{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:18:16Z","timestamp":1760149096144,"version":"build-2065373602"},"reference-count":56,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,27]],"date-time":"2023-06-27T00:00:00Z","timestamp":1687824000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICB\/USP Graduate Program","award":["2021\/05661-1","2020\/08943-5"],"award-info":[{"award-number":["2021\/05661-1","2020\/08943-5"]}]},{"name":"RAS","award":["2021\/05661-1","2020\/08943-5"],"award-info":[{"award-number":["2021\/05661-1","2020\/08943-5"]}]},{"name":"LMRJ","award":["2021\/05661-1","2020\/08943-5"],"award-info":[{"award-number":["2021\/05661-1","2020\/08943-5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The Zika virus (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described as sexually and vertically transmitted. The latter shows severe consequences for the embryo\/fetus, such as congenital microcephaly and deficiency of the neural system, currently known as Congenital ZIKV Syndrome (CZS). To better understand ZIKV dynamics in trophoblastic cells present in the first trimester of pregnancy (BeWo, HTR-8, and control cell HuH-7), an experiment of viral kinetics was performed for African MR766 low passage and Asian-Brazilian IEC ZIKV lineages. The results were described independently and demonstrated that the three placental cells lines are permissive and susceptible to ZIKV. We noticed cytopathic effects that are typical in in vitro viral infection in BeWo and HTR-8. Regarding kinetics, MR766lp showed peaks of viral loads in 24 and 48 hpi for all cell types tested, as well as marked cells death after peak production. On the other hand, the HTR-8 lineage inoculated with ZIKV-IEC exhibited increased viral production in 144 hpi, with a peak between 24 and 96 hpi. Furthermore, IEC had peak variations of viral production for BeWo in 144 hpi. Considering such in vitro results, the hypothesis that maternal fetal transmission is probably a way of virus transmission between the mother and the embryo\/fetus is maintained.<\/jats:p>","DOI":"10.3390\/v15071446","type":"journal-article","created":{"date-parts":[[2023,6,28]],"date-time":"2023-06-28T00:25:21Z","timestamp":1687911921000},"page":"1446","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Proposal of Model for Evaluation of Viral Kinetics of African\/Asian\/Brazilian\u2014Zika virus Strains (Step Growth Curve) in Trophoblastic Cell Lines"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-8013-7508","authenticated-orcid":false,"given":"M\u00e1rcia Duarte","family":"Barbosa","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Institute of Biosciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Laboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04039-032, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anderson","family":"Costa","sequence":"additional","affiliation":[{"name":"Laboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04039-032, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Prieto-Oliveira","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics and Genomics, College of Computing and Informatics, University of North Carolina at Charlotte, 9331 Robert D. Snyder Rd, Charlotte, NC 28223, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Andreata-Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04039-032, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina M.","family":"Peter","sequence":"additional","affiliation":[{"name":"Laboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04039-032, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8167-0625","authenticated-orcid":false,"given":"Paolo M. A.","family":"Zanotto","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Evolution and Bioinformatics, Department of Microbiology, Institute of Biosciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Mario Ramos","family":"Janini","sequence":"additional","affiliation":[{"name":"Laboratory of Retrovirology, Department of Microbiology, Immunology and Parasitology, Discipline of Microbiology, Federal University of S\u00e3o Paulo, S\u00e3o Paulo 04039-032, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/0035-9203(52)90042-4","article-title":"Zika virus (I). Isolations and serological specificity","volume":"46","author":"Dick","year":"1952","journal-title":"Trans. R. Soc. Trop. Med. 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