{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T23:47:29Z","timestamp":1788306449250,"version":"build-2803163510"},"reference-count":51,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"German Center of Infection Research","doi-asserted-by":"publisher","award":["8033701711"],"award-info":[{"award-number":["8033701711"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009139","name":"German Center of Infection Research","doi-asserted-by":"publisher","award":["284237345"],"award-info":[{"award-number":["284237345"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["8033701711"],"award-info":[{"award-number":["8033701711"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["284237345"],"award-info":[{"award-number":["284237345"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pandemie Netzwerk Hessen","award":["8033701711"],"award-info":[{"award-number":["8033701711"]}]},{"name":"Pandemie Netzwerk Hessen","award":["284237345"],"award-info":[{"award-number":["284237345"]}]},{"name":"Coropan","award":["8033701711"],"award-info":[{"award-number":["8033701711"]}]},{"name":"Coropan","award":["284237345"],"award-info":[{"award-number":["284237345"]}]},{"name":"Open Acess Publishing Fund of Philipps-Universit\u00e4t Marburg","award":["8033701711"],"award-info":[{"award-number":["8033701711"]}]},{"name":"Open Acess Publishing Fund of Philipps-Universit\u00e4t Marburg","award":["284237345"],"award-info":[{"award-number":["284237345"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["8033701711"],"award-info":[{"award-number":["8033701711"]}]},{"name":"Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)","award":["284237345"],"award-info":[{"award-number":["284237345"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes cellular trafficking pathways to process its structural proteins and move them to the site of assembly. Nevertheless, the exact process of assembly and subcellular trafficking of SARS-CoV-2 proteins remains largely unknown. Here, we have identified and characterized Rab1B as an important host factor for the trafficking and maturation of the spike protein (S) after synthesis at the endoplasmic reticulum (ER). Using confocal microscopy, we showed that S and Rab1B substantially colocalized in compartments of the early secretory pathway. Co-expression of dominant-negative (DN) Rab1B N121I leads to an aberrant distribution of S into perinuclear spots after ectopic expression and in SARS-CoV-2-infected cells caused by either structural rearrangement of the ERGIC or Golgi or missing interaction between Rab1B and S. Western blot analyses revealed a complete loss of the mature, cleaved S2 subunit in cell lysates and culture supernatants upon co-expression of DN Rab1B N121I. In sum, our studies indicate that Rab1B is an important regulator of trafficking and maturation of SARS-CoV-2 S, which not only improves our understanding of the coronavirus replication cycle but also may have implications for the development of antiviral strategies.<\/jats:p>","DOI":"10.3390\/v15040824","type":"journal-article","created":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T04:31:05Z","timestamp":1679632265000},"page":"824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Inhibition of Rab1B Impairs Trafficking and Maturation of SARS-CoV-2 Spike Protein"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3221-8447","authenticated-orcid":false,"given":"Christopher","family":"Veeck","sequence":"first","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6061-8019","authenticated-orcid":false,"given":"Nadine","family":"Biedenkopf","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, 35043 Marburg, Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4752-8326","authenticated-orcid":false,"given":"Cornelius","family":"Rohde","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, 35043 Marburg, Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6621-9350","authenticated-orcid":false,"given":"Stephan","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, 35043 Marburg, Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro","family":"Halwe","sequence":"additional","affiliation":[{"name":"Institute of Virology, Philipps University Marburg, 35043 Marburg, Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Giessen-Marburg-Langen, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2021, June 14). 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