{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:46:03Z","timestamp":1791517563562,"version":"4.3.4"},"reference-count":24,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T00:00:00Z","timestamp":1685404800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health USA","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"National Institutes of Health USA","award":["2021\/11944-6"],"award-info":[{"award-number":["2021\/11944-6"]}]},{"name":"National Institutes of Health USA","award":["03\/11"],"award-info":[{"award-number":["03\/11"]}]},{"name":"National Institutes of Health USA","award":["COF 03\/11"],"award-info":[{"award-number":["COF 03\/11"]}]},{"name":"United World Arbovirus Research Network (UWARN)","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"United World Arbovirus Research Network (UWARN)","award":["2021\/11944-6"],"award-info":[{"award-number":["2021\/11944-6"]}]},{"name":"United World Arbovirus Research Network (UWARN)","award":["03\/11"],"award-info":[{"award-number":["03\/11"]}]},{"name":"United World Arbovirus Research Network (UWARN)","award":["COF 03\/11"],"award-info":[{"award-number":["COF 03\/11"]}]},{"name":"PAHO, FAPESP","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"PAHO, FAPESP","award":["2021\/11944-6"],"award-info":[{"award-number":["2021\/11944-6"]}]},{"name":"PAHO, FAPESP","award":["03\/11"],"award-info":[{"award-number":["03\/11"]}]},{"name":"PAHO, FAPESP","award":["COF 03\/11"],"award-info":[{"award-number":["COF 03\/11"]}]},{"name":"Mercosur Structural Convergence Fund (FOCEM)","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"Mercosur Structural Convergence Fund (FOCEM)","award":["2021\/11944-6"],"award-info":[{"award-number":["2021\/11944-6"]}]},{"name":"Mercosur Structural Convergence Fund (FOCEM)","award":["03\/11"],"award-info":[{"award-number":["03\/11"]}]},{"name":"Mercosur Structural Convergence Fund (FOCEM)","award":["COF 03\/11"],"award-info":[{"award-number":["COF 03\/11"]}]},{"name":"Education and Biotechnologies Applied to Health","award":["U01 AI151698"],"award-info":[{"award-number":["U01 AI151698"]}]},{"name":"Education and Biotechnologies Applied to Health","award":["2021\/11944-6"],"award-info":[{"award-number":["2021\/11944-6"]}]},{"name":"Education and Biotechnologies Applied to Health","award":["03\/11"],"award-info":[{"award-number":["03\/11"]}]},{"name":"Education and Biotechnologies Applied to Health","award":["COF 03\/11"],"award-info":[{"award-number":["COF 03\/11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Dengue virus (DENV) has been a major public health concern in Paraguay, with frequent outbreaks occurring since early 1988. Although control measures have been implemented, dengue remains a significant health threat in the country, and continued efforts are required for prevention and control. In response to that, in collaboration with the Central Public Health Laboratory in Asunci\u00f3n, we conducted a portable whole-genome sequencing and phylodynamic analysis to investigate DENV viral strains circulating in Paraguay over the past epidemics. Our genomic surveillance activities revealed the co-circulation of multiple DENV serotypes: DENV-1 genotype V, the emerging DENV-2 genotype III, BR4-L2 clade, and DENV-4 genotype II. Results additionally highlight the possible role of Brazil as a source for the international dispersion of different viral strains to other countries in the Americas emphasizing the need for increased surveillance across the borders, for the early detection and response to outbreaks. This, in turn, emphasizes the critical role of genomic surveillance in monitoring and understanding arbovirus transmission and persistence locally and over long distances.<\/jats:p>","DOI":"10.3390\/v15061275","type":"journal-article","created":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T02:04:21Z","timestamp":1685412261000},"page":"1275","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Retrospective Spatio-Temporal Dynamics of Dengue Virus 1, 2 and 4 in Paraguay"],"prefix":"10.3390","volume":"15","author":[{"given":"Cynthia","family":"Vazquez","sequence":"first","affiliation":[{"name":"Laboratorio Central de Salud P\u00fablica, Asunci\u00f3n 001535, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Carlos Junior","family":"Alcantara","sequence":"additional","affiliation":[{"name":"Instituto Rene Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, MG, 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