{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:16:20Z","timestamp":1792790180215,"version":"4.5.0"},"reference-count":53,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U19A2038"],"award-info":[{"award-number":["U19A2038"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2016YFD0501300"],"award-info":[{"award-number":["2016YFD0501300"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Key R&amp;D Plan","award":["U19A2038"],"award-info":[{"award-number":["U19A2038"]}]},{"name":"National Key R&amp;D Plan","award":["2016YFD0501300"],"award-info":[{"award-number":["2016YFD0501300"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Salmonella pullorum causes typical \u201cBacillary White Diarrhea\u201d and loss of appetite in chicks, which leads to the death of chicks in severe cases; thus, it is still a critical issue in China. Antibiotics are conventional medicines used for Salmonella infections; however, due to the extensive long-term use and even abuse of antibiotics, drug resistance becomes increasingly severe, making treating pullorum disease more difficult. Most of the endolysins are hydrolytic enzymes produced by bacteriophages to cleave the host\u2019s cell wall during the final stage of the lytic cycle. A virulent bacteriophage, YSP2, of Salmonella was isolated in a previous study. A Pichia pastoris expression strain that can express the Salmonella bacteriophage endolysin was constructed efficiently, and the Gram-negative bacteriophage endolysin, LySP2, was obtained in this study. Compared with the parental phage YSP2, which can only lyse Salmonella, LySP2 can lyse Salmonella and Escherichia. The survival rate of Salmonella-infected chicks treated with LySP2 can reach up to 70% and reduce Salmonella abundance in the liver and intestine. The treatment group showed that LySP2 significantly improved the health of infected chicks and alleviated organ damage caused by Salmonella infection. In this study, the Salmonella bacteriophage endolysin was expressed efficiently by Pichia pastoris, and the endolysin LySP2 showed good potential for the treatment of pullorum disease caused by Salmonella pullorum.<\/jats:p>","DOI":"10.3390\/v15040836","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T04:31:21Z","timestamp":1679891481000},"page":"836","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The Broad-Spectrum Endolysin LySP2 Improves Chick Survival after Salmonella Pullorum Infection"],"prefix":"10.3390","volume":"15","author":[{"given":"Hewen","family":"Deng","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjiao","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chencheng","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanyun","family":"Song","sequence":"additional","affiliation":[{"name":"Changchun Customs District, Changchun 130000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0010-5008","authenticated-orcid":false,"given":"Xin","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Jilin University, Xi\u2019an Street 5333#, Changchun 130062, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/03079457.2010.542575","article-title":"Pullorum disease and fowl typhoid\u2014New thoughts on old diseases: A review","volume":"40","author":"Barrow","year":"2011","journal-title":"Avian Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"405","DOI":"10.20506\/rst.19.2.1222","article-title":"Fowl typhoid and pullorum disease","volume":"19","author":"Shivaprasad","year":"2000","journal-title":"Rev. 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