{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T06:30:42Z","timestamp":1783924242308,"version":"3.55.0"},"reference-count":33,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,23]],"date-time":"2023-06-23T00:00:00Z","timestamp":1687478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Kao Corporation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly contagious and continues to spread worldwide. To avoid the spread of infection, it is important to control its transmission routes. However, as methods to prevent airborne infections are lacking, people are forced to take measures such as keeping distance from others or wearing masks. Here, we evaluate the antiviral activity of propylene glycol (PG), which is safe, odorless, and volatile. PG showed pronounced antiviral activity against the influenza virus (IAV) at concentrations above 55% in the liquid phase. Given its IAV inactivation mechanism, which involves increasing the fluidity of the viral membrane, PG is expected to have a broad effect on enveloped viruses. PG showed antiviral activity against SARS-CoV-2. We also developed a system to evaluate the antiviral effect of PG in spray and volatilized forms. PG was found to be effective against aerosol IAV in both forms; the effective PG concentration against IAV in the vapor phase was 87 ppmv (0.27 mg\/L). These results demonstrate that PG is an effective means for viral inactivation in various situations for infection control. This technology is expected to control the spread of current and future infectious diseases capable of causing outbreaks and pandemics.<\/jats:p>","DOI":"10.3390\/v15071421","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T02:05:02Z","timestamp":1687745102000},"page":"1421","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Antiviral Effect of Propylene Glycol against Envelope Viruses in Spray and Volatilized Forms"],"prefix":"10.3390","volume":"15","author":[{"given":"Yui","family":"Hirama","sequence":"first","affiliation":[{"name":"Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tokyo 321-3497, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7104-3225","authenticated-orcid":false,"given":"Shintaro","family":"Onishi","sequence":"additional","affiliation":[{"name":"Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tokyo 321-3497, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryunosuke","family":"Shibata","sequence":"additional","affiliation":[{"name":"Sensory Science Research, Kao Corporation, 2-1-3 Bunka, Sumida-ku, Tokyo 131-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirohiko","family":"Ishida","sequence":"additional","affiliation":[{"name":"Sensory Science Research, Kao Corporation, 2-1-3 Bunka, Sumida-ku, Tokyo 131-8501, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6208-0323","authenticated-orcid":false,"given":"Takuya","family":"Mori","sequence":"additional","affiliation":[{"name":"Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tokyo 321-3497, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0428-7160","authenticated-orcid":false,"given":"Noriyasu","family":"Ota","sequence":"additional","affiliation":[{"name":"Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai-machi, Haga-gun, Tokyo 321-3497, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,23]]},"reference":[{"key":"ref_1","first-page":"e7423","article-title":"Severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2): An update","volume":"12","author":"Pal","year":"2020","journal-title":"Cureus"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1038\/s41579-020-00468-6","article-title":"Coronavirus biology and replication: Implications for SARS-CoV-2","volume":"19","author":"Kratzel","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"eabd9149","DOI":"10.1126\/science.abd9149","article-title":"Airborne transmission of respiratory viruses","volume":"373","author":"Wang","year":"2021","journal-title":"Science"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1016\/0278-6915(89)90016-1","article-title":"Subchronic nose-only inhalation study of propylene glycol in Sprague-Dawley rats","volume":"27","author":"Suber","year":"1989","journal-title":"Food Chem. Toxicol."},{"key":"ref_5","first-page":"38","article-title":"A review on the safety of inhalation of propylene glycol in E-cigarettes","volume":"2","author":"Uddin","year":"2018","journal-title":"World J. Pharm. Pharm. Sci."},{"key":"ref_6","unstructured":"US EPA (2021). Estimation Programs Interface SuiteTM for Microsoft\u00ae."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"148","DOI":"10.2340\/0001555571148150","article-title":"Antibacterial and antifungal properties of propylene glycol, hexylene glycol, and 1,3-butylene glycol in vitro","volume":"71","author":"Kinnunen","year":"1991","journal-title":"Acta Derm. Venereol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"114","DOI":"10.4103\/2231-0762.155736","article-title":"Bactericidal activity of propylene glycol, glycerine, polyethylene glycol 400, and polyethylene glycol 1000 against selected microorganisms","volume":"5","author":"Nalawade","year":"2015","journal-title":"J. Int. Soc. Prev. Community Dent."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1084\/jem.78.5.387","article-title":"The bactericidal action of propylene glycol vapor on microorganisms suspended in air: II. The influence of various factors on the activity of the vapor","volume":"78","author":"Puck","year":"1943","journal-title":"J. Exp. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1126\/science.94.2452.612.b","article-title":"The protection of mice AGAINST infection WITH air-borne influenza virus by means of propylene glycol vapor","volume":"94","author":"Robertson","year":"1941","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1188","DOI":"10.1248\/bpb.32.1188","article-title":"Glucosyl hesperidin prevents influenza A virus replication in vitro by inhibition of viral sialidase","volume":"32","author":"Saha","year":"2009","journal-title":"Biol. Pharm. Bull."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.antiviral.2010.06.008","article-title":"Antiviral effect of strictinin on influenza virus replication","volume":"88","author":"Saha","year":"2010","journal-title":"Antivir. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"103894","DOI":"10.1016\/j.jff.2020.103894","article-title":"Green tea catechins adsorbed on the murine pharyngeal mucosa reduce influenza A virus infection","volume":"68","author":"Onishi","year":"2020","journal-title":"J. Funct. Foods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5940","DOI":"10.1128\/JVI.02496-07","article-title":"Sulfatide is required for efficient replication of influenza A virus","volume":"82","author":"Takahashi","year":"2008","journal-title":"J. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1586\/eri.10.51","article-title":"Viral membranes: An emerging antiviral target for enveloped viruses?","volume":"8","author":"Song","year":"2010","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1049","DOI":"10.1016\/j.chembiol.2010.10.002","article-title":"Positive reinforcement for viruses","volume":"17","author":"Vigant","year":"2010","journal-title":"Chem. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.jct.2005.06.018","article-title":"Measurement and correlation of excess molar enthalpy for (1,2-propanediol, or 1,3-propanediol, or 1,4-butanediol+water) at the temperatures (298.15, 323.15, and 343.15) K","volume":"38","author":"Nagamachi","year":"2006","journal-title":"J. Chem. Thermodyn."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"414","DOI":"10.3358\/shokueishi.31.414","article-title":"Differential scanning colorimetric thermography of water-alcohol mixtures and bactericidal action","volume":"31","author":"Beppu","year":"1990","journal-title":"J. Food Hyg. Soc. Jpn."},{"key":"ref_19","first-page":"168","article-title":"Clusters in whiskey","volume":"47","author":"Nishi","year":"1994","journal-title":"Chem. Ind."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1099\/vir.0.83393-0","article-title":"Protective effect of low-concentration chlorine dioxide gas against influenza A virus infection","volume":"89","author":"Ogata","year":"2008","journal-title":"J. Gen. Virol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.ajic.2020.08.022","article-title":"Effectiveness of 222-nm ultraviolet light on disinfecting SARS-CoV-2 surface contamination","volume":"49","author":"Kitagawa","year":"2021","journal-title":"Am. J. Infect. Control"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"310","DOI":"10.4058\/jsei.31.310","article-title":"Evaluation of the authenticity of practical usefulness of a commercial chemical product that claims the virucidal ability by releasing gas of chlorine dioxide: Investigation on inactivation of Air-borne influenza virus under a room temperature and humidity of the winter season","volume":"31","author":"Nishimura","year":"2016","journal-title":"Jpn. J. Environ. Infect."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Hammond, A., Khalid, T., Thornton, H.V., Woodall, C.A., and Hay, A.D. (2021). Should homes and workplaces purchase portable air filters to reduce the transmission of SARS-CoV-2 and other respiratory infections? A systematic review. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0251049"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1084\/jem.85.6.741","article-title":"The mechanism of aerial disinfection by glycols and other chemical agents: II. An analysis of the factors governing the efficiency of chemical disinfection of the air","volume":"85","author":"Puck","year":"1947","journal-title":"J. Exp. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1084\/jem.85.6.729","article-title":"The mechanism of aerial disinfection by glycols and other chemical agents: I. Demonstration that the germicidal action occurs through the agency of the vapor phase","volume":"85","author":"Puck","year":"1947","journal-title":"J. Exp. Med."},{"key":"ref_26","first-page":"47","article-title":"Anti-influenza virus activity of essential oils and vapors","volume":"2","author":"Vimalanathan","year":"2014","journal-title":"Am. J. Essent. Oils Nat. Prod."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1055\/s-2006-958063","article-title":"Virucidal effects of the steam distillate from Houttuynia cordata and its components on HSV-1, influenza virus, and HIV","volume":"61","author":"Hayashi","year":"1995","journal-title":"Planta Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jaerosci.2013.01.004","article-title":"Antiviral activity of tea tree and eucalyptus oil aerosol and vapour","volume":"59","author":"Usachev","year":"2013","journal-title":"J. Aerosol Sci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"253643","DOI":"10.1093\/ecam\/nep187","article-title":"Screening for antiviral activities of isolated compounds from essential oils","volume":"2011","author":"Astani","year":"2011","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"348","DOI":"10.24171\/j.phrp.2018.9.6.09","article-title":"Chemical constituents of essential oils possessing anti-influenza A\/WS\/33 virus activity","volume":"9","author":"Choi","year":"2018","journal-title":"Osong Public Health Res. Perspect."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1177\/0960327113506722","article-title":"Octanal-induced inflammatory responses in cells relevant for lung toxicity: Expression and release of cytokines in A549 human alveolar cells","volume":"33","author":"Song","year":"2014","journal-title":"Human Exp. Toxicol."},{"key":"ref_32","first-page":"52","article-title":"Tests for the chronic toxicity of propylene glycol and triethylene glycol on monkeys and rats by vapor inhalation and oral administration","volume":"91","author":"Robertson","year":"1947","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"ref_33","unstructured":"(2023, May 08). Toxicological Summary of Propane-1,2-diol. Available online: https:\/\/echa.europa.eu\/registration-dossier\/-\/registered-dossier\/16001\/7\/1."}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/7\/1421\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:59:08Z","timestamp":1760126348000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/7\/1421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,23]]},"references-count":33,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["v15071421"],"URL":"https:\/\/doi.org\/10.3390\/v15071421","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,23]]}}}