{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:37:57Z","timestamp":1788907077702,"version":"build-2803163510"},"reference-count":97,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:00:00Z","timestamp":1688083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Application of bacteriophages against highly pathogenic bacteria in model studies","award":["STCU P486"],"award-info":[{"award-number":["STCU P486"]}]},{"name":"Directorate General for International Relations and Strategy (DGRIS)","award":["STCU P486"],"award-info":[{"award-number":["STCU P486"]}]},{"name":"French Agency for International Technical Expertise (AFETI)","award":["STCU P486"],"award-info":[{"award-number":["STCU P486"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Phages of highly pathogenic bacteria represent an area of growing interest for bacterial detection and identification and subspecies typing, as well as for phage therapy and environmental decontamination. Eight new phages\u2014YpEc56, YpEc56D, YpEc57, YpEe58, YpEc1, YpEc2, YpEc11, and YpYeO9\u2014expressing lytic activity towards Yersinia pestis revealed a virion morphology consistent with the Podoviridae morphotype. These phages lyse all 68 strains from 2 different sets of Y. pestis isolates, thus limiting their potential application for subtyping of Y. pestis strains but making them rather promising in terms of infection control. Two phages\u2014YpYeO9 and YpEc11\u2014were selected for detailed studies based on their source of isolation and lytic cross activity towards other Enterobacteriaceae. The full genome sequencing demonstrated the virulent nature of new phages. Phage YpYeO9 was identified as a member of the Teseptimavirus genus and YpEc11 was identified as a member of the Helsettvirus genus, thereby representing new species. A bacterial challenge assay in liquid microcosm with a YpYeO9\/YpEc11 phage mixture showed elimination of Y. pestis EV76 during 4 h at a P\/B ratio of 1000:1. These results, in combination with high lysis stability results of phages in liquid culture, the low frequency of formation of phage resistant mutants, and their viability under different physical\u2013chemical factors indicate their potential for their practical use as an antibacterial mean.<\/jats:p>","DOI":"10.3390\/v15071484","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T16:08:32Z","timestamp":1688141312000},"page":"1484","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["New Bacteriophages with Podoviridal Morphotypes Active against Yersinia pestis: Characterization and Application Potential"],"prefix":"10.3390","volume":"15","author":[{"given":"Tamar","family":"Suladze","sequence":"first","affiliation":[{"name":"George Eliava Institute of Bacteriophages, Microbiology and Virology (Eliava IBMV), 3, Gotua Str., 0160 Tbilisi, 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