{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:50:42Z","timestamp":1788191442756,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,31]],"date-time":"2023-03-31T00:00:00Z","timestamp":1680220800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institut Pasteur de Dakar","award":["1106"],"award-info":[{"award-number":["1106"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In Senegal, the burden of dengue is increasing and expanding. As case management and traditional diagnostic techniques can be difficult to implement, rapid diagnostic tests (RDTs) deployed at point of care are ideal for investigating active outbreaks. The aim of this study was to evaluate the diagnostic performance of the Dengue NS1 and Dengue IgM\/IgG RDTs on the serum\/plasma samples in a laboratory setting and in the field. During laboratory evaluation, performance of the NS1 RDT was assessed using NS1 ELISA as the gold standard. Sensitivity and specificity were 88% [75\u201395%] and 100% [97\u2013100%], respectively. Performance of the IgM\/IG RDT was assessed using the IgM Antibody Capture (MAC) ELISA, indirect IgG, and PRNT as gold standards. The IgM and IgG test lines respectively displayed sensitivities of 94% [83\u201399%] and 70% [59\u201379%] and specificities of 91% [84\u201395%] and 91% [79\u201398%]. In the field, the Dengue NS1 RDT sensitivity and specificity was 82% [60\u201395%] and 75% [53\u201390%], respectively. The IgM and IgG test lines displayed sensitivities of 86% [42\u2013100%] and 78% [64\u201388%], specificities of 85% [76\u201392%] and 55% [36\u201373%], respectively. These results demonstrate that RDTs are ideal for use in a context of high prevalence or outbreak setting and can be implemented in the absence of a confirmatory test for acute and convalescent patients.<\/jats:p>","DOI":"10.3390\/v15040904","type":"journal-article","created":{"date-parts":[[2023,4,3]],"date-time":"2023-04-03T03:32:59Z","timestamp":1680492779000},"page":"904","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Laboratory Evaluation and Field Testing of Dengue NS1 and IgM\/IgG Rapid Diagnostic Tests in an Epidemic Context in Senegal"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0436-6240","authenticated-orcid":false,"given":"Oumar","family":"Ndiaye","sequence":"first","affiliation":[{"name":"Diatropix Unit, Institut Pasteur de Dakar, Dakar BP 220, Senegal"},{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Woolston","sequence":"additional","affiliation":[{"name":"Global Access Diagnostics, Bedford Technology Park, Bedford MK44 2YA, Bedfordshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8550-7737","authenticated-orcid":false,"given":"Aboubacry","family":"Gaye","sequence":"additional","affiliation":[{"name":"Epidemiology Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9582-4197","authenticated-orcid":false,"given":"Cheikh","family":"Loucoubar","sequence":"additional","affiliation":[{"name":"Epidemiology Clinical Research and Data Science Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Cocozza","sequence":"additional","affiliation":[{"name":"Global Access Diagnostics, Bedford Technology Park, Bedford MK44 2YA, Bedfordshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5405-2824","authenticated-orcid":false,"given":"Cheikh","family":"Fall","sequence":"additional","affiliation":[{"name":"Microbiology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatou","family":"Dia","sequence":"additional","affiliation":[{"name":"Diatropix Unit, Institut Pasteur de Dakar, Dakar BP 220, Senegal"},{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily R.","family":"Adams","sequence":"additional","affiliation":[{"name":"Global Access Diagnostics, Bedford Technology Park, Bedford MK44 2YA, Bedfordshire, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u00e8me","family":"Samb","sequence":"additional","affiliation":[{"name":"Diatropix Unit, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diogop","family":"Camara","sequence":"additional","affiliation":[{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bacary Djilocalisse","family":"Sadio","sequence":"additional","affiliation":[{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3499-4934","authenticated-orcid":false,"given":"Cheikh T.","family":"Diagne","sequence":"additional","affiliation":[{"name":"Mivegec Infectious Diseases and Vector: Ecology, Genetics, Evolution and Control, Universit\u00e9 Montpellier, IRD, CNRS, 34394 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7063-7491","authenticated-orcid":false,"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Virology, Medical School Brandenburg Theodor Fontane, D-01968 Senftenberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3478-1322","authenticated-orcid":false,"given":"Oumar","family":"Faye","sequence":"additional","affiliation":[{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph R. A.","family":"Fitchett","sequence":"additional","affiliation":[{"name":"Delivery Unit, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amadou Alpha","family":"Sall","sequence":"additional","affiliation":[{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheikh Tidiane","family":"Diagne","sequence":"additional","affiliation":[{"name":"Diatropix Unit, Institut Pasteur de Dakar, Dakar BP 220, Senegal"},{"name":"Virology Department, Institut Pasteur de Dakar, Dakar BP 220, Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4103\/2231-0762.175416","article-title":"Dengue Virus: A Global Human Threat: Review of Literature","volume":"6","author":"Hasan","year":"2016","journal-title":"J. Int. Soc. Prevent. Communit. Dent."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1186\/s13071-018-2643-9","article-title":"Natural Vertical Transmission of Dengue Virus in Aedes Aegypti and Aedes Albopictus: A Systematic Review","volume":"11","year":"2018","journal-title":"Parasites Vectors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1086\/511825","article-title":"Natural History of Dengue Virus (DENV)\u20131 and DENV-4 Infections: Reanalysis of Classic Studies","volume":"195","author":"Nishiura","year":"2007","journal-title":"J. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1708-8305.2012.00628.x","article-title":"Differential Diagnosis of Dengue Fever: Beware of Measles!: Table 1","volume":"19","author":"Pull","year":"2012","journal-title":"J. Travel Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1056\/NEJMra1110265","article-title":"Dengue","volume":"366","author":"Simmons","year":"2012","journal-title":"N. Engl. J. Med."},{"key":"ref_6","first-page":"1","article-title":"Epidemiological Surveillance of Dengue Fever: An Overview","volume":"5","author":"Kathiriya","year":"2020","journal-title":"Int. J. Vet. Sci. Amin. Hasb."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3389\/fcimb.2017.00317","article-title":"Global Epidemiology of Dengue Outbreaks in 1990\u20132015: A Systematic Review and Meta-Analysis","volume":"7","author":"Guo","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"999","DOI":"10.4269\/ajtmh.14-0628","article-title":"Dengue Serotype-Specific Differences in Clinical Manifestation, Laboratory Parameters and Risk of Severe Disease in Adults, Singapore","volume":"92","author":"Yung","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6803098","DOI":"10.1155\/2016\/6803098","article-title":"Dengue Fever: Causes, Complications, and Vaccine Strategies","volume":"2016","author":"Khetarpal","year":"2016","journal-title":"J. Immunol. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1126\/science.342.6157.415","article-title":"Tropical Medicine. Surprising New Dengue Virus Throws a Spanner in Disease Control Efforts","volume":"342","author":"Normile","year":"2013","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1086\/315215","article-title":"Dengue Viremia Titer, Antibody Response Pattern, and Virus Serotype Correlate with Disease Severity","volume":"181","author":"Vaughn","year":"2000","journal-title":"J. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1128\/microbiolspec.AID-0022-2014","article-title":"Dengue Antibody-Dependent Enhancement: Knowns and Unknowns","volume":"2","author":"Halstead","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1007\/s00705-013-1645-3","article-title":"Secondary Infection as a Risk Factor for Dengue Hemorrhagic Fever\/Dengue Shock Syndrome: An Historical Perspective and Role of Antibody-Dependent Enhancement of Infection","volume":"158","author":"Guzman","year":"2013","journal-title":"Arch. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0769-2617(80)90029-5","article-title":"Isolement du virus de la dengue au S\u00e9n\u00e9gal","volume":"131","author":"Robin","year":"1980","journal-title":"Ann. De L\u2019institut Pasteur\/Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0035-9203(86)90182-3","article-title":"Isolation of Dengue 2 and Dengue 4 Viruses from Patients in Senegal","volume":"80","author":"Saluzzo","year":"1986","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1093\/jmedent\/31.4.623","article-title":"Dengue 2 Outbreak in Southeastern Senegal during 1990: Virus Isolations from Mosquitoes (Diptera: Culicidae)","volume":"31","author":"Zeller","year":"1994","journal-title":"J. Med. Entomol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"456","DOI":"10.3201\/eid2003.121885","article-title":"Urban Epidemic of Dengue Virus Serotype 3 Infection, Senegal, 2009","volume":"20","author":"Faye","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Dieng, I., dos Passos Cunha, M., Diagne, M.M., Semb\u00e8ne, P.M., de Andrade Zanotto, P.M., Faye, O., Faye, O., and Sall, A.A. (2021). Origin and Spread of the Dengue Virus Type 1, Genotype V in Senegal, 2015\u20132019. Viruses, 13.","DOI":"10.3390\/v13010057"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"tay123","DOI":"10.1093\/jtm\/tay123","article-title":"Dengue Epidemic in Touba, Senegal: Implications for the Grand Magal Pilgrimage for Travellers","volume":"26","author":"Diagne","year":"2019","journal-title":"J. Travel Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"10321","DOI":"10.1038\/s41598-021-89070-1","article-title":"Genomic Investigation of a Dengue Virus Outbreak in Thi\u00e8s, Senegal, in 2018","volume":"11","author":"Gaye","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1086\/499282","article-title":"Antibody-Dependent Enhancement in Dengue Virus Infections","volume":"193","author":"Burke","year":"2006","journal-title":"J. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1586\/eri.10.53","article-title":"Dengue Fever: Diagnosis and Treatment","volume":"8","author":"Wiwanitkit","year":"2010","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_23","unstructured":"World Health Organization (2011). Regional Office for South-East Asia. Comprehensive Guideline for Prevention and Control of Dengue and Dengue Haemorrhagic Fever. Revised and Expanded Edition, WHO Regional Office for South-East Asia."},{"key":"ref_24","first-page":"129","article-title":"Dengue Vaccine Candidates in Development","volume":"338","author":"Durbin","year":"2010","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1549930","DOI":"10.1080\/16549716.2018.1549930","article-title":"Novel Tools for the Surveillance and Control of Dengue: Findings by the DengueTools Research Consortium","volume":"11","author":"Tissera","year":"2018","journal-title":"Glob. Health Action"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"S30","DOI":"10.1038\/nrmicro2459","article-title":"Evaluation of Diagnostic Tests: Dengue","volume":"8","author":"Peeling","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_27","unstructured":"(2021, December 26). Magnitude of Antibody Cross-Reactivity in Medically Important Mosquito-Borne Flaviviruses: A Systematic Review. Available online: https:\/\/www.tandfonline.com\/doi\/epdf\/10.2147\/IDR.S336351?needAccess=true&role=button."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"436","DOI":"10.3201\/eid1503.080923","article-title":"Evaluation of Commercially Available Anti\u2013Dengue Virus Immunoglobulin M Tests","volume":"15","author":"Hunsperger","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"S89","DOI":"10.1093\/infdis\/jiw649","article-title":"Clinical and Laboratory Diagnosis of Dengue Virus Infection","volume":"215","author":"Muller","year":"2017","journal-title":"J. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/S1386-6532(01)00250-5","article-title":"Virus Isolation as One of the Diagnostic Methods for Dengue Virus Infection","volume":"24","author":"Yamada","year":"2002","journal-title":"J. Clin. Virol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/j.diagmicrobio.2005.04.013","article-title":"Molecular Detection and Serotypic Characterization of Dengue Viruses by Single-Tube Multiplex Reverse Transcriptase-Polymerase Chain Reaction","volume":"52","author":"Kumaria","year":"2005","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jviromet.2006.11.001","article-title":"Evaluation of a Commercial Dengue NS1 Antigen-Capture ELISA for Laboratory Diagnosis of Acute Dengue Virus Infection","volume":"140","author":"Kumarasamy","year":"2007","journal-title":"J. Virol. Methods"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1922","DOI":"10.1128\/JCM.39.5.1922-1927.2001","article-title":"Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription-PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene Sequences","volume":"39","author":"Scaramozzino","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1128\/JCM.38.5.1823-1826.2000","article-title":"Standardization of Immunoglobulin M Capture Enzyme-Linked Immunosorbent Assays for Routine Diagnosis of Arboviral Infections","volume":"38","author":"Martin","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Emmerich, P., Mika, A., von Possel, R., Rackow, A., Liu, Y., Schmitz, H., G\u00fcnther, S., Sherifi, K., Halili, B., and Jakupi, X. (2018). Sensitive and Specific Detection of Crimean-Congo Hemorrhagic Fever Virus (CCHFV)\u2014Specific IgM and IgG Antibodies in Human Sera Using Recombinant CCHFV Nucleoprotein as Antigen in \u03bc-Capture and IgG Immune Complex (IC) ELISA Tests. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006366"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s100960000318","article-title":"Influence of Rheumatoid Factor on the Specificity of a Rapid Immunochromatographic Test for Diagnosing Dengue Infection","volume":"19","author":"Jelinek","year":"2000","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.3389\/fimmu.2018.02180","article-title":"Flavivirus Receptors: Diversity, Identity, and Cell Entry","volume":"9","author":"Laureti","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1111\/1469-0691.12154","article-title":"Rapid Diagnostic Tests for Non-Malarial Febrile Illness in the Tropics","volume":"19","author":"Chappuis","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_39","first-page":"38","article-title":"Catching Dengue Early: Clinical Features and Laboratory Markers of Dengue Virus Infection","volume":"63","author":"Babaliche","year":"2015","journal-title":"J. Assoc. Physicians India"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1128\/CDLI.11.4.642-650.2004","article-title":"Current Advances in Dengue Diagnosis","volume":"11","author":"Shu","year":"2004","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"eabd5393","DOI":"10.1126\/sciadv.abd5393","article-title":"Test Sensitivity Is Secondary to Frequency and Turnaround Time for COVID-19 Screening","volume":"7","author":"Larremore","year":"2021","journal-title":"Sci. Adv."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jang, W.S., Kwak, S.Y., May, W.L., Yang, D.J., Nam, J., and Lim, C.S. (2019). Comparative Evaluation of Three Dengue Duo Rapid Test Kits to Detect NS1, IgM, and IgG Associated with Acute Dengue in Children in Myanmar. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0213451"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1128\/CVI.05285-11","article-title":"Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: The Need for Combining NS1 Antigen and IgM\/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy","volume":"18","author":"Blacksell","year":"2011","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.apjtm.2016.12.002","article-title":"Serodiagnosis of Asymptomatic Dengue Infection","volume":"10","author":"Chatchen","year":"2017","journal-title":"Asian Pac. J. Trop. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1590\/0037-8682-0482-2017","article-title":"Diagnostic Significance of Immunoglobulin G Avidity in Symptomatic and Asymptomatic West Nile Virus Infection","volume":"51","author":"Kristofic","year":"2018","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1111\/j.1348-0421.1994.tb02152.x","article-title":"Studies on Serological Cross-Reaction in Sequential Flavivirus Infections","volume":"38","author":"Makino","year":"1994","journal-title":"Microbiol. Immunol."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/4\/904\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:07:54Z","timestamp":1760123274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/4\/904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,31]]},"references-count":46,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["v15040904"],"URL":"https:\/\/doi.org\/10.3390\/v15040904","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,31]]}}}