{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T02:35:01Z","timestamp":1792290901028,"version":"4.4.0"},"reference-count":60,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82161138001"],"award-info":[{"award-number":["82161138001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022YFC2602202"],"award-info":[{"award-number":["2022YFC2602202"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["82161138001"],"award-info":[{"award-number":["82161138001"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"National Key R&amp;D Program of China","doi-asserted-by":"publisher","award":["2022YFC2602202"],"award-info":[{"award-number":["2022YFC2602202"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The transcriptome of fowl adenovirus has not been comprehensively revealed. Here, we attempted to analyze the fowl adenovirus 4 (FAdV-4) transcriptome by deep sequencing. RNA samples were extracted from chicken LMH cells at 12, 18 or 26 h post-FAdV-4 infection, and subjected to Illumina strand-specific RNA-seq or nanopore full-length PCR-cDNA sequencing. After removing the reads of host cells, the data of FAdV-4 nanopore full-length cDNAs (transcripts) were corrected with reads from the Illumina RNA-seq, mapped to the viral genome and then used to predict viral open reading frames (ORFs). Other than 42 known ORFs, 39 novel ORFs were annotated to the FAdV-4 genome. Different from human adenovirus 5, one FAdV-4 ORF was often encoded by several transcripts, and more FAdV-4 ORFs were located on two exons. With these data, 18 major transcription start sites and 15 major transcription termination sites were defined, implying 18 viral promoters and 15 polyadenylation signals. The temporal cascade of viral gene transcription was observed in FAdV-4-infected cells, with six promoters possessing considerable activity in the early phase. Unexpectedly, four promoters, instead of one major late promoter, were engaged in the transcription of the viral genus-common genes on the forward strand. The clarification of the FAdV-4 transcriptome laid a solid foundation for the study of viral gene function, virulence and virus evolution, and it would help construct FAdV-4 as a gene transfer vehicle. The strategy of de novo ORF prediction could be used to parse the transcriptome of other novel adenoviruses.<\/jats:p>","DOI":"10.3390\/v15020529","type":"journal-article","created":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T03:09:21Z","timestamp":1676430561000},"page":"529","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of Fowl Adenovirus 4 Transcriptome by De Novo ORF Prediction Based on Corrected Nanopore Full-Length cDNA Sequencing Data"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5767-401X","authenticated-orcid":false,"given":"Zhuozhuang","family":"Lu","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjin","family":"Wang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6834-1341","authenticated-orcid":false,"given":"Xiaohui","family":"Zou","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Hung","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1721","DOI":"10.1099\/jgv.0.001721","article-title":"ICTV Virus Taxonomy Profile: Adenoviridae 2022","volume":"103","author":"Benko","year":"2022","journal-title":"J. Gen. Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"570","DOI":"10.3181\/00379727-84-20714","article-title":"Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture","volume":"84","author":"Rowe","year":"1953","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1016\/0092-8674(80)90362-1","article-title":"DNA replication and the early to late transition in adenovirus infection","volume":"22","author":"Thomas","year":"1980","journal-title":"Cell"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Price, A.M., Steinbock, R.T., Lauman, R., Charman, M., Hayer, K.E., Kumar, N., Halko, E., Lum, K.K., Wei, M., and Wilson, A.C. (2022). Novel viral splicing events and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts. PLoS Pathog., 18.","DOI":"10.1371\/journal.ppat.1010797"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Watanabe, M., Nishikawaji, Y., Kawakami, H., and Kosai, K.I. (2021). Adenovirus Biology, Recombinant Adenovirus, and Adenovirus Usage in Gene Therapy. Viruses, 13.","DOI":"10.3390\/v13122502"},{"key":"ref_6","first-page":"213","article-title":"The structure and function of the adenovirus major late promoter","volume":"272","author":"Young","year":"2003","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1016\/0092-8674(80)90568-1","article-title":"Transcripts from the adenovirus-2 major late promoter yield a single early family of 3\u2032 coterminal mRNAs and five late families","volume":"22","author":"Shaw","year":"1980","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/0092-8674(77)90294-X","article-title":"A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids","volume":"11","author":"Chow","year":"1977","journal-title":"Cell"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/0022-2836(79)90036-6","article-title":"Complex splicing patterns of RNAs from the early regions of adenovirus-2","volume":"134","author":"Chow","year":"1979","journal-title":"J. Mol. Biol."},{"key":"ref_10","first-page":"e01869-20","article-title":"The Human Adenovirus Type 2 Transcriptome: An Amazing Complexity of Alternatively Spliced mRNAs","volume":"95","author":"Segerman","year":"2020","journal-title":"J. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"12918","DOI":"10.1128\/JVI.01531-07","article-title":"Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit","volume":"81","author":"Tollefson","year":"2007","journal-title":"J. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7096","DOI":"10.1128\/JVI.00107-10","article-title":"Adenovirus late-phase infection is controlled by a novel L4 promoter","volume":"84","author":"Morris","year":"2010","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1038\/s42003-020-0849-9","article-title":"Deep splicing plasticity of the human adenovirus type 5 transcriptome drives virus evolution","volume":"3","author":"Turnell","year":"2020","journal-title":"Commun. Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1007\/s11262-007-0194-9","article-title":"Sequence comparison of the right end of fowl adenovirus genomes","volume":"36","author":"Corredor","year":"2008","journal-title":"Virus Genes"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1080\/03079457.2017.1385724","article-title":"Fowl adenovirus-induced diseases and strategies for their control\u2014A review on the current global situation","volume":"47","author":"Schachner","year":"2018","journal-title":"Avian Pathol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1136\/vr.124.10.247","article-title":"Hydropericarditis syndrome in broiler chickens in Pakistan","volume":"124","author":"Anjum","year":"1989","journal-title":"Vet. Rec."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2630","DOI":"10.3382\/ps\/pex087","article-title":"Fowl adenovirus serotype 4: Epidemiology, pathogenesis, diagnostic detection, and vaccine strategies","volume":"96","author":"Li","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e117","DOI":"10.1038\/emi.2016.115","article-title":"Genetic characterization of novel fowl aviadenovirus 4 isolates from outbreaks of hepatitis-hydropericardium syndrome in broiler chickens in China","volume":"5","author":"Liu","year":"2016","journal-title":"Emerg. Microbes Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1038\/s41426-018-0203-1","article-title":"Fiber2 and hexon genes are closely associated with the virulence of the emerging and highly pathogenic fowl adenovirus 4","volume":"7","author":"Zhang","year":"2018","journal-title":"Emerg. Microbes Infect."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1099\/vir.0.19497-0","article-title":"Genetic content and evolution of adenoviruses","volume":"84","author":"Davison","year":"2003","journal-title":"J. Gen. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1099\/vir.0.030064-0","article-title":"Coding potential and transcript analysis of fowl adenovirus 4: Insight into upstream ORFs as common sequence features in adenoviral transcripts","volume":"92","author":"Griffin","year":"2011","journal-title":"J. Gen. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/s11262-005-0031-y","article-title":"Sequence analysis of the left end of fowl adenovirus genomes","volume":"33","author":"Corredor","year":"2006","journal-title":"Virus Genes"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1261\/rna.078937.121","article-title":"Nanopore sequencing of RNA and cDNA molecules in Escherichia coli","volume":"28","author":"Grunberger","year":"2022","journal-title":"RNA"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3359","DOI":"10.1038\/s41467-019-11272-z","article-title":"A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes","volume":"10","author":"Soneson","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1038\/s41467-019-08734-9","article-title":"Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen","volume":"10","author":"Depledge","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1038\/s41587-021-01108-x","article-title":"Nanopore sequencing technology, bioinformatics and applications","volume":"39","author":"Wang","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jviromet.2018.04.001","article-title":"DNA assembly technique simplifies the construction of infectious clone of fowl adenovirus","volume":"257","author":"Zou","year":"2018","journal-title":"J. Virol. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1038\/s41587-019-0201-4","article-title":"Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype","volume":"37","author":"Kim","year":"2019","journal-title":"Nat. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"giab008","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"Gigascience"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/0471250953.bi1112s47","article-title":"BEDTools: The Swiss-Army Tool for Genome Feature Analysis","volume":"47","author":"Quinlan","year":"2014","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"644","DOI":"10.1038\/nbt.1883","article-title":"Full-length transcriptome assembly from RNA-Seq data without a reference genome","volume":"29","author":"Grabherr","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1093\/bioinformatics\/bty191","article-title":"Minimap2: Pairwise alignment for nucleotide sequences","volume":"34","author":"Li","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3506","DOI":"10.1093\/bioinformatics\/btu538","article-title":"LoRDEC: Accurate and efficient long read error correction","volume":"30","author":"Salmela","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"304","DOI":"10.12688\/f1000research.23297.1","article-title":"GFF Utilities: GffRead and GffCompare","volume":"9","author":"Pertea","year":"2020","journal-title":"F1000Res"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Shumate, A., Wong, B., Pertea, G., and Pertea, M. (2022). Improved transcriptome assembly using a hybrid of long and short reads with StringTie. PLoS Comput. Biol., 18.","DOI":"10.1371\/journal.pcbi.1009730"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1080\/21541264.2015.1067286","article-title":"ElemeNT: A computational tool for detecting core promoter elements","volume":"6","author":"Sloutskin","year":"2015","journal-title":"Transcription"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Zheng, Y., Wang, H., Zhang, Y., Gao, X., Xing, E.P., and Xu, M. (2020). Poly(A)-DG: A deep-learning-based domain generalization method to identify cross-species Poly(A) signal without prior knowledge from target species. PLoS Comput. Biol., 16.","DOI":"10.1371\/journal.pcbi.1008297"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Shulman, E.D., and Elkon, R. (2020). Systematic identification of functional SNPs interrupting 3\u2032UTR polyadenylation signals. PLoS Genet., 16.","DOI":"10.1371\/journal.pgen.1008977"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1093\/nar\/gki158","article-title":"A large-scale analysis of mRNA polyadenylation of human and mouse genes","volume":"33","author":"Tian","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Shen, W., Le, S., Li, Y., and Hu, F. (2016). SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA\/Q File Manipulation. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0163962"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3019","DOI":"10.1093\/bioinformatics\/btab090","article-title":"orfipy: A fast and flexible tool for extracting ORFs","volume":"37","author":"Singh","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_42","first-page":"656","article-title":"BLAT--the BLAST-like alignment tool","volume":"12","author":"Kent","year":"2002","journal-title":"Genome Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1093\/bib\/bbr078","article-title":"Visualizing next-generation sequencing data with JBrowse","volume":"14","author":"Westesson","year":"2013","journal-title":"Brief Bioinform."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1630","DOI":"10.1101\/gr.094607.109","article-title":"JBrowse: A next-generation genome browser","volume":"19","author":"Skinner","year":"2009","journal-title":"Genome Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/nbt.1754","article-title":"Integrative genomics viewer","volume":"29","author":"Robinson","year":"2011","journal-title":"Nat. Biotechnol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1007\/s007050050309","article-title":"The major late promoter and bipartite leader sequence of fowl adenovirus","volume":"143","author":"Sheppard","year":"1998","journal-title":"Arch. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1128\/jvi.64.2.551-558.1990","article-title":"The adenovirus type 5 i-leader open reading frame functions in cis to reduce the half-life of L1 mRNAs","volume":"64","author":"Soloway","year":"1990","journal-title":"J. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e0047022","DOI":"10.1128\/spectrum.00470-22","article-title":"No Genus-Specific Gene Is Essential for the Replication of Fowl Adenovirus 4 in Chicken LMH Cells","volume":"10","author":"Liu","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6517","DOI":"10.1128\/JVI.73.8.6517-6525.1999","article-title":"Characterization of CELO virus proteins that modulate the pRb\/E2F pathway","volume":"73","author":"Lehrmann","year":"1999","journal-title":"J. Virol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.1128\/jvi.71.4.3168-3177.1997","article-title":"Identification of a novel antiapoptotic protein, GAM-1, encoded by the CELO adenovirus","volume":"71","author":"Chiocca","year":"1997","journal-title":"J. Virol."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Adamopoulos, P.G., Tsiakanikas, P., Stolidi, I., and Scorilas, A. (2022). A versatile 5\u2032 RACE-Seq methodology for the accurate identification of the 5\u2032 termini of mRNAs. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08386-y"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-981-13-6037-4_1","article-title":"Strategies for Converting RNA to Amplifiable cDNA for Single-Cell RNA Sequencing Methods","volume":"1129","author":"Sasagawa","year":"2019","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"892","DOI":"10.2144\/01304pf02","article-title":"Reverse transcriptase template switching: A SMART approach for full-length cDNA library construction","volume":"30","author":"Zhu","year":"2001","journal-title":"Biotechniques"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1038\/s41592-019-0617-2","article-title":"Nanopore native RNA sequencing of a human poly(A) transcriptome","volume":"16","author":"Workman","year":"2019","journal-title":"Nat. Methods"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Chen, Y., Davidson, N.M., Wan, Y.K., Patel, H., Yao, F., Low, H.M., Hendra, C., Watten, L., Sim, A., and Sawyer, C. (2021). A systematic benchmark of Nanopore long read RNA sequencing for transcript level analysis in human cell lines. bioRxiv.","DOI":"10.1101\/2021.04.21.440736"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1038\/s41580-018-0028-8","article-title":"Eukaryotic core promoters and the functional basis of transcription initiation","volume":"19","author":"Haberle","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1146\/annurev.ge.20.120186.000401","article-title":"Adenovirus promoters and E1A transactivation","volume":"20","author":"Berk","year":"1986","journal-title":"Annu. Rev. Genet."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/0092-8674(79)90333-7","article-title":"Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs","volume":"17","author":"Berk","year":"1979","journal-title":"Cell"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1128\/JVI.78.4.1782-1791.2004","article-title":"Activation of the early-late switch in adenovirus type 5 major late transcription unit expression by L4 gene products","volume":"78","author":"Farley","year":"2004","journal-title":"J. Virol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"4450","DOI":"10.1128\/jvi.68.7.4450-4457.1994","article-title":"The product of the adenovirus intermediate gene IVa2 is a transcriptional activator of the major late promoter","volume":"68","author":"Tribouley","year":"1994","journal-title":"J. Virol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/2\/529\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:34:52Z","timestamp":1760121292000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/2\/529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,14]]},"references-count":60,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["v15020529"],"URL":"https:\/\/doi.org\/10.3390\/v15020529","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,14]]}}}