{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:54:29Z","timestamp":1792806869802,"version":"4.5.0"},"reference-count":49,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T00:00:00Z","timestamp":1679529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","doi-asserted-by":"publisher","award":["398066876\/GRK 2485\/1"],"award-info":[{"award-number":["398066876\/GRK 2485\/1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","doi-asserted-by":"publisher","award":["491094227"],"award-info":[{"award-number":["491094227"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Alexander von Humboldt Foundation","award":["398066876\/GRK 2485\/1"],"award-info":[{"award-number":["398066876\/GRK 2485\/1"]}],"id":[{"id":"https:\/\/ror.org\/012kf4317","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Alexander von Humboldt Foundation","award":["491094227"],"award-info":[{"award-number":["491094227"]}],"id":[{"id":"https:\/\/ror.org\/012kf4317","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University of Veterinary Medicine Hannover, Foundation","award":["398066876\/GRK 2485\/1"],"award-info":[{"award-number":["398066876\/GRK 2485\/1"]}],"id":[{"id":"https:\/\/ror.org\/015qjqf64","id-type":"ROR","asserted-by":"crossref"}]},{"name":"University of Veterinary Medicine Hannover, Foundation","award":["491094227"],"award-info":[{"award-number":["491094227"]}],"id":[{"id":"https:\/\/ror.org\/015qjqf64","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Dengue virus serotypes 1 to 4 (DENV1\u20134) place nearly half the global population at risk of infection and the licenced tetravalent dengue vaccine fails to protect individuals who have not previously been exposed to DENV. The development of intervention strategies had long been hampered by the lack of a suitable small animal model. DENV does not replicate in wild-type mice due to its inability to antagonise the mouse type I interferon (IFN) response. Mice deficient in type I IFN signalling (Ifnar1\u2212\/\u2212 mice) are highly susceptible to DENV infection, but their immunocompromised status makes it difficult to interpret immune responses elicited by experimental vaccines. To develop an alternative mouse model for vaccine testing, we treated adult wild-type mice with MAR1-5A3\u2014an IFNAR1-blocking, non-cell-depleting antibody\u2014prior to infection with the DENV2 strain D2Y98P. This approach would allow for vaccination of immunocompetent mice and subsequent inhibition of type I IFN signalling prior to challenge infection. While Ifnar1\u2212\/\u2212 mice quickly succumbed to infection, MAR1-5A3-treated mice did not show any signs of illness but eventually seroconverted. Infectious virus was recovered from the sera and visceral organs of Ifnar1\u2212\/\u2212 mice, but not from those of mice treated with MAR1-5A3. However, high levels of viral RNA were detected in the samples of MAR1-5A3-treated mice, indicating productive viral replication and dissemination. This transiently immunocompromised mouse model of DENV2 infection will aid the pre-clinical assessment of next-generation vaccines as well as novel antiviral treatments.<\/jats:p>","DOI":"10.3390\/v15040814","type":"journal-article","created":{"date-parts":[[2023,3,23]],"date-time":"2023-03-23T03:07:57Z","timestamp":1679540877000},"page":"814","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Transient Blockade of Type I Interferon Signalling Promotes Replication of Dengue Virus Strain D2Y98P in Adult Wild-Type Mice"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5678-3089","authenticated-orcid":false,"given":"Lucas","family":"Wilken","sequence":"first","affiliation":[{"name":"Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine (TiHo), 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8804-9092","authenticated-orcid":false,"given":"Sonja","family":"Stelz","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine (TiHo), 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6631-2096","authenticated-orcid":false,"given":"Chittappen Kandiyil","family":"Prajeeth","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine (TiHo), 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1921-4581","authenticated-orcid":false,"given":"Guus F.","family":"Rimmelzwaan","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine (TiHo), 30559 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1128\/CMR.00035-09","article-title":"Dengue virus pathogenesis: An integrated view","volume":"22","author":"Martina","year":"2009","journal-title":"Clin. 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