","journal-title":"Parasites Vectors"},{"key":"ref_11","first-page":"31099","article-title":"First TBEV Serological Screening in Flemish Wild Boar","volume":"6","author":"Roelandt","year":"2016","journal-title":"Infect. Ecol. Epidemiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1089\/vbz.2011.0647","article-title":"Tick-Borne Encephalitis Virus Seropositive Dog Detected in Belgium: Screening of the Canine Population as Sentinels for Public Health","volume":"11","author":"Roelandt","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1089\/vbz.2014.1576","article-title":"Autochthonous Tick-Borne Encephalitis Virus-Seropositive Cattle in Belgium: A Risk-Based Targeted Serological Survey","volume":"14","author":"Roelandt","year":"2014","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1136\/vr.e646","article-title":"Tickborne Encephalitis Virus Antibodies in Wild Cervids in Belgium","volume":"170","author":"Linden","year":"2012","journal-title":"Vet. Rec."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Adjadj, N.R., Vervaeke, M., Sohier, C., Cargnel, M., and De Regge, N. (2022). Tick-Borne Encephalitis Virus Prevalence in Sheep, Wild Boar and Ticks in Belgium. Viruses, 14.","DOI":"10.3390\/v14112362"},{"key":"ref_16","first-page":"16","article-title":"Comparative Tick-Borne Encephalitis (Virus) Surveillance in Belgium 2009\u20132015: Experiences with Diagnostic Tests, Sentinel Species and Surveillance Designs","volume":"1","author":"Roelandt","year":"2017","journal-title":"J. Zoonotic Dis. Public Health"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.3201\/eid2708.211175","article-title":"Autochthonous Cases of Tick-Borne Encephalitis, Belgium, 2020","volume":"27","author":"Stoefs","year":"2021","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1186\/s13071-016-1335-6","article-title":"Serological Evidence of Tick-Borne Encephalitis Virus Infection in Moose and Deer in Finland: Sentinels for Virus Circulation","volume":"9","author":"Tonteri","year":"2016","journal-title":"Parasites Vectors"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.ttbdis.2011.09.011","article-title":"Goats and Sheep as Sentinels for Tick-Borne Encephalitis (TBE) Virus\u2014Epidemiological Studies in Areas Endemic and Non-Endemic for TBE Virus in Germany","volume":"3","author":"Klaus","year":"2012","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Haut, M., Girl, P., Oswald, B., Romig, T., Obiegala, A., Dobler, G., and Pfeffer, M. (2020). The Red Fox (Vulpes Vulpes) as Sentinel for Tick-Borne Encephalitis Virus in Endemic and Non-Endemic Areas. Microorganisms, 8.","DOI":"10.3390\/microorganisms8111817"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.1016\/j.ttbdis.2016.08.004","article-title":"Seroprevalence of Tick-Borne-Encephalitis Virus in Wild Game in Mecklenburg-Western Pomerania (North-Eastern Germany)","volume":"7","author":"Frimmel","year":"2016","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/zph.12675","article-title":"Cervids as Sentinel-species for Tick-borne Encephalitis Virus in Norway\u2014A Serological Study","volume":"67","author":"Paulsen","year":"2020","journal-title":"Zoonoses Public Health"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"101799","DOI":"10.1016\/j.ttbdis.2021.101799","article-title":"The European Bison (Bison Bonasus) as an Indicatory Species for the Circulation of Tick-Borne Encephalitis Virus (TBEV) in Natural Foci in Poland","volume":"12","author":"Krzysiak","year":"2021","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Grzybek, M., Alsarraf, M., To\u0142kacz, K., Behnke-Borowczyk, J., Biernat, B., Sta\u0144czak, J., Strachecka, A., Guz, L., Szczepaniak, K., and Paleolog, J. (2018). Seroprevalence of TBEV in Bank Voles from Poland\u2014A Long-Term Approach. Emerg. Microbes Infect., 7.","DOI":"10.1038\/s41426-018-0149-3"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"492","DOI":"10.7589\/2019-02-048","article-title":"Seroprevalence of Tick-Borne Encephalitis Virus in Three Species of Voles (Microtus Spp.) in Poland","volume":"56","author":"Grzybek","year":"2020","journal-title":"J. Wildl. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.ttbdis.2014.06.007","article-title":"Prevalence of Antibodies against Tick-Borne Encephalitis Virus in Wild Game from Saxony, Germany","volume":"5","author":"Balling","year":"2014","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1900658","DOI":"10.2807\/1560-7917.ES.2019.24.47.1900658","article-title":"Detection of New Endemic Focus of Tick-Borne Encephalitis Virus (TBEV), Hampshire\/Dorset Border, England, September 2019","volume":"24","author":"Holding","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1186\/s13028-021-00597-9","article-title":"Evaluation of Antibodies in Cerebrospinal Fluid for the Diagnosis of Tick-Borne Encephalitis in Dogs","volume":"63","author":"Alnefelt","year":"2021","journal-title":"Acta Vet. Scand."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ttbdis.2010.06.001","article-title":"Seroprevalence of Tick-Borne Encephalitis (TBE) in Naturally Exposed Monkeys (Macaca Sylvanus) and Sheep and Prevalence of TBE Virus in Ticks in a TBE Endemic Area in Germany","volume":"1","author":"Klaus","year":"2010","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1016\/j.ttbdis.2019.04.006","article-title":"First Detection of TBE Virus in Ticks and Sero-Reactivity in Goats in a Non-Endemic Region in the Southern Part of Switzerland (Canton of Ticino)","volume":"10","author":"Klaus","year":"2019","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.vetmic.2012.12.041","article-title":"Tick-Borne Encephalitis Virus (TBEV) Infection in Horses: Clinical and Laboratory Findings and Epidemiological Investigations","volume":"163","author":"Klaus","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/j.ttbdis.2014.03.008","article-title":"Seroprevalence of Borrelia Burgdorferi Sensu Lato and Tick-Borne Encephalitis Virus in Zoo Animal Species in the Czech Republic","volume":"5","author":"Golovchenko","year":"2014","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Khamassi Khbou, M., Romdhane, R., Foughali, A.A., Sassi, L., Suin, V., Rekik, M., and Benzarti, M. (2020). Presence of Antibodies against Tick-Borne Encephalitis Virus in Sheep in Tunisia, North Africa. BMC Vet Res, 16.","DOI":"10.1186\/s12917-020-02651-6"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/j.ttbdis.2016.05.002","article-title":"Prevalence Estimation of Tick-Borne Encephalitis Virus (TBEV) Antibodies in Dogs from Finland Using Novel Dog Anti-TBEV IgG MAb-Capture and IgG Immunofluorescence Assays Based on Recombinant TBEV Subviral Particles","volume":"7","author":"Levanov","year":"2016","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Girl, P., Haut, M., Riederer, S., Pfeffer, M., and Dobler, G. (2021). Comparison of Three Serological Methods for the Epidemiological Investigation of TBE in Dogs. Microorganisms, 9.","DOI":"10.3390\/microorganisms9020399"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1089\/vbz.2012.1236","article-title":"Demonstration of Usutu Virus Antibodies in Horses, Croatia","volume":"13","author":"Barbic","year":"2013","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"101487","DOI":"10.1016\/j.ttbdis.2020.101487","article-title":"Detection of Antibodies against Tick-Borne Encephalitis Flaviviruses in Breeding and Sport Horses from Spain","volume":"11","author":"Camino","year":"2020","journal-title":"Ticks Tick-Borne Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1017\/S0950268814002878","article-title":"High Prevalence of West Nile Virus in Equines from the Two Provinces of Pakistan","volume":"143","author":"Zohaib","year":"2015","journal-title":"Epidemiol. Infect."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1089\/vbz.2017.2167","article-title":"Molecular Detection and Serological Evidence of Tick-Borne Encephalitis Virus in Serbia","volume":"17","author":"Potkonjak","year":"2017","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Beck, C., Leparc-Goffart, I., Desoutter, D., Deberg\u00e9, E., Bichet, H., Lowenski, S., Dumarest, M., Gonzalez, G., Mign\u00e9, C., and Vanhomwegen, J. (2019). Serological Evidence of Infection with Dengue and Zika Viruses in Horses on French Pacific Islands. PLoS Negl Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0007162"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Petruccelli, A., Zottola, T., Ferrara, G., Iovane, V., Di Russo, C., Pagnini, U., and Montagnaro, S. (2020). West Nile Virus and Related Flavivirus in European Wild Boar (Sus Scrofa), Latium Region, Italy: A Retrospective Study. Animals, 10.","DOI":"10.3390\/ani10030494"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.3390\/ijerph10083112","article-title":"Use of Competition ELISA for Monitoring of West Nile Virus Infections in Horses in Germany","volume":"10","author":"Ziegler","year":"2013","journal-title":"IJERPH"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1089\/vbz.2016.2042","article-title":"Circulation of Zoonotic Arboviruses in Equine Populations of Mallorca Island (Spain)","volume":"17","author":"Vanhomwegen","year":"2017","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1089\/vbz.2017.2216","article-title":"A Serosurvey of Flavivirus Infection in Horses and Birds in Slovakia","volume":"18","author":"Csank","year":"2018","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/j.ijpara.2019.05.006","article-title":"Changes in Host Densities and Co-Feeding Pattern Efficiently Predict Tick-Borne Encephalitis Hazard in an Endemic Focus in Northern Italy","volume":"49","author":"Tagliapietra","year":"2019","journal-title":"Int. J. Parasitol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"108763","DOI":"10.1016\/j.vetmic.2020.108763","article-title":"Evidence of Exposure to Zoonotic Flaviviruses in Zoo Mammals in Spain and Their Potential Role as Sentinel Species","volume":"247","author":"Lecollinet","year":"2020","journal-title":"Vet. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/tbed.12801","article-title":"Exposure to West Nile Virus and Tick-Borne Encephalitis Virus in Dogs in Spain","volume":"65","author":"Lecollinet","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Rieille, N., Klaus, C., Hoffmann, D., P\u00e9ter, O., and Voordouw, M.J. (2017). Goats as Sentinel Hosts for the Detection of Tick-Borne Encephalitis Risk Areas in the Canton of Valais, Switzerland. BMC Vet. Res., 13.","DOI":"10.1186\/s12917-017-1136-y"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1002\/vms3.342","article-title":"Serological Evidence of West Nile Virus Infection among Birds and Horses in Some Geographical Locations of Iran","volume":"7","author":"Bakhshi","year":"2021","journal-title":"Vet Med. Sci."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Pautienius, A., Armonaite, A., Simkute, E., Zagrabskaite, R., Buitkuviene, J., Alpizar-Jara, R., Grigas, J., Zakiene, I., Zienius, D., and Salomskas, A. (2021). Cross-Sectional Study on the Prevalence and Factors Influencing Occurrence of Tick-Borne Encephalitis in Horses in Lithuania. Pathogens, 10.","DOI":"10.3390\/pathogens10020140"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S1386-6532(00)00178-5","article-title":"Comparison of Six Different Commercial IgG-ELISA Kits for the Detection of TBEV-Antibodies","volume":"20","author":"Niedrig","year":"2001","journal-title":"J. Clin. Virol."},{"key":"ref_52","unstructured":"Van Maanen, K., Reusken, C., Reimerink, J., and Van der Heejden, H. (2010). Surveillance of West Nile Virus Incursions in the Netherlands: Validation of Antibody Detecting ELISAs in Horses, Epizone."},{"key":"ref_53","unstructured":"Diaz Sanchez, S., H\u00f6fle, U., Blanco, J.M., Jim\u00e9nez-Clavero, M.A., Llorente, F., Rodriguez, V., and Perez-Ramirez, E. (2009). Use of a Multispecies Competitive ELISA for Investigation of Exposure to West Nile Virus in Multiple Species of Captive and Wild Birds, WAVLD."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1089\/vbz.2009.0237","article-title":"Serosurvey of West Nile Virus and Other Flaviviruses of the Japanese Encephalitis Antigenic Complex in Birds from Andalusia, Southern Spain","volume":"11","author":"Busquets","year":"2011","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.4049\/jimmunol.98.6.1136","article-title":"The Effect of Kaolin on Immunoglobulins: An Improved Technique to Remove the Nonspecific Serum Inhibitor of Reovirus Hemagglutination","volume":"98","author":"Mann","year":"1967","journal-title":"J. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Beck, C., Despr\u00e8s, P., Paulous, S., Vanhomwegen, J., Lowenski, S., Nowotny, N., Durand, B., Garnier, A., Blaise-Boisseau, S., and Guitton, E. (2015). A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. BioMed Res. Int., 2015.","DOI":"10.1155\/2015\/678084"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"363","DOI":"10.2307\/2529786","article-title":"An Application of Hierarchical Kappa-Type Statistics in the Assessment of Majority Agreement among Multiple Observers","volume":"33","author":"Landis","year":"1977","journal-title":"Biometrics"},{"key":"ref_58","first-page":"443","article-title":"Evaluation of serological tests for detecting tick-borne encephalitis virus (TBEV) antibodies in animals","volume":"124","author":"Klaus","year":"2011","journal-title":"Berl. Munch. Tierarztl. Wochenschr."},{"key":"ref_59","unstructured":"Schmidt, N., and Lennette, E.H. (1965). Viral and Rickettsial Infections of Man, Lippincott Co."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"748","DOI":"10.1128\/am.20.5.748-753.1970","article-title":"Comparison of Methods for Removal of Nonspecific Inhibitors of Arbovirus Hemagglutination","volume":"20","author":"Monath","year":"1970","journal-title":"Appl. Microbiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1007\/s00508-004-0191-0","article-title":"High Seroprevalence of Granulocytic Ehrlichiosis Distinguishes Sheep That Were the Source of an Alimentary Epidemic of Tick-Borne Encephalitis","volume":"116","author":"Zeman","year":"2004","journal-title":"Wien. Klin. Wochenschr."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/j.vetmic.2008.09.082","article-title":"Serological Survey of Domestic Animals for Tick-Borne Encephalitis and Bhanja Viruses in Northeastern Hungary","volume":"135","author":"Hornok","year":"2009","journal-title":"Vet. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1089\/vbz.2019.2469","article-title":"Usutu Virus Epizootic in Belgium in 2017 and 2018: Evidence of Virus Endemization and Ongoing Introduction Events","volume":"20","author":"Benzarti","year":"2020","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Vilibic-Cavlek, T., Petrovi\u0107, T., Barbic, L., Listes, E., and Savini, G. (2020). Epidemiology of Usutu Virus: The European Scenario. Pathogens, 9.","DOI":"10.3390\/pathogens9090699"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.1111\/tbed.12569","article-title":"A Serological Protein Microarray for Detection of Multiple Cross-Reactive Flavivirus Infections in Horses for Veterinary and Public Health Surveillance","volume":"64","author":"Cleton","year":"2017","journal-title":"Transbound. Emerg. Dis."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/2\/459\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:26:06Z","timestamp":1760120766000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/2\/459"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,6]]},"references-count":65,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["v15020459"],"URL":"https:\/\/doi.org\/10.3390\/v15020459","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,6]]}}}