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However, there are few efficacious nAbs against classical swine fever virus (CSFV) that have been produced, especially the porcine-originated nAbs. In this study, we generated three porcine monoclonal antibodies (mAbs) with in vitro neutralizing activity against CSFV, aiming to facilitate the development of passive antibody vaccines or antiviral drugs against CSFV that offer the advantages of stability and low immunogenicity. Pigs were immunized with the C-strain E2 (CE2) subunit vaccine, KNB-E2. At 42 days post vaccination (DPV), CE2-specific single B cells were isolated via fluorescent-activated cell sorting (FACS) baited by Alexa Fluor\u2122 647-labeled CE2 (positive), goat anti-porcine IgG (H + L)-FITC antibody (positive), PE mouse anti-pig CD3\u03b5 (negative) and PE mouse anti-pig CD8a (negative). The full coding region of IgG heavy (H) chains and light (L) chains was amplified by reverse transcription-polymerase chain reaction (RT-PCR). Overall, we obtained 3 IgG H chains, 9 kappa L chains and 36 lambda L chains, which include three paired chains (two H + \u03ba and one H + \u03bb). CE2-specific mAbs were successfully expressed in 293T cells with the three paired chains. The mAbs exhibit potent neutralizing activity against CSFVs. They can protect ST cells from infections in vitro with potent IC50 values from 14.43 \u00b5g\/mL to 25.98 \u00b5g\/mL for the CSFV C-strain, and 27.66 \u00b5g\/mL to 42.61 \u00b5g\/mL for the CSFV Alfort strain. This study is the first report to describe the amplification of whole-porcine IgG genes from single B cells of KNB-E2-vaccinated pig. The method is versatile, sensitive, and reliable. The generated natural porcine nAbs can be used to develop long-acting and low-immunogenicity passive antibody vaccine or anti-CSFV agents for CSF control and prevention.<\/jats:p>","DOI":"10.3390\/v15040863","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T06:15:15Z","timestamp":1679984115000},"page":"863","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of Porcine Monoclonal Antibodies with In Vitro Neutralizing Activity against Classical Swine Fever Virus from C-Strain E2-Specific Single B Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7951-4175","authenticated-orcid":false,"given":"Lihua","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Madera","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7894-0233","authenticated-orcid":false,"given":"Douglas P.","family":"Gladue","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, NY 11944, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel V.","family":"Borca","sequence":"additional","affiliation":[{"name":"Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, NY 11944, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5798-3050","authenticated-orcid":false,"given":"Michael T.","family":"McIntosh","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, University of Florida, Gainesville, FL 32611, USA"},{"name":"Child Health Research Institute, Department of Pediatrics, University of Florida, Gainesville, FL 32611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7177-9280","authenticated-orcid":false,"given":"Jishu","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0378-1135(00)00137-1","article-title":"Introduction to classical swine fever: Virus, disease and control policy","volume":"73","author":"Moennig","year":"2000","journal-title":"Vet. 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Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0378-1135(00)00146-2","article-title":"Development of a classical swine fever subunit marker vaccine and companion diagnostic test","volume":"73","author":"Moormann","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Coronado, L., Perera, C.L., Rios, L., Fr\u00edas, M.T., and P\u00e9rez, L.J. (2021). A Critical Review about Different Vaccines against Classical Swine Fever Virus and Their Repercussions in Endemic Regions. Vaccines, 9.","DOI":"10.3390\/vaccines9020154"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/pbi.12986","article-title":"Plant-made E2 glycoprotein single-dose vaccine protects pigs against classical swine fever","volume":"7","author":"Laughlin","year":"2019","journal-title":"Plant Biotechnol. J."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.vetmic.2012.10.030","article-title":"Efficacy of chimeric Pestivirus vaccine candidates against classical swine fever: Protection and DIVA characteristics","volume":"162","author":"Geurts","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1016\/j.vaccine.2012.02.065","article-title":"Towards licensing of CP7_E2alf as marker vaccine against classical swine fever-Duration of immunity","volume":"30","author":"Gabriel","year":"2012","journal-title":"Vaccine"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1177\/1040638715592446","article-title":"Evaluation of an Erns-based enzyme-linked immunosorbent assay to distinguish Classical swine fever virus-infected pigs from pigs vaccinated with CP7_E2alf","volume":"27","author":"Pannhorst","year":"2015","journal-title":"J. Vet. Diagn. Invest."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1038\/s41541-017-0019-3","article-title":"Antibody therapies for the prevention and treatment of viral infections","volume":"2","author":"Salazar","year":"2017","journal-title":"NPJ Vaccines"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nri.2017.148","article-title":"Passive immunotherapy of viral infections: \u2018Super-antibodies\u2019 enter the fray","volume":"18","author":"Walker","year":"2018","journal-title":"Nat. Rev. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12929-019-0592-z","article-title":"Development of therapeutic antibodies for the treatment of diseases","volume":"27","author":"Lu","year":"2020","journal-title":"J. Biomed. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1097\/ACI.0000000000000787","article-title":"Antibody therapy for COVID-19","volume":"21","author":"Marcotte","year":"2021","journal-title":"Curr. Opin. Allergy Clin. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.addr.2020.12.004","article-title":"Challenges and opportunities for antiviral monoclonal antibodies as COVID-19 therapy","volume":"169","author":"Tiruthani","year":"2021","journal-title":"Adv. Drug. Deliv. Rev."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2903","DOI":"10.1016\/j.vaccine.2015.04.065","article-title":"Characterization of a novel oil-in-water emulsion adjuvant for swine influenza virus and Mycoplasma hyopneumoniae vaccines","volume":"33","author":"Pappan","year":"2015","journal-title":"Vaccine"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2137","DOI":"10.4049\/jimmunol.1901483","article-title":"Revisiting the Pig IGHC Gene Locus in Different Breeds Uncovers Nine Distinct IGHG Genes","volume":"205","author":"Zhang","year":"2020","journal-title":"J. Immunol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Wang, L., Mi, S., Madera, R., Ganges, L., Borca, M.V., Ren, J., Cunningham, C., Cino-Ozuna, A.G., Li, H., and Tu, C. (2020). A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post-vaccination monitoring. BMC Vet. Res., 16.","DOI":"10.1186\/s12917-020-2237-6"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1111\/j.1365-2249.1994.tb06145.x","article-title":"Human anti-mouse antibody response to the injection of murine monoclonal antibodies against IL-6","volume":"98","author":"Legouffe","year":"1994","journal-title":"Clin. Exp. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/cr.2007.13","article-title":"Antibody gene therapy: An attractive approach for the treatment of cancers and other chronic diseases","volume":"17","author":"Zheng","year":"2007","journal-title":"Cell Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1038\/nbt0905-1073","article-title":"Monoclonal antibody successes in the clinic","volume":"23","author":"Reichert","year":"2005","journal-title":"Nat. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1016\/j.it.2021.10.008","article-title":"Single B cell technologies for monoclonal antibody discovery","volume":"42","author":"Pedrioli","year":"2021","journal-title":"Trends Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.nbt.2011.03.014","article-title":"Single B cell antibody technologies","volume":"28","author":"Tiller","year":"2011","journal-title":"New Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kurosawa, N., Yoshioka, M., Fujimoto, R., Yamagishi, F., and Isobe, M. (2012). Rapid production of antigen-specific monoclonal antibodies from a variety of animals. BMC Biol., 10.","DOI":"10.1186\/1741-7007-10-80"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Starkie, D.O., Compson, J.E., Rapecki, S., and Lightwood, D.J. (2016). Generation of Recombinant Monoclonal Antibodies from Immunised Mice and Rabbits via Flow Cytometry and Sorting of Antigen-Specific IgG+ Memory B Cells. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0152282"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Ojima-Kato, T., Morishita, S., Uchida, Y., Nagai, S., Kojima, T., and Nakano, H. (2018). Rapid Generation of Monoclonal Antibodies from Single B Cells by Ecobody Technology. Antibodies, 7.","DOI":"10.3390\/antib7040038"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"672","DOI":"10.3389\/fmicb.2019.00672","article-title":"Antigen-Specific Single B Cell Sorting and Monoclonal Antibody Cloning in Guinea Pigs","volume":"10","author":"Lei","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_34","first-page":"1098","article-title":"Extending human IgG half-life using structure-guided design","volume":"10","author":"Booth","year":"2018","journal-title":"mAbs"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1920","DOI":"10.3389\/fimmu.2020.01920","article-title":"Update on Transplacental Transfer of IgG Subclasses: Impact of Maternal and Fetal Factors","volume":"11","author":"Clements","year":"2020","journal-title":"Front. Immunol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/4\/863\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:05:01Z","timestamp":1760123101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/4\/863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,28]]},"references-count":35,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["v15040863"],"URL":"https:\/\/doi.org\/10.3390\/v15040863","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,28]]}}}