{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T21:18:44Z","timestamp":1790889524433,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Joy Liebenberg Trust, the South African Government Treasury\u2019s Economic Competitive Support Programme and Gauteng Department of Agriculture and Rural Development (GDARD)"},{"name":"Prof. M.-L.P. of the University of Pretoria, Faculty of Veterinary Science, South Africa"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Rift valley fever (RVF), caused by the RVF virus (RVFV), is a vector-borne zoonotic disease that primarily affects domestic ruminants. Abortion storms and neonatal deaths characterise the disease in animals. Humans develop flu-like symptoms, which can progress to severe disease. The susceptibility of domestic pigs (Sus scrofa domesticus) to RVFV remains unresolved due to conflicting experimental infection results. To address this, we infected two groups of pregnant sows, neonates and weaners, each with a different RVFV isolate, and a third group of weaners with a mixture of the two viruses. Serum, blood and oral, nasal and rectal swabs were collected periodically, and two neonates and a weaner from group 1 and 2 euthanised from 2 days post infection (DPI), with necropsy and histopathology specimens collected. Sera and organ pools, blood and oronasorectal swabs were tested for RVFV antibodies and RNA. Results confirmed that pigs can be experimentally infected with RVFV, although subclinically, and that pregnant sows can abort following infection. Presence of viral RNA in oronasorectal swab pools on 28 DPI suggest that pigs may shed RVFV for at least one month. It is concluded that precautions should be applied when handling pig body fluids and carcasses during RVF outbreaks.<\/jats:p>","DOI":"10.3390\/v15020545","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T02:28:27Z","timestamp":1676514507000},"page":"545","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Experimental Infection of Domestic Pigs (Sus scrofa) with Rift Valley Fever Virus"],"prefix":"10.3390","volume":"15","author":[{"given":"Baratang Alison","family":"Lubisi","sequence":"first","affiliation":[{"name":"Agricultural Research Council\u2014Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa"},{"name":"Department of Zoology and Entomology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Hatfield, Pretoria 0028, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4057-0908","authenticated-orcid":false,"given":"Paidamwoyo Barry","family":"Mutowembwa","sequence":"additional","affiliation":[{"name":"Agricultural Research Council\u2014Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phumudzo Nomicia","family":"Ndouvhada","sequence":"additional","affiliation":[{"name":"Agricultural Research Council\u2014Onderstepoort Veterinary Research, Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3949-7283","authenticated-orcid":false,"given":"Lieza","family":"Odendaal","sequence":"additional","affiliation":[{"name":"Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9223-4204","authenticated-orcid":false,"given":"Armanda D. S.","family":"Bastos","sequence":"additional","affiliation":[{"name":"Department of Zoology and Entomology, Faculty of Natural and Agricultural Sciences, University of Pretoria, Hatfield, Pretoria 0028, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6924-4060","authenticated-orcid":false,"given":"Mary-Louise","family":"Penrith","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1002\/path.1700340418","article-title":"Enzootic hepatitis or Rift Valley fever. An undescribed virus disease of sheep cattle and man from east Africa","volume":"34","author":"Daubney","year":"1931","journal-title":"J. Pathol. Bacteriol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2295","DOI":"10.1007\/s00705-018-3843-5","article-title":"Taxonomy of the family Arenaviridae and the order Bunyavirales: Update 2018","volume":"163","author":"Maes","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"2165","DOI":"10.3201\/eid2212.160310","article-title":"Rift valley fever Outbreak in Livestock, Mozambique, 2014","volume":"22","author":"Fafetine","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_4","first-page":"1020","article-title":"A Review on Rift valley fever on Animal, Human Health and its Impact on Live Stock Marketing","volume":"3","author":"Kasye","year":"2016","journal-title":"Austin Virol. Retrovirol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1177\/0300985819882633","article-title":"Lesions and Cellular Tropism of Natural Rift valley fever Virus Infection in Young Lambs","volume":"57","author":"Odendaal","year":"2020","journal-title":"Vet. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Odendaal, L., Davis, A.S., and Venter, E.H. (2021). Insights into the Pathogenesis of Viral Haemorrhagic Fever Based on Virus Tropism and Tissue Lesions of Natural Rift valley fever. Viruses, 13.","DOI":"10.3390\/v13040709"},{"key":"ref_7","unstructured":"OIE (2021, August 23). Rift Valley Fever (Infection with Rift Valley Fever Virus). Available online: https:\/\/www.oie.int\/fileadmin\/Home\/eng\/Animal_Health_in_the_World\/docs\/pdf\/Disease_cards\/RIFT_VALLEY_FEVER.pdf."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.coviro.2012.02.017","article-title":"Breaking the chain: Rift Valley fever virus control via livestock vaccination","volume":"2","author":"Bird","year":"2012","journal-title":"Curr. Opin. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"61","DOI":"10.2987\/19-6883.1","article-title":"Texas mosquito control response following Hurricane Harvey","volume":"36","author":"Qualls","year":"2020","journal-title":"J. Am. Mosq. Control. Assoc."},{"key":"ref_10","unstructured":"WHO (2021, August 23). Rift Valley Fever Outbreaks Forecasting Models. Available online: https:\/\/www.who.int\/publications\/i\/item\/rift-valley-fever-outbreaks-forecasting-models."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1128\/CMR.18.4.608-637.2005","article-title":"Biological transmission of arboviruses: Reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends","volume":"18","author":"Kuno","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/j.prevetmed.2004.02.007","article-title":"Characterisation of smallholder pig production in Kikuyu Division, central Kenya","volume":"63","author":"Wabacha","year":"2004","journal-title":"Prev. Vet. Med."},{"key":"ref_13","first-page":"331","article-title":"The potential role of pigs in the enzootic cycle of rift valley Fever at Alexandria Governorate, Egypt","volume":"84","author":"Youssef","year":"2009","journal-title":"J. Egypt. Public Health Assoc."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Thomas, L.F., de Glanville, W.A., Cook, E.A., and F\u00e8vre, E.M. (2013). The spatial ecology of free-ranging domestic pigs (Sus scrofa) in western Kenya. BMC Veter- Res., 9.","DOI":"10.1186\/1746-6148-9-46"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1038\/200919a0","article-title":"Pigs and Rift valley fever","volume":"200","author":"Scott","year":"1963","journal-title":"Nature"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"182","DOI":"10.4269\/ajtmh.21-0188","article-title":"Experimental Infection of Domestic Piglets (Sus scrofa) with Rift valley fever Virus","volume":"106","author":"Clarke","year":"2021","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"241","DOI":"10.7589\/0090-3558-48.2.241","article-title":"The role of wild mammals in the maintenance of Rift Valley fever virus","volume":"48","author":"Olive","year":"2012","journal-title":"J. Wildl. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e1179","DOI":"10.1111\/tbed.14466","article-title":"One hundred years of African swine fever in Africa: Where have we been, where are we now, where are we going?","volume":"69","author":"Penrith","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_19","first-page":"470","article-title":"The pathogenesis of Rift valley fever in lambs","volume":"23","author":"Easterday","year":"1962","journal-title":"Am. J. Vet. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.1016\/S0140-6736(00)99624-7","article-title":"The virus of rift valley fever or enzootic hepatitis","volume":"221","author":"Findlay","year":"1931","journal-title":"Lancet"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Maluleke, M.R., Phosiwa, M., van Schalkwyk, A., Michuki, G., Lubisi, B.A., Kegakilwe, P.S., Kemp, S.J., and Majiwa, P.A.O. (2019). A comparative genome analysis of Rift valley fever virus isolates from foci of the disease outbreak in South Africa in 2008\u20132010. PLoS Negl. Trop. Dis., 13.","DOI":"10.1371\/journal.pntd.0006576"},{"key":"ref_22","unstructured":"Robertshaw, D. (2004). Dukes\u2019 Physiology of Domestic Animals, Cornell University. [12th ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2323","DOI":"10.1128\/JCM.40.7.2323-2330.2002","article-title":"Rapid detection and quantification of RNA of Ebola and Marburg viruses, Lassa virus, Crimean-Congo hemorrhagic fever virus, Rift Valley fever virus, dengue virus, and yellow fever virus by real-time reverse transcription-PCR","volume":"40","author":"Drosten","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"E1-5","DOI":"10.4102\/ojvr.v81i2.717","article-title":"Epidemiological study of Rift Valley fever virus in Kigoma, Tanzania","volume":"81","author":"Kifaro","year":"2014","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_25","unstructured":"(2022, August 25). Standard Nucleotide BLAST, Available online: https:\/\/blast.ncbi.nlm.nih.gov\/Blast.cgi."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_27","first-page":"406","article-title":"The neighbor-joining method: A new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence limits on phylogenies: An approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1177\/1040638713516759","article-title":"Sensitivity and specificity of real-time reverse transcription polymerase chain reaction, histopathology, and immunohistochemical labeling for the detection of Rift Valley fever virus in naturally infected cattle and sheep","volume":"26","author":"Odendaal","year":"2014","journal-title":"J. Veter. Diagn. Investig."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1251\/bpo70","article-title":"Negative Staining and Image Classification\u2014Powerful Tools in Modern Electron Microscopy","volume":"6","author":"Ohi","year":"2004","journal-title":"Biol. Proced. Online"},{"key":"ref_31","unstructured":"(2022, March 16). GraphPad Prism\u2014QuickCalcs. Available online: https:\/\/www.graphpad.com\/quickcalcs\/."},{"key":"ref_32","unstructured":"(2022, March 16). Statistics Kingdom. Available online: https:\/\/www.statskingdom.com\/170median_mann_whitney.html."},{"key":"ref_33","unstructured":"(2022, March 16). MedCalc Easy-To-Use Statistical Software. Free Statistical Calculators: Diagnostic Test Evaluation Calculator. Available online: https:\/\/www.medcalc.org\/calc\/diagnostic_test.php."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1177\/0300985820954549","article-title":"Ovine Fetal and Placental Lesions and Cellular Tropism in Natural Rift valley fever Virus Infections","volume":"57","author":"Odendaal","year":"2020","journal-title":"Vet. Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.virol.2015.12.003","article-title":"Development of a sheep challenge model for Rift Valley fever","volume":"489","author":"Faburay","year":"2016","journal-title":"Virology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"R1525","DOI":"10.1152\/ajpregu.00751.2004","article-title":"Induction of overlapping genes by fasting and a peroxisome proliferator in pigs: Evidence of functional PPARalpha in nonproliferating species","volume":"288","author":"Cheon","year":"2005","journal-title":"Am. J. Physiol. Integr. Comp. Physiol."},{"key":"ref_37","first-page":"24","article-title":"Reproductive diseases in sows (Sus scrofa domestica): A Review","volume":"67","author":"Pozzi","year":"2012","journal-title":"J. Vet. Med."},{"key":"ref_38","first-page":"431","article-title":"Rift valley fever: A review","volume":"5","author":"Weiss","year":"1957","journal-title":"Bull. Epizoot. Dis. Afr."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1089\/vbz.2012.1160","article-title":"Vertical transmission of Rift Valley fever virus without detectable maternal viremia","volume":"13","author":"Antonis","year":"2013","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"28","DOI":"10.54846\/jshap\/578","article-title":"Anatomical abnormalities in a group of finishing pigs: Prevalence and pig performance","volume":"17","author":"Straw","year":"2009","journal-title":"J. Swine Health Prod."},{"key":"ref_41","first-page":"153","article-title":"Brain teratology as a result of transplacental virus infection in ruminants","volume":"51","author":"Coetzer","year":"1980","journal-title":"J. South Afr. Veter- Assoc."},{"key":"ref_42","first-page":"119","article-title":"Hydrops amnii in sheep associated with hydranencephaly and arthrogryposis with wesselsbron disease and rift valley fever viruses as aetiological agents","volume":"44","author":"Coetzer","year":"1977","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/s41541-019-0138-0","article-title":"Safety and efficacy of ChAdOx1 RVF vaccine against Rift Valley fever in pregnant sheep and goats","volume":"4","author":"Stedman","year":"2019","journal-title":"NPJ Vaccines"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1001\/jama.1997.03540450065037","article-title":"Determination of deltoid fat pad thickness. Implications for needle length in adult immunization","volume":"277","author":"Poland","year":"1997","journal-title":"J. Am. Med. Assoc."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.virusres.2012.10.012","article-title":"Antibody-mediated neutralization of African swine fever virus: Myths and facts","volume":"173","author":"Escribano","year":"2013","journal-title":"Virus Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1645\/0022-3395(2001)087[0108:TTOTGI]2.0.CO;2","article-title":"Transplacental transmission of Toxoplasma Gondii in minipigs infected with strains of different virulence","volume":"7","author":"Jungersen","year":"2001","journal-title":"J. Parasitol."},{"key":"ref_47","unstructured":"Comtet, L., Mari\u00e9, J.-L., Davoust, B., Cetre-Sossah, C., and Pourquier, P. (2010, January 15\u201317). Preliminary validation of the ID Screen\u00ae Rift valley fever Competition Multi-species ELISA [Poster presented]. Proceedings of the EAVLD Conference, Lelystad, The Netherlands. Available online: https:\/\/www.id-vet.com\/pdfs\/newsletter1\/poster_riftc.pdf."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Lubisi, B.A., Ndouvhada, P.N., Neiffer, D., Penrith, M.-L., Sibanda, D.R., and Bastos, A. (2019). Evaluation of a Virus Neutralisation Test for Detection of Rift valley fever Antibodies in Suid Sera. Trop. Med. Infect. Dis., 4.","DOI":"10.3390\/tropicalmed4010052"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1007\/978-1-60327-353-4_2","article-title":"Overcoming Inhibition in Real-Time Diagnostic PCR","volume":"943","author":"Hedman","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"ref_50","first-page":"31","article-title":"Pre-PCR Processing of samples","volume":"216","author":"Radstrom","year":"2003","journal-title":"Methods Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"907095","DOI":"10.1100\/2012\/907095","article-title":"An Optimized Real-Time PCR to Avoid Species-\/Tissue-Associated Inhibition for H5N1 Detection in Ferret and Monkey Tissues","volume":"2012","author":"Zhan","year":"2012","journal-title":"Sci. World J."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.mam.2005.12.003","article-title":"RNA integrity and the effect on the real-time qRT-PCR performance","volume":"27","author":"Fleige","year":"2006","journal-title":"Mol. Aspects Med."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/s11259-017-9686-9","article-title":"Sample pooling for real-time PCR detection and virulence determination of the footrot pathogen Dichelobacter nodosus","volume":"41","author":"Frosth","year":"2017","journal-title":"Vet. Res. Commun."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Muniesa, A., Ferreira, C., Fuertes, H., Halaihel, N., and de Blas, I. (2014). Estimation of the Relative Sensitivity of qPCR Analysis Using Pooled Samples. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0093491"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.2460\/ajvr.1994.55.10.1391","article-title":"Temporal characterization of transplacental infection of porcine fetuses with porcine reproductive and respiratory syndrome virus","volume":"55","author":"Mengeling","year":"1994","journal-title":"Am. J. Vet. Res."},{"key":"ref_56","first-page":"287","article-title":"Co-housing of Rift valley fever Virus Infected Lambs with Immunocompetent or Immunosuppressed Lambs Does Not Result in Virus Transmission","volume":"7","author":"Kant","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_57","first-page":"1559","article-title":"Evaluation of a formalin-inactivated Rift Valley fever vaccine in sheep","volume":"41","author":"Harrington","year":"1980","journal-title":"Am. J. Vet. Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1089\/vbz.2009.0205","article-title":"Experimental infection of young adult European breed sheep with Rift Valley fever virus field isolates","volume":"10","author":"Busquets","year":"2010","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2270","DOI":"10.3201\/eid1712.111035","article-title":"Molecular Epidemiology of Rift valley fever Virus","volume":"17","author":"Grobbelaar","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.virol.2015.03.022","article-title":"Viral quasispecies","volume":"479\u2013480","author":"Andino","year":"2015","journal-title":"Virology"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/0092-8674(78)90223-4","article-title":"Nucleotide sequence heterogeneity of an RNA phage population","volume":"13","author":"Domingo","year":"1978","journal-title":"Cell"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Dudas, G., and Bedford, T. (2019). The ability of single genes vs full genomes to resolve time and space in outbreak analysis. BMC Evol. Biol., 19.","DOI":"10.1186\/s12862-019-1567-0"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11262-018-1611-y","article-title":"Molecular aspects of Rift Valley fever virus and the emergence of reassortants","volume":"55","author":"Gaudreault","year":"2019","journal-title":"Virus Genes"},{"key":"ref_64","first-page":"205","article-title":"The pathology of Rift Valley fever. I. Lesions occurring in natural cases in new-born lambs","volume":"44","author":"Coetzer","year":"1977","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_65","first-page":"11","article-title":"The pathology of Rift Valley fever. II. Lesions occurring in field cases in adult cattle, calves and aborted foetuses","volume":"49","author":"Coetzer","year":"1982","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1177\/0300985818806049","article-title":"Lesions and Cellular Tropism of Natural Rift valley fever Virus Infection in Adult Sheep","volume":"56","author":"Odendaal","year":"2019","journal-title":"Vet. Pathol."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Damjanov, I. (2009). Pathology Secrets, ScienceDirect. [3rd ed.].","DOI":"10.1016\/B978-0-323-05594-9.00001-5"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/BF00202202","article-title":"The nature and significance of liver cell vacuolation following hepatocellular injury\u2014An analysis based on observations on rats rendered tolerant to hepatotoxic damage","volume":"428","author":"Nayak","year":"1996","journal-title":"Virchows Arch."},{"key":"ref_69","unstructured":"(2022, June 10). Maluleke and Rift Valley Fever, Available online: https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/?term=Maluleke+and+rift+valley+fever."},{"key":"ref_70","unstructured":"(2022, June 10). Grobbelaar and Rift Valley Fever, Available online: https:\/\/www.ncbi.nlm.nih.gov\/nuccore\/?term=Grobbelaar+and+rift+valley+fever."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/2\/545\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:37:21Z","timestamp":1760121441000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/2\/545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,16]]},"references-count":70,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["v15020545"],"URL":"https:\/\/doi.org\/10.3390\/v15020545","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,16]]}}}