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Research on the pathogenesis has focused largely on effects of dengue virus (DENV) at the blood\u2013retinal barrier. Involvement of retinal M\u00fcller glial cells has received little attention, although this cell population contributes to the pathology of other intraocular infections. The goal of our work was to establish the susceptibility of M\u00fcller cells to infection with DENV and to identify characteristics of the cellular antiviral, inflammatory, and immunomodulatory responses to DENV infection in vitro. Primary human M\u00fcller cell isolates and the MIO-M1 human M\u00fcller cell line were infected with the laboratory-adapted Mon601 strain and DENV serotype 1 and 2 field isolates, and cell\u2013DENV interactions were investigated by immunolabelling and quantitative real-time polymerase chain reaction. M\u00fcller cells were susceptible to DENV infection, but experiments involving primary cell isolates indicated inter-individual variation. Viral infection induced an inflammatory response (including tumour necrosis factor-\u03b1, interleukin [IL]-1\u03b2, and IL-6) and an immunomodulatory response (including programmed death-ligand [PD-L]1 and PD-L2). The type I interferon response was muted in the M\u00fcller cell line compared to primary cell isolates. The highest infectivity and cell responses were observed in the laboratory-adapted strain, and overall, infectivity and cell responses were stronger in DENV2 strains. This work demonstrates that M\u00fcller cells mount an antiviral and immune response to DENV infection, and that this response varies across cell isolates and DENV strain. The research provides a direction for future efforts to understand the role of human retinal M\u00fcller glial cells in dengue retinopathy.<\/jats:p>","DOI":"10.3390\/v15071410","type":"journal-article","created":{"date-parts":[[2023,6,22]],"date-time":"2023-06-22T01:49:32Z","timestamp":1687398572000},"page":"1410","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Dengue Virus Infection of Human Retinal M\u00fcller Glial Cells"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-1552","authenticated-orcid":false,"given":"Genevieve F.","family":"Oliver","sequence":"first","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam M.","family":"Ashander","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abby C.","family":"Dawson","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuefang","family":"Ma","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1080-1472","authenticated-orcid":false,"given":"Jillian M.","family":"Carr","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keryn A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4756-5493","authenticated-orcid":false,"given":"Justine R.","family":"Smith","sequence":"additional","affiliation":[{"name":"Flinders Health and Medical Research Institute, and College of Medicine and Public Health, Flinders University, Adelaide, SA 5042, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,21]]},"reference":[{"key":"ref_1","first-page":"42","article-title":"Affections of the eye following dengue","volume":"8","author":"Roy","year":"1873","journal-title":"Ind. 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