{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:21:41Z","timestamp":1792513301715,"version":"4.5.0"},"reference-count":67,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T00:00:00Z","timestamp":1675728000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Department of Orthopaedics at the University of Utah"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Staphylococcus aureus causes the majority of implant-related infections. These infections present as biofilms, in which bacteria adhere to the surface of foreign materials and form robust communities that are resilient to the human immune system and antibiotic drugs. The heavy use of broad-spectrum antibiotics against these pathogens disturbs the host\u2019s microbiome and contributes to the growing problem of antibiotic-resistant infections. The use of bacteriophages as antibacterial agents is a potential alternative therapy. In this study, bioluminescent strains of S. aureus were grown to form 48-h biofilms on polyether ether ketone (PEEK), a material used to manufacture orthopaedic implants, in either static or dynamic growth conditions. Biofilms were treated with vancomycin, staphylococcal phage, or a combination of the two. We showed that vancomycin and staph phages were able to independently reduce the total bacterial load. Most phage-antibiotic combinations produced greater log reductions in surviving bacteria compared to single-agent treatments, suggesting antimicrobial synergism. In addition to demonstrating the efficacy of combining vancomycin and staph phage, our results demonstrate the importance of growth conditions in phage-antibiotic combination studies. Dynamic biofilms were found to have a substantial impact on apparent treatment efficacy, as they were more resilient to combination treatments than static biofilms.<\/jats:p>","DOI":"10.3390\/v15020460","type":"journal-article","created":{"date-parts":[[2023,2,7]],"date-time":"2023-02-07T02:28:17Z","timestamp":1675736897000},"page":"460","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Phage and Antibiotic Combinations Reduce Staphylococcus aureus in Static and Dynamic Biofilms Grown on an Implant Material"],"prefix":"10.3390","volume":"15","author":[{"given":"Hyonoo","family":"Joo","sequence":"first","affiliation":[{"name":"Micro-Phage Laboratory, Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA"},{"name":"Department of Veterans Affairs, Salt Lake City Health Care System, Salt Lake City, UT 84148, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijia M.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Veterans Affairs, Salt Lake City Health Care System, Salt Lake City, UT 84148, USA"},{"name":"Department of Orthopaedics, University of Utah, Salt Lake City, UT 84108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isha","family":"Soni","sequence":"additional","affiliation":[{"name":"Micro-Phage Laboratory, Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA"},{"name":"Department of Orthopaedics, University of Utah, Salt Lake City, UT 84108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Wang-Crocker","sequence":"additional","affiliation":[{"name":"Micro-Phage Laboratory, Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA"},{"name":"Department of Orthopaedics, University of Utah, Salt Lake City, UT 84108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tyson","family":"Matern","sequence":"additional","affiliation":[{"name":"Micro-Phage Laboratory, Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA"},{"name":"Department of Orthopaedics, University of Utah, Salt Lake City, UT 84108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James Peter","family":"Beck","sequence":"additional","affiliation":[{"name":"Department of Veterans Affairs, Salt Lake City Health Care System, Salt Lake City, UT 84148, USA"},{"name":"Department of Orthopaedics, University of Utah, Salt Lake City, UT 84108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Loc-Carrillo","sequence":"additional","affiliation":[{"name":"Micro-Phage Laboratory, Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA"},{"name":"Department of Veterans Affairs, Salt Lake City Health Care System, Salt Lake City, UT 84148, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.12968\/jowc.2010.19.2.46966","article-title":"Chronic Wounds and the Medical Biofilm Paradigm","volume":"19","author":"Wolcott","year":"2010","journal-title":"J. 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