{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:09:53Z","timestamp":1760148593589,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T00:00:00Z","timestamp":1684972800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Science and Technology Innovation Program of Shanxi Agricultural University","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"special Fund for Science and Technology Innovation Teams of Shanxi Province","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"Fund for Shanxi \u201c1331 Project\u201d","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"University Science and Technology Innovation Project of Shanxi Province","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"Scientific Research Project of Shanxi Province Doctoral Graduates and Postdoctoral Researchers to Work in Shanxi Province","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"Innovation Projects of the College of Veterinary Medicine, Shanxi Agricultural University","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"Shanxi Key Laboratory of Protein Structure Determination","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]},{"name":"Scientific Research Project of Colleges and Universities in Henan Province","award":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"],"award-info":[{"award-number":["32202786","2020BQ59","202204051001022","20211331-13","2021L145","SXBYKY2021036","DY-Q008","202104010910006","22B230001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Pseudorabies virus (PRV) variants have caused substantial economic losses in the swine industry in China since 2011. To surveil the genetic variation in PRV field strains, here, two novel variant strains of PRV were isolated from Shanxi Province in central China and were designated SX1910 and SX1911. To identify the genetic characteristics of the two isolates, their complete genomes were sequenced, and phylogenetic analysis and sequence alignment revealed that field PRV variants have undergone genetic variations; notably, the protein-coding sequences UL5, UL36, US1 and IE180 exhibited extensive variation and contained one or more hypervariable regions. Furthermore, we also found that the glycoproteins gB and gD of the two isolates had some novel amino acid (aa) mutations. Importantly, most of these mutations were located on the surface of the protein molecule, according to protein structure model analysis. We constructed a mutant virus of SX1911 with deletion of the gE and gI genes via CRISPR\/Cas9. When tested in mice, SX1911-\u0394gE\/gI-vaccinated mice were protected within a comparable range to Bartha-K61-vaccinated mice. Additionally, a higher dose of inactivated Bartha-K61 protected the mice from lethal SX1911 challenge, while a lower neutralization titer, higher viral load and more severe microscopic lesions were displayed in Bartha-K61-vaccinated mice. These findings highlight the need for continuous monitoring of PRV and novel vaccine development or vaccination program design for PRV control in China.<\/jats:p>","DOI":"10.3390\/v15061237","type":"journal-article","created":{"date-parts":[[2023,5,25]],"date-time":"2023-05-25T02:30:06Z","timestamp":1684981806000},"page":"1237","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genomic Characterization and gE\/gI-Deleted Strain Construction of Novel PRV Variants Isolated in Central China"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8362-6564","authenticated-orcid":false,"given":"Jianle","family":"Ren","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinxin","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Solarbio Science & Technology Co., Ltd., Beijing 101102, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiying","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhihong","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3520-1270","authenticated-orcid":false,"given":"Lei","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Food Engineering, Anyang Institute of Technology, Anyang 455000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolong","family":"Gao","sequence":"additional","affiliation":[{"name":"Beijing Animal Disease Prevention and Control Center, Beijing 102629, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Niu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Libin","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junping","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yujun","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-xia","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,25]]},"reference":[{"key":"ref_1","first-page":"99","article-title":"Aujeszky\u2019s disease (pseudorabies) virus: The virus and molecular pathogenesis\u2014state of the art, June 1999","volume":"31","author":"Mettenleiter","year":"2000","journal-title":"Vet. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/MMBR.69.3.462-500.2005","article-title":"Molecular biology of pseudorabies virus: Impact on neurovirology and veterinary medicine","volume":"69","author":"Pomeranz","year":"2005","journal-title":"Microbiol. Mol. Biol. Rev. MMBR"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1691","DOI":"10.1007\/s00705-011-1080-2","article-title":"Pseudorabies virus in wild swine: A global perspective","volume":"156","author":"Muller","year":"2011","journal-title":"Arch. Virol."},{"key":"ref_4","first-page":"65","article-title":"A review of pseudorabies (Aujeszky\u2019s disease) in pigs","volume":"20","author":"Lee","year":"1979","journal-title":"Can. Vet. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.vetmic.2015.12.008","article-title":"Control of swine pseudorabies in China: Opportunities and limitations","volume":"183","author":"Sun","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.vetmic.2016.11.019","article-title":"Vaccines against pseudorabies virus (PrV)","volume":"206","author":"Freuling","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Mettenleiter, T.C. (2020). Aujeszky\u2019s Disease and the Development of the Marker\/DIVA Vaccination Concept. Pathogens, 9.","DOI":"10.3390\/pathogens9070563"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Laval, K., and Enquist, L.W. (2020). The Neuropathic Itch Caused by Pseudorabies Virus. Pathogens, 9.","DOI":"10.3390\/pathogens9040254"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Zheng, H.H., Fu, P.F., Chen, H.Y., and Wang, Z.Y. (2022). Pseudorabies Virus: From Pathogenesis to Prevention Strategies. Viruses, 14.","DOI":"10.3390\/v14081638"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Delva, J.L., Nauwynck, H.J., Mettenleiter, T.C., and Favoreel, H.W. (2020). The Attenuated Pseudorabies Virus Vaccine Strain Bartha K61: A Brief Review on the Knowledge Gathered during 60 Years of Research. Pathogens, 9.","DOI":"10.3390\/pathogens9110897"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1136\/vr.144.14.365","article-title":"Eradication of Aujeszky\u2019s disease from New Zealand pig herds 1976\u20131997","volume":"144","author":"Pannett","year":"1999","journal-title":"Vet. Rec."},{"key":"ref_12","first-page":"38","article-title":"Epidemic status and characteristics of swine pseudorabies [in Chinese]","volume":"33","author":"Yang","year":"2016","journal-title":"Swine Indus. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.3201\/eid1911.130177","article-title":"Pseudorabies virus variant in Bartha-K61-vaccinated pigs, China, 2012","volume":"19","author":"An","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"102","DOI":"10.3201\/eid2001.130531","article-title":"Pathogenic pseudorabies virus, China, 2012","volume":"20","author":"Yu","year":"2014","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.vetmic.2014.09.003","article-title":"Pathogenicity and genomic characterization of a pseudorabies virus variant isolated from Bartha-K61-vaccinated swine population in China","volume":"174","author":"Luo","year":"2014","journal-title":"Vet. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/j.vetmic.2015.09.021","article-title":"Emergence of a Pseudorabies virus variant with increased virulence to piglets","volume":"181","author":"Tong","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"23","DOI":"10.5501\/wjv.v5.i1.23","article-title":"Pathogenicity of a currently circulating Chinese variant pseudorabies virus in pigs","volume":"5","author":"Yang","year":"2016","journal-title":"World J. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3379","DOI":"10.1016\/j.vaccine.2014.04.035","article-title":"A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China","volume":"32","author":"Wang","year":"2014","journal-title":"Vaccine"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.virusres.2014.09.003","article-title":"A novel inactivated gE\/gI deleted pseudorabies virus (PRV) vaccine completely protects pigs from an emerged variant PRV challenge","volume":"195","author":"Gu","year":"2015","journal-title":"Virus Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5733","DOI":"10.1016\/j.vaccine.2015.09.066","article-title":"Novel pseudorabies virus variant with defects in TK, gE and gI protects growing pigs against lethal challenge","volume":"33","author":"Hu","year":"2015","journal-title":"Vaccine"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"323","DOI":"10.3389\/fmicb.2020.00323","article-title":"Glycoproteins C and D of PRV Strain HB1201 Contribute Individually to the Escape From Bartha-K61 Vaccine-Induced Immunity","volume":"11","author":"Ren","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.virol.2015.04.013","article-title":"Genomic characterization of emergent pseudorabies virus in China reveals marked sequence divergence: Evidence for the existence of two major genotypes","volume":"483","author":"Ye","year":"2015","journal-title":"Virology"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.virol.2016.01.016","article-title":"Genomic analyses reveal that partial sequence of an earlier pseudorabies virus in China is originated from a Bartha-vaccine-like strain","volume":"491","author":"Ye","year":"2016","journal-title":"Virology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.virol.2018.03.024","article-title":"Polymorphisms affecting the gE and gI proteins partly contribute to the virulence of a newly-emergent highly virulent Chinese pseudorabies virus","volume":"519","author":"Dong","year":"2018","journal-title":"Virology"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1007\/s12250-019-00140-1","article-title":"Genome Characteristics and Evolution of Pseudorabies Virus Strains in Eastern China from 2017 to 2019","volume":"34","author":"Zhai","year":"2019","journal-title":"Virol. Sin."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2274","DOI":"10.1111\/tbed.13784","article-title":"Novel Chinese pseudorabies virus variants undergo extensive recombination and rapid interspecies transmission","volume":"67","author":"Liu","year":"2020","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hu, R., Wang, L., Liu, Q., Hua, L., Huang, X., Zhang, Y., Fan, J., Chen, H., Song, W., and Liang, W. (2021). Whole-Genome Sequence Analysis of Pseudorabies Virus Clinical Isolates from Pigs in China between 2012 and 2017 in China. Viruses, 13.","DOI":"10.3390\/v13071322"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.vetmic.2019.04.031","article-title":"Commercial vaccine against pseudorabies virus: A hidden health risk for dogs","volume":"233","author":"Lin","year":"2019","journal-title":"Vet. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1007\/s13365-020-00855-y","article-title":"Pseudorabies virus encephalitis in humans: A case series study","volume":"26","author":"Fan","year":"2020","journal-title":"J. Neurovirol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1016\/j.jinf.2019.12.018","article-title":"Human pseudorabies virus infection: A new threat in China","volume":"80","author":"Ou","year":"2020","journal-title":"J. Infect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s00705-020-04848-4","article-title":"Retrospective detection and phylogenetic analysis of pseudorabies virus in dogs in China","volume":"166","author":"Tu","year":"2021","journal-title":"Arch. Virol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am. J. Epidemiol."},{"key":"ref_33","first-page":"e3151","article-title":"Preparation of viral DNA from nucleocapsids","volume":"54","author":"Szpara","year":"2011","journal-title":"J. Vis. Exp. JoVE"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1016\/0022-2836(67)90307-5","article-title":"Selective extraction of polyoma DNA from infected mouse cell cultures","volume":"26","author":"Hirt","year":"1967","journal-title":"J. Mol. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1007\/s12250-020-00340-0","article-title":"Current Status and Challenge of Pseudorabies Virus Infection in China","volume":"36","author":"Tan","year":"2021","journal-title":"Virol. Sin."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"764982","DOI":"10.3389\/fvets.2021.764982","article-title":"Isolation and Phylogenetic Analysis of Reemerging Pseudorabies Virus Within Pig Populations in Central China During 2012 to 2019","volume":"8","author":"Zheng","year":"2021","journal-title":"Front. Vet. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Bo, Z., and Li, X. (2022). A Review of Pseudorabies Virus Variants: Genomics, Vaccination, Transmission, and Zoonotic Potential. Viruses, 14.","DOI":"10.3390\/v14051003"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Liu, Q., Kuang, Y., Li, Y., Guo, H., Zhou, C., Guo, S., Tan, C., Wu, B., Chen, H., and Wang, X. (2022). The Epidemiology and Variation in Pseudorabies Virus: A Continuing Challenge to Pigs and Humans. Viruses, 14.","DOI":"10.3390\/v14071463"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"612474","DOI":"10.3389\/fmicb.2020.612474","article-title":"Implication of the Identification of an Earlier Pseudorabies Virus (PRV) Strain HLJ-2013 to the Evolution of Chinese PRVs","volume":"11","author":"Liu","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"311","DOI":"10.2217\/fmb.13.4","article-title":"HSV-1 ICP22: Hijacking host nuclear functions to enhance viral infection","volume":"8","author":"Rice","year":"2013","journal-title":"Future Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Takacs, I.F., Tombacz, D., Berta, B., Prazsak, I., Poka, N., and Boldogkoi, Z. (2013). The ICP22 protein selectively modifies the transcription of different kinetic classes of pseudorabies virus genes. BMC Mol. Biol., 14.","DOI":"10.1186\/1471-2199-14-2"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.chom.2013.01.009","article-title":"The herpesvirus VP1\/2 protein is an effector of dynein-mediated capsid transport and neuroinvasion","volume":"13","author":"Zaichick","year":"2013","journal-title":"Cell Host Microbe"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1080\/15476286.2020.1731664","article-title":"G(2)-quadruplex in the 3\u2019UTR of IE180 regulates Pseudorabies virus replication by enhancing gene expression","volume":"17","author":"Zhang","year":"2020","journal-title":"RNA Biol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2657","DOI":"10.1167\/iovs.02-1105","article-title":"Multiple determinants contribute to the virulence of HSV ocular and CNS infection and identification of serine 34 of the US1 gene as an ocular disease determinant","volume":"44","author":"Brandt","year":"2003","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1006\/viro.2002.1746","article-title":"Mutation of the protein tyrosine kinase consensus site in the herpes simplex virus 1 alpha22 gene alters ICP22 posttranslational modification","volume":"305","author":"Aubert","year":"2003","journal-title":"Virology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"13510","DOI":"10.1128\/JVI.02424-13","article-title":"Herpes simplex virus 1 ICP22 but not US 1.5 is required for efficient acute replication in mice and VICE domain formation","volume":"87","author":"Mostafa","year":"2013","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"e0114022","DOI":"10.1128\/spectrum.01140-22","article-title":"Deletion of Double Copies of the US1 Gene Reduces the Infectivity of Recombinant Duck Plague Virus In Vitro and In Vivo","volume":"10","author":"Wu","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.vetmic.2017.07.019","article-title":"Variations in glycoprotein B contribute to immunogenic difference between PRV variant JS-2012 and Bartha-K61","volume":"208","author":"Yu","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Zhang, C., Liu, Y., Chen, S., Qiao, Y., Guo, M., Zheng, Y., Xu, M., Wang, Z., Hou, J., and Wang, J. (2019). A gD&gC-substituted pseudorabies virus vaccine strain provides complete clinical protection and is helpful to prevent virus shedding against challenge by a Chinese pseudorabies variant. BMC Vet. Res., 15.","DOI":"10.1186\/s12917-018-1766-8"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.meegid.2018.12.016","article-title":"Characterization of a moderately pathogenic pseudorabies virus variant isolated in China, 2014","volume":"68","author":"Zhou","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.rvsc.2019.10.005","article-title":"Efficacy of the Bartha-K61 vaccine and a gE\u2212\/gI\u2212\/TK\u2212 prototype vaccine against variant porcine pseudorabies virus (vPRV) in piglets with sublethal challenge of vPRV","volume":"128","author":"Wang","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.vetmic.2015.11.022","article-title":"Pathogenicity and immunogenicity of a gE\/gI\/TK gene-deleted pseudorabies virus variant in susceptible animals","volume":"182","author":"Cong","year":"2016","journal-title":"Vet. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.antiviral.2016.03.002","article-title":"A live, attenuated pseudorabies virus strain JS-2012 deleted for gE\/gI protects against both classical and emerging strains","volume":"130","author":"Tong","year":"2016","journal-title":"Antivir. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.rvsc.2017.01.011","article-title":"Comparative pathogenicity and immunogenicity of triple and double gene-deletion pseudorabies virus vaccine candidates","volume":"115","author":"Dong","year":"2017","journal-title":"Res. Vet. Sci."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/6\/1237\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:41:40Z","timestamp":1760125300000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/6\/1237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,25]]},"references-count":54,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["v15061237"],"URL":"https:\/\/doi.org\/10.3390\/v15061237","relation":{},"ISSN":["1999-4915"],"issn-type":[{"type":"electronic","value":"1999-4915"}],"subject":[],"published":{"date-parts":[[2023,5,25]]}}}