{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:23:18Z","timestamp":1790738598222,"version":"4.1.0"},"reference-count":38,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key Research and Development Program of China","award":["2022YFC2303300"],"award-info":[{"award-number":["2022YFC2303300"]}]},{"name":"National Key Research and Development Program of China","award":["JXBS001"],"award-info":[{"award-number":["JXBS001"]}]},{"name":"National Key Research and Development Program of China","award":["31900146"],"award-info":[{"award-number":["31900146"]}]},{"name":"National Key Research and Development Program of China","award":["2021CFA050"],"award-info":[{"award-number":["2021CFA050"]}]},{"name":"Key Biosafety Science and Technology Program of Hubei Jiangxia Laboratory","award":["2022YFC2303300"],"award-info":[{"award-number":["2022YFC2303300"]}]},{"name":"Key Biosafety Science and Technology Program of Hubei Jiangxia Laboratory","award":["JXBS001"],"award-info":[{"award-number":["JXBS001"]}]},{"name":"Key Biosafety Science and Technology Program of Hubei Jiangxia Laboratory","award":["31900146"],"award-info":[{"award-number":["31900146"]}]},{"name":"Key Biosafety Science and Technology Program of Hubei Jiangxia Laboratory","award":["2021CFA050"],"award-info":[{"award-number":["2021CFA050"]}]},{"name":"National Natural Science Foundation of China","award":["2022YFC2303300"],"award-info":[{"award-number":["2022YFC2303300"]}]},{"name":"National Natural Science Foundation of China","award":["JXBS001"],"award-info":[{"award-number":["JXBS001"]}]},{"name":"National Natural Science Foundation of China","award":["31900146"],"award-info":[{"award-number":["31900146"]}]},{"name":"National Natural Science Foundation of China","award":["2021CFA050"],"award-info":[{"award-number":["2021CFA050"]}]},{"name":"Hubei Natural Science Foundation for Distinguished Young Scholars","award":["2022YFC2303300"],"award-info":[{"award-number":["2022YFC2303300"]}]},{"name":"Hubei Natural Science Foundation for Distinguished Young Scholars","award":["JXBS001"],"award-info":[{"award-number":["JXBS001"]}]},{"name":"Hubei Natural Science Foundation for Distinguished Young Scholars","award":["31900146"],"award-info":[{"award-number":["31900146"]}]},{"name":"Hubei Natural Science Foundation for Distinguished Young Scholars","award":["2021CFA050"],"award-info":[{"award-number":["2021CFA050"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Severe fever with thrombocytopenia syndrome (SFTS) caused by a novel bunyavirus (SFTSV) is an emerging infectious disease with up to 30% case fatality. Currently, there are no specific antiviral drugs or vaccines for SFTS. Here, we constructed a reporter SFTSV in which the virulent factor nonstructural protein (NSs) was replaced by eGFP for drug screening. First, we developed a reverse genetics system based on the SFTSV HBMC5 strain. Then, the reporter virus SFTSV-delNSs-eGFP was constructed, rescued, and characterized in vitro. SFTSV-delNSs-eGFP showed similar growth kinetics with the wild-type virus in Vero cells. We further detected the antiviral efficacy of favipiravir and chloroquine against wild-type and recombinant SFTSV by the quantification of viral RNA, and compared the results with that of fluorescent assay using high-content screening. The results showed that SFTSV-delNSs-eGFP could be used as a reporter virus for antiviral drug screening in vitro. In addition, we analyzed the pathogenesis of SFTSV-delNSs-eGFP in interferon receptor-deficient (IFNAR\u2212\/\u2212) C57BL\/6J mice and found that unlike the fatal infection of the wild-type virus, no obvious pathological change or viral replication were observed in SFTSV-delNSs-eGFP-infected mice. Taken together, the green fluorescence and attenuated pathogenicity make SFTSV-delNSs-eGFP a potent tool for the future high-throughput screening of antiviral drugs.<\/jats:p>","DOI":"10.3390\/v15051147","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T01:37:09Z","timestamp":1683769029000},"page":"1147","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Construction and Characterization of Severe Fever with Thrombocytopenia Syndrome Virus with a Fluorescent Reporter for Antiviral Drug Screening"],"prefix":"10.3390","volume":"15","author":[{"given":"Xiao","family":"Wang","sequence":"first","affiliation":[{"name":"Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"},{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanhuan","family":"Ke","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longda","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"},{"name":"Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liushuai","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5385-083X","authenticated-orcid":false,"given":"Fei","family":"Deng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8701-3530","authenticated-orcid":false,"given":"Manli","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"},{"name":"Hubei Jiangxia Laboratory, Wuhan 430200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1560-0928","authenticated-orcid":false,"given":"Zhihong","family":"Hu","sequence":"additional","affiliation":[{"name":"Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"},{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3513","DOI":"10.1007\/s00705-021-05143-6","article-title":"2021 Taxonomic update of phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales","volume":"166","author":"Kuhn","year":"2021","journal-title":"Arch. 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