{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T11:11:06Z","timestamp":1769598666375,"version":"3.49.0"},"reference-count":83,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["22fk0108627j0001"],"award-info":[{"award-number":["22fk0108627j0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["22K07107"],"award-info":[{"award-number":["22K07107"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["22fk0108627h1101"],"award-info":[{"award-number":["22fk0108627h1101"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["22fk0108627j0001"],"award-info":[{"award-number":["22fk0108627j0001"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["22K07107"],"award-info":[{"award-number":["22K07107"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"JSPS KAKENHI","doi-asserted-by":"publisher","award":["22fk0108627h1101"],"award-info":[{"award-number":["22fk0108627h1101"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["22fk0108627j0001"],"award-info":[{"award-number":["22fk0108627j0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["22K07107"],"award-info":[{"award-number":["22K07107"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"AMED","doi-asserted-by":"publisher","award":["22fk0108627h1101"],"award-info":[{"award-number":["22fk0108627h1101"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Poliovirus (PV) is the causative agent of poliomyelitis and is a target of the global eradication programs of the World Health Organization (WHO). After eradication of type 2 and 3 wild-type PVs, vaccine-derived PV remains a substantial threat against the eradication as well as type 1 wild-type PV. Antivirals could serve as an effective means to suppress the outbreak; however, no anti-PV drugs have been approved at present. Here, we screened for effective anti-PV compounds in a library of edible plant extracts (a total of 6032 extracts). We found anti-PV activity in the extracts of seven different plant species. We isolated chrysophanol and vanicoside B (VCB) as the identities of the anti-PV activities of the extracts of Rheum rhaponticum and Fallopia sachalinensis, respectively. VCB targeted the host PI4KB\/OSBP pathway for its anti-PV activity (EC50 = 9.2 \u03bcM) with an inhibitory effect on in vitro PI4KB activity (IC50 = 5.0 \u03bcM). This work offers new insights into the anti-PV activity in edible plants that may serve as potent antivirals for PV infection.<\/jats:p>","DOI":"10.3390\/v15040903","type":"journal-article","created":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T09:12:59Z","timestamp":1680253979000},"page":"903","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of Anti-Poliovirus Compounds Isolated from Edible Plants"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3314-6626","authenticated-orcid":false,"given":"Minetaro","family":"Arita","sequence":"first","affiliation":[{"name":"Department of Virology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi 208-0011, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6302-4507","authenticated-orcid":false,"given":"Hiroyuki","family":"Fuchino","sequence":"additional","affiliation":[{"name":"Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition, 1-2 Hachimandai, Tsukuba 305-0843, Ibaraki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1038\/291547a0","article-title":"Primary structure, gene organization and polypeptide expression of poliovirus RNA","volume":"291","author":"Kitamura","year":"1981","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1126\/science.aba1238","article-title":"Evolving epidemiology of poliovirus serotype 2 following withdrawal of the serotype 2 oral poliovirus vaccine","volume":"368","author":"Macklin","year":"2020","journal-title":"Science"},{"key":"ref_3","unstructured":"(2023, February 27). WHO. Available online: https:\/\/polioeradication.org\/polio-today\/polio-now\/this-week\/circulating-vaccine-derived-poliovirus\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.15585\/mmwr.mm7133e2","article-title":"Public Health Response to a Case of Paralytic Poliomyelitis in an Unvaccinated Person and Detection of Poliovirus in Wastewater\u2014New York, June-August 2022","volume":"71","author":"Lutterloh","year":"2022","journal-title":"MMWR Morb. Mortal Wkly. Rep."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1531","DOI":"10.1016\/S0140-6736(22)01804-9","article-title":"Sustained detection of type 2 poliovirus in London sewage between February and July, 2022, by enhanced environmental surveillance","volume":"400","author":"Klapsa","year":"2022","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"20586","DOI":"10.2807\/1560-7917.ES2013.18.38.20586","article-title":"Insidious reintroduction of wild poliovirus into Israel, 2013","volume":"18","author":"Anis","year":"2013","journal-title":"Eurosurveillance"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1016\/j.chom.2020.04.003","article-title":"Engineering the Live-Attenuated Polio Vaccine to Prevent Reversion to Virulence","volume":"27","author":"Yeh","year":"2020","journal-title":"Cell Host Microbe"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e67","DOI":"10.1016\/S1473-3099(22)00582-5","article-title":"A novel tool to eradicate an ancient scourge: The novel oral polio vaccine type 2 story","volume":"23","author":"Bandyopadhyay","year":"2023","journal-title":"Lancet Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.antiviral.2008.04.002","article-title":"A case for developing antiviral drugs against polio","volume":"79","author":"Collett","year":"2008","journal-title":"Antiviral Res."},{"key":"ref_10","unstructured":"Committee on Development of a Polio Antiviral and Its Potential Role in Global Poliomyelitis Eradication, National Research Council (2006). Exploring the Role of Antiviral Drugs in the Eradication of Polio: Workshop Report, The National Academies Press."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1002\/med.20125","article-title":"Selective inhibitors of picornavirus replication","volume":"28","author":"Vliegen","year":"2008","journal-title":"Med. Res. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1128\/jvi.66.4.1985-1994.1992","article-title":"Inhibition of poliovirus RNA synthesis by brefeldin A","volume":"66","author":"Maynell","year":"1992","journal-title":"J. Virol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/0042-6822(92)90178-R","article-title":"Involvement of membrane traffic in the replication of poliovirus genomes: Effects of brefeldin A","volume":"191","author":"Irurzun","year":"1992","journal-title":"Virology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1038\/nchembio776","article-title":"Functional characterization of IRESes by an inhibitor of the RNA helicase eIF4A","volume":"2","author":"Bordeleau","year":"2006","journal-title":"Nat. Chem. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1101\/gad.1505307","article-title":"Evolutionary constraints on chaperone-mediated folding provide an antiviral approach refractory to development of drug resistance","volume":"21","author":"Geller","year":"2007","journal-title":"Genes Dev."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5777","DOI":"10.1073\/pnas.1101143108","article-title":"Broad-spectrum antiviral that interferes with de novo pyrimidine biosynthesis","volume":"108","author":"Hoffmann","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115315","DOI":"10.18632\/oncotarget.23258","article-title":"Gemcitabine, a broad-spectrum antiviral drug, suppresses enterovirus infections through innate immunity induced by the inhibition of pyrimidine biosynthesis and nucleotide depletion","volume":"8","author":"Lee","year":"2017","journal-title":"Oncotarget"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1021\/acsinfecdis.6b00116","article-title":"Cell-Based High-Throughput Screening Assay Identifies 2\u2032,2\u2032-Difluoro-2\u2032-deoxycytidine Gemcitabine as a Potential Antipoliovirus Agent","volume":"3","author":"Zhang","year":"2017","journal-title":"ACS Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4089","DOI":"10.1016\/S0021-9258(18)93283-7","article-title":"Inhibitors of protein biosynthesis. V. Effects of emetine on protein and nucleic acid biosynthesis in HeLa cells","volume":"243","author":"Grollman","year":"1968","journal-title":"J. Biol. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1021\/jm00178a004","article-title":"Synthesis of syn and anti isomers of 6-[[(hydroxyimino)phenyl]methyl]-1-[(1-methylethyl)sulfonyl]-1H-benzimidaz ol-2-amine. Inhibitors of rhinovirus multiplication","volume":"23","author":"Wikel","year":"1980","journal-title":"J. Med. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"4189","DOI":"10.1128\/jvi.69.7.4189-4197.1995","article-title":"The antiviral compound enviroxime targets the 3A coding region of rhinovirus and poliovirus","volume":"69","author":"Heinz","year":"1995","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1016\/j.cell.2010.03.050","article-title":"Viral reorganization of the secretory pathway generates distinct organelles for RNA replication","volume":"141","author":"Hsu","year":"2010","journal-title":"Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2364","DOI":"10.1128\/JVI.02249-10","article-title":"Phosphatidylinositol 4-kinase III beta is a target of enviroxime-like compounds for antipoliovirus activity","volume":"85","author":"Arita","year":"2011","journal-title":"J. Virol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1016\/j.bcp.2012.07.034","article-title":"The role of phosphatidylinositol 4-kinases and phosphatidylinositol 4-phosphate during viral replication","volume":"84","author":"Delang","year":"2012","journal-title":"Biochem. Pharmacol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1021\/ml400095m","article-title":"Identification of a series of compounds with potent antiviral activity for the treatment of enterovirus infections","volume":"4","author":"MacLeod","year":"2013","journal-title":"ACS Med. Chem. Lett."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"3767","DOI":"10.1021\/acs.jmedchem.5b00499","article-title":"Highly Selective Phosphatidylinositol 4-Kinase IIIbeta Inhibitors and Structural Insight into Their Mode of Action","volume":"58","author":"Mejdrova","year":"2015","journal-title":"J. Med. Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1021\/acsinfecdis.7b00053","article-title":"Allosteric Regulation of Phosphatidylinositol 4-Kinase III Beta by an Antipicornavirus Compound MDL-860","volume":"3","author":"Arita","year":"2017","journal-title":"ACS Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1850","DOI":"10.1128\/AAC.00934-08","article-title":"Mutations in the non-structural protein 3A confer resistance to the novel enterovirus replication inhibitor TTP-8307","volume":"53","author":"Thibaut","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1099\/vir.0.023374-0","article-title":"A bifunctional anti-enterovirus compound that inhibits replication and early stage of enterovirus 71 infection","volume":"91","author":"Arita","year":"2010","journal-title":"J. Gen. Virol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"4252","DOI":"10.1128\/JVI.03546-12","article-title":"Oxysterol-binding protein family I is the target of minor enviroxime-like compounds","volume":"87","author":"Arita","year":"2013","journal-title":"J. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1016\/j.celrep.2014.12.054","article-title":"Itraconazole Inhibits Enterovirus Replication by Targeting the Oxysterol-Binding Protein","volume":"10","author":"Strating","year":"2015","journal-title":"Cell Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.antiviral.2017.01.008","article-title":"Uncovering oxysterol-binding protein (OSBP) as a target of the anti-enteroviral compound TTP-8307","volume":"140","author":"Albulescu","year":"2017","journal-title":"Antiviral Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1056\/NEJM199909233411301","article-title":"An epidemic of enterovirus 71 infection in Taiwan","volume":"341","author":"Ho","year":"1999","journal-title":"N. Engl. J. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"30226","DOI":"10.2807\/1560-7917.ES.2016.21.19.30226","article-title":"Epidemiological and clinical characteristics of patients infected with enterovirus D68, France, July to December 2014","volume":"21","author":"Schuffenecker","year":"2016","journal-title":"Eurosurveillance"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2291","DOI":"10.1021\/acsinfecdis.0c00404","article-title":"Characterization of a New Antienterovirus D68 Compound Purified from Avocado","volume":"6","author":"Arita","year":"2020","journal-title":"ACS Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1021\/acsinfecdis.9b00038","article-title":"Poliovirus Evolution toward Independence from the Phosphatidylinositol-4 Kinase III beta\/Oxysterol-Binding Protein Family I Pathway","volume":"5","author":"Arita","year":"2019","journal-title":"ACS Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3317","DOI":"10.1099\/vir.0.82172-0","article-title":"Quantitative analysis of poliomyelitis-like paralysis in mice induced by a poliovirus replicon","volume":"87","author":"Arita","year":"2006","journal-title":"J. Gen. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4441","DOI":"10.1128\/jvi.63.10.4441-4444.1989","article-title":"Guanidine-resistant mutants of poliovirus have distinct mutations in peptide 2C","volume":"63","author":"Baltera","year":"1989","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3378","DOI":"10.1128\/JVI.78.7.3378-3386.2004","article-title":"The poliovirus replication machinery can escape inhibition by an antiviral drug that targets a host cell protein","volume":"78","author":"Crotty","year":"2004","journal-title":"J. Virol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e03830","DOI":"10.7554\/eLife.03830","article-title":"Dominant drug targets suppress the emergence of antiviral resistance","volume":"3","author":"Tanner","year":"2014","journal-title":"eLife"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"8193","DOI":"10.1128\/jvi.68.12.8193-8201.1994","article-title":"Distribution of drug resistance mutations in type 3 poliovirus identifies three regions involved in uncoating functions","volume":"68","author":"Mosser","year":"1994","journal-title":"J. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"5123","DOI":"10.1016\/j.bmcl.2008.07.099","article-title":"Synthesis and anti-CVB 3 evaluation of substituted 5-nitro-2-phenoxybenzonitriles","volume":"18","author":"Purstinger","year":"2008","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2518","DOI":"10.1099\/vir.0.2008\/002915-0","article-title":"Characterization of pharmacologically active compounds that inhibit poliovirus and enterovirus 71 infectivity","volume":"89","author":"Arita","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0031-9422(02)00388-6","article-title":"Inhibition of LPS-induced NO production by the oleogum resin of Commiphora wightii and its constituents","volume":"62","author":"Meselhy","year":"2003","journal-title":"Phytochemistry"},{"key":"ref_45","first-page":"959","article-title":"Phytochemical investigation of the Australian lichens Ramalina glaucescens and Xanthoria parietina","volume":"4","author":"Dias","year":"2009","journal-title":"Nat. Prod. Commun."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"971","DOI":"10.5012\/bkcs.2010.31.04.971","article-title":"Syntheses of Resveratrol and its Hydroxylated Derivatives as Radical Scavenger and Tyrosinase Inhibitor","volume":"31","author":"Lee","year":"2010","journal-title":"Bull. Korean Chem. Soc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1002\/mrc.1583","article-title":"Comparison of the phytoestrogen trans-resveratrol (3,4\u2032,5-trihydroxystilbene) structures from x-ray diffraction and solution NMR","volume":"43","author":"Commodari","year":"2005","journal-title":"Magn. Reson. Chem."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/0031-9422(92)80078-S","article-title":"Cytotoxic anthraquinones from Rheum pulmatum","volume":"31","author":"Kubo","year":"1992","journal-title":"Phytochemistry"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1007\/s10600-009-9352-8","article-title":"A new stilbene glycoside from the n-butanol fraction of Veratrum dahuricum","volume":"45","author":"Dai","year":"2009","journal-title":"Chem. Nat. Compd."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1248\/bpb.31.1809","article-title":"Inhibition of Human Low Density Lipoprotein and High Density Lipoprotein Oxidation by Oligostilbenes from Rhubarb","volume":"31","author":"Ngoc","year":"2008","journal-title":"Biol. Pharm. Bull."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"5488","DOI":"10.1021\/jf030227o","article-title":"Delta-viniferin, a resveratrol dehydrodimer: One of the major stilbenes synthesized by stressed grapevine leaves","volume":"51","author":"Pezet","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0166-3542(01)00125-5","article-title":"In vitro antiviral activity of the anthraquinone chrysophanic acid against poliovirus","volume":"49","author":"Semple","year":"2001","journal-title":"Antiviral Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1007\/s12272-001-2133-y","article-title":"New phenylpropanoid esters of sucrose from Polygonum hydropiper and their antioxidant activity","volume":"31","author":"Kiem","year":"2008","journal-title":"Arch. Pharm. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1083\/jcb.116.2.307","article-title":"Translocation of oxysterol binding protein to Golgi apparatus triggered by ligand binding","volume":"116","author":"Ridgway","year":"1992","journal-title":"J. Cell Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.1021\/acsinfecdis.2c00108","article-title":"Ligand Recognition by the Lipid Transfer Domain of Human OSBP Is Important for Enterovirus Replication","volume":"8","author":"Kobayashi","year":"2022","journal-title":"ACS Infect. Dis."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1128\/AAC.22.4.635","article-title":"Antiviral activity and mechanism of action of 2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile (MDL-860)","volume":"22","author":"Torney","year":"1982","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1128\/AAC.22.4.611","article-title":"Antipicornavirus flavone Ro 09-0179","volume":"22","author":"Ishitsuka","year":"1982","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"A347","DOI":"10.1016\/0166-3542(95)94930-Z","article-title":"Aporphinoid alkaloid glaucinone: A selective inhibitor of poliovirus replication","volume":"26","author":"Galabov","year":"1995","journal-title":"Antiviral. Res."},{"key":"ref_59","first-page":"22","article-title":"Oxoglaucin\u2014Enterovirus replication inhibitor: Study on the antiviral spectrum, mode of action and development of resistance (in Bulgarian)","volume":"120","author":"Philipov","year":"2007","journal-title":"J. Bulg. Acad. Sci."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Wang, J., Zhang, T., Du, J., Cui, S., Yang, F., and Jin, Q. (2014). Anti-enterovirus 71 effects of chrysin and its phosphate ester. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0089668"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"eaar5759","DOI":"10.1126\/scitranslmed.aar5759","article-title":"Prunin suppresses viral IRES activity and is a potential candidate for treating enterovirus A71 infection","volume":"11","author":"Gunaseelan","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1042\/BJ20150419","article-title":"Emodin inhibits coxsackievirus B3 replication via multiple signalling cascades leading to suppression of translation","volume":"473","author":"Zhang","year":"2016","journal-title":"Biochem. J."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1038\/aps.2016.110","article-title":"Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment","volume":"38","author":"Zhong","year":"2017","journal-title":"Acta Pharmacol. Sin."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1128\/JVI.73.1.427-435.1999","article-title":"Functional coupling between replication and packaging of poliovirus replicon RNA","volume":"73","author":"Nugent","year":"1999","journal-title":"J. Virol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e0080021","DOI":"10.1128\/Spectrum.00800-21","article-title":"High-Order Epistasis and Functional Coupling of Infection Steps Drive Virus Evolution toward Independence from a Host Pathway","volume":"9","author":"Arita","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1220","DOI":"10.1128\/AAC.41.6.1220","article-title":"SCH 48973: A potent, broad-spectrum, antienterovirus compound","volume":"41","author":"Buontempo","year":"1997","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1128\/AAC.00671-09","article-title":"In vitro antiviral activity of V-073 against polioviruses","volume":"53","author":"Oberste","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"2267","DOI":"10.1128\/AAC.49.6.2267-2275.2005","article-title":"In vitro antiviral activity and single-dose pharmacokinetics in humans of a novel, orally bioavailable inhibitor of human rhinovirus 3C protease","volume":"49","author":"Patick","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.antiviral.2013.03.003","article-title":"Anti-poliovirus activity of protease inhibitor AG-7404, and assessment of in vitro activity in combination with antiviral capsid inhibitor compounds","volume":"98","author":"Rhoden","year":"2013","journal-title":"Antiviral Res."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"e0102921","DOI":"10.1128\/AAC.01029-21","article-title":"A Phase 1 Study of the Safety, Tolerability, and Pharmacokinetics of Single and Multiple Oral Doses of V-7404 in Healthy Adult Volunteers","volume":"65","author":"Kankam","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"S447","DOI":"10.1093\/infdis\/jiu043","article-title":"Progress in the development of poliovirus antiviral agents and their essential role in reducing risks that threaten eradication","volume":"210","author":"McKinlay","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_72","unstructured":"(2023, February 27). WHO. Available online: https:\/\/polioeradication.org\/tools-and-library\/current-research-areas\/antivirals\/."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1021\/np50104a007","article-title":"Vanicosides A and B, protein kinase C inhibitors from Polygonum pensylvanicum","volume":"57","author":"Zimmermann","year":"1994","journal-title":"J. Nat. Prod."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"4202","DOI":"10.1038\/s41598-018-22485-5","article-title":"Polygonumins A, a newly isolated compound from the stem of Polygonum minus Huds with potential medicinal activities","volume":"8","author":"Ahmad","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Nawrot-Hadzik, I., Zmudzinski, M., Matkowski, A., Preissner, R., Kesik-Brodacka, M., Hadzik, J., Drag, M., and Abel, R. (2021). Reynoutria Rhizomes as a Natural Source of SARS-CoV-2 Mpro Inhibitors-Molecular Docking and In Vitro Study. Pharmaceuticals, 14.","DOI":"10.3390\/ph14080742"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1111\/1348-0421.12144","article-title":"Phosphatidylinositol-4 kinase III beta and oxysterol-binding protein accumulate unesterified cholesterol on poliovirus-induced membrane structure","volume":"58","author":"Arita","year":"2014","journal-title":"Microbiol. Immunol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1021\/acsinfecdis.5b00122","article-title":"Mechanism of Poliovirus Resistance to Host Phosphatidylinositol-4 Kinase III \u03b2 Inhibitor","volume":"2","author":"Arita","year":"2016","journal-title":"ACS Infect. Dis."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.antiviral.2017.10.006","article-title":"Modulation of proteolytic polyprotein processing by coxsackievirus mutants resistant to inhibitors targeting phosphatidylinositol-4-kinase IIIbeta or oxysterol binding protein","volume":"147","author":"Lyoo","year":"2017","journal-title":"Antiviral Res."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1016\/j.celrep.2017.09.068","article-title":"Escaping Host Factor PI4KB Inhibition: Enterovirus Genomic RNA Replication in the Absence of Replication Organelles","volume":"21","author":"Melia","year":"2017","journal-title":"Cell Rep."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"5149","DOI":"10.1128\/AAC.00946-12","article-title":"Anti-hepatitis C virus activity and toxicity of type III phosphatidylinositol-4-kinase beta inhibitors","volume":"56","author":"Lamarche","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"3358","DOI":"10.1128\/AAC.00303-13","article-title":"Phosphatidylinositol 4-Kinase III Beta Is Essential for Replication of Human Rhinovirus and Its Inhibition Causes a Lethal Phenotype In Vivo","volume":"57","author":"Spickler","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"S82","DOI":"10.1093\/ofid\/ofab466.134","article-title":"134. KRP-A218, an Orally Active and Selective PI4KB Inhibitor with Broad-Spectrum Anti-Rhinovirus Activity, Has Potent Therapeutic Antiviral Activity In Vivo","volume":"8","author":"Matsui","year":"2021","journal-title":"Open Forum Infect. Dis."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/j.cell.2006.03.035","article-title":"A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling","volume":"125","author":"Knight","year":"2006","journal-title":"Cell"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/4\/903\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:07:38Z","timestamp":1760123258000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/4\/903"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,31]]},"references-count":83,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["v15040903"],"URL":"https:\/\/doi.org\/10.3390\/v15040903","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,31]]}}}