{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T01:16:43Z","timestamp":1767835003761,"version":"3.49.0"},"reference-count":21,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,4,30]],"date-time":"2023-04-30T00:00:00Z","timestamp":1682812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Cases of mpox have been reported in several European countries, including Spain. Our objective was to evaluate the usefulness of serum and nasopharyngeal samples for diagnosis of mpox. The presence of MPXV DNA was studied using real-time PCR (CerTest Biotec, Zaragoza, Spain) in 106 samples from 50 patients: 32 skin, 31 anogenital, 25 sera, and 18 nasopharyngeal\/pharyngeal, in the Hospital Cl\u00ednico Universitario of Zaragoza (Spain). Sixty-three samples from twenty-seven patients were MPXV PCR-positive. The real-time PCR Ct values in the anogenital and skin samples were lower than serum and nasopharyngeal samples. More than 90% of anogenital (95.7%), serum (94.4%), and skin (92.9%) samples were real-time PCR-positive. Eighteen (66.7%) of the twenty-seven patients who were MPXV PCR-positive had antecedents or presented with one to three sexually transmitted infection (STI) agents. Our results indicate that the use of serum samples can help facilitate the diagnosis of MPXV infections.<\/jats:p>","DOI":"10.3390\/v15051107","type":"journal-article","created":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T12:02:31Z","timestamp":1682942551000},"page":"1107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Usefulness of Non-Skin Samples in the PCR Diagnosis of Mpox (Monkeypox)"],"prefix":"10.3390","volume":"15","author":[{"given":"Sonia","family":"Algarate","sequence":"first","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, Microbiology Department, University of Zaragoza, Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Bueno","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda J.","family":"Crusells","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, Microbiology Department, University of Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariano","family":"Ara","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, Microbiology Department, University of Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henar","family":"Alonso","sequence":"additional","affiliation":[{"name":"Microbiology Department, University of Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Alvarado","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Ducons","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Arnal","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5134-1006","authenticated-orcid":false,"given":"Rafael","family":"Benito","sequence":"additional","affiliation":[{"name":"Hospital Cl\u00ednico Universitario Lozano Blesa de Zaragoza, Microbiology Department, University of Zaragoza, Instituto de Investigaci\u00f3n Sanitaria de Arag\u00f3n, 50009 Zaragoza, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,30]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2022, December 15). WHO Recommends New Name for Monkeypox Disease, Available online: https:\/\/www.who.int\/europe\/news\/item\/28-11-2022-who-recommends-new-name-for-monkeypox-disease."},{"key":"ref_2","unstructured":"European Centre for Disease Prevention and Control (ECDPC) (2022, September 14). Monkeypox Multi-Country Outbreak. May 2022, Available online: https:\/\/www.ecdc.europa.eu\/sites\/default\/files\/documents\/Monkeypox-multi-country-outbreak.pdf."},{"key":"ref_3","unstructured":"Ministry of Health (2023, January 05). Situation Report in Spain and Other Non-Endemic Countries, Available online: http:\/\/www.sanidad.gob.es\/profesionales\/saludPublica\/ccayes\/alertasActual\/alertaMonkeypox\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/j.jviromet.2010.07.012","article-title":"Real-time PCR assays for the specific detection of monkeypox virus West African and Congo Basin strain DNA","volume":"169","author":"Li","year":"2010","journal-title":"J. Virol. 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