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Chimeric viruses were constructed based on an infectious cDNA clone derived from the live attenuated GPE\u2212 vaccine strain as novel CSF vaccine candidates that potentially meet the concept of differentiating infected from vaccinated animals (DIVA) by substituting the glycoprotein Erns of the GPE\u2212 vaccine strain with the corresponding region of non-CSF pestiviruses, either pronghorn antelope pestivirus (PAPeV) or Phocoena pestivirus (PhoPeV). High viral growth and genetic stability after serial passages of the chimeric viruses, namely vGPE\u2212\/PAPeV Erns and vGPE\u2212\/PhoPeV Erns, were confirmed in vitro. In vivo investigation revealed that two chimeric viruses had comparable immunogenicity and safety profiles to the vGPE\u2212 vaccine strain. Vaccination at a dose of 104.0 TCID50 with either vGPE\u2212\/PAPeV Erns or vGPE\u2212\/PhoPeV Erns conferred complete protection for pigs against the CSF virus challenge in the early stage of immunization. In conclusion, the characteristics of vGPE\u2212\/PAPeV Erns and vGPE\u2212\/PhoPeV Erns affirmed their properties, as the vGPE\u2212 vaccine strain, positioning them as ideal candidates for future development of a CSF marker vaccine.<\/jats:p>","DOI":"10.3390\/v15071587","type":"journal-article","created":{"date-parts":[[2023,7,21]],"date-time":"2023-07-21T01:52:35Z","timestamp":1689904355000},"page":"1587","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Generation and Efficacy of Two Chimeric Viruses Derived from GPE\u2212 Vaccine Strain as Classical Swine Fever Vaccine Candidates"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9922-4802","authenticated-orcid":false,"given":"Loc Tan","family":"Huynh","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan"},{"name":"Faculty of Veterinary Medicine, College of Agriculture, Can Tho University, Can Tho 900000, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3480-988X","authenticated-orcid":false,"given":"Norikazu","family":"Isoda","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan"},{"name":"One Health Research Center, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan"},{"name":"International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Hokkaido, Japan"},{"name":"Hokkaido University Institute for Vaccine Research and Development (HU-IVReD), Hokkaido University, Sapporo 001-0021, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lim Yik","family":"Hew","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saho","family":"Ogino","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yume","family":"Mimura","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Hokkaido, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maya","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary 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