{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:51:00Z","timestamp":1792457460722,"version":"4.5.0"},"reference-count":32,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,13]],"date-time":"2023-06-13T00:00:00Z","timestamp":1686614400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004795","name":"Institut Universitaire de France","doi-asserted-by":"publisher","award":["IUF-2017"],"award-info":[{"award-number":["IUF-2017"]}],"id":[{"id":"10.13039\/501100004795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Mycoviruses are viruses that selectively infect and multiply in fungal cells. Malassezia is the most abundant fungus on human skin and is associated with a variety of conditions, including atopic eczema, atopic dermatitis, dandruff, folliculitis, pityriasis versicolor, and seborrheic dermatitis. Here, we conducted mycovirome studies on 194 public transcriptomes of Malassezia (2,568,212,042 paired-end reads) screened against all available viral proteins. Transcriptomic data were assembled de novo resulting in 1,170,715 contigs and 2,995,306 open reading frames (ORFs) that were subsequently tracked for potential viral sequences. Eighty-eight virus-associated ORFs were detected in 68 contigs from 28 Sequence Read Archive (SRA) samples. Seventy-five and thirteen ORFs were retrieved from transcriptomes of Malassezia globosa and Malassezia restricta, respectively. Phylogenetic reconstructions revealed three new mycoviruses belonging to the Totivirus genus and named Malassezia globosa-associated-totivirus 1 (MgaTV1); Malassezia restricta-associated-totivirus 1 (MraTV1) and Malassezia restricta-associated-totivirus 2 (MraTV2). These viral candidates extend our understanding of the diversity and taxonomy of mycoviruses as well as their co-evolution with their fungal hosts. These results reflected the unexpected diversity of mycoviruses hidden in public databases. In conclusion, this study sheds light on the discovery of novel mycoviruses and opens the door to study their impact on disease caused by the host fungus Malassezia and globally, their implication in clinical skin disorders.<\/jats:p>","DOI":"10.3390\/v15061368","type":"journal-article","created":{"date-parts":[[2023,6,14]],"date-time":"2023-06-14T01:32:15Z","timestamp":1686706335000},"page":"1368","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Tracking Mycoviruses in Public RNAseq Datasets of Malassezia: Three Original Totiviruses Revealed"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6976-8168","authenticated-orcid":false,"given":"Fatima","family":"Boulanouar","sequence":"first","affiliation":[{"name":"Aix-Marseille Universit\u00e9 (AMU), UMR VITROME, IRD, APHM, Facult\u00e9 de M\u00e9decine, 13005 Marseille, France"},{"name":"Aix-Marseille Universit\u00e9 (AMU), UMR MEPHI, IRD, APHM, Facult\u00e9 de M\u00e9decine, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-5276","authenticated-orcid":false,"given":"St\u00e9phane","family":"Ranque","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9 (AMU), UMR VITROME, IRD, APHM, Facult\u00e9 de M\u00e9decine, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Levasseur","sequence":"additional","affiliation":[{"name":"Aix-Marseille Universit\u00e9 (AMU), UMR VITROME, IRD, APHM, Facult\u00e9 de M\u00e9decine, 13005 Marseille, France"},{"name":"Aix-Marseille Universit\u00e9 (AMU), UMR MEPHI, IRD, APHM, Facult\u00e9 de M\u00e9decine, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"},{"name":"Institut Universitaire de France, 75005 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,13]]},"reference":[{"key":"ref_1","first-page":"1011","article-title":"Fungal viruses: Promising fundamental research and biological control agents of fungi","volume":"2","author":"Wang","year":"2017","journal-title":"SM Virol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8387","DOI":"10.1073\/pnas.0913535107","article-title":"A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus","volume":"107","author":"Yu","year":"2010","journal-title":"Proc. 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