{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:23:21Z","timestamp":1760149401143,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,29]],"date-time":"2023-07-29T00:00:00Z","timestamp":1690588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Key Industrial Innovation Chain (Group) in Agriculture, Shaanxi, China","award":["2022ZDLNY01-10"],"award-info":[{"award-number":["2022ZDLNY01-10"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Adenovirus vectors possess a good safety profile, an extensive genome, a range of host cells, high viral yield, and the ability to elicit broad humoral and cellular immune responses. Adenovirus vectors are widely used in infectious disease research for future vaccine development and gene therapy. In this study, we obtained a fowl adenovirus serotype 4 (FAdV-4) isolate from sick chickens with hepatitis\u2013hydropericardium syndrome (HHS) and conducted animal regression text to clarify biological pathology. We amplified the transfer vector and extracted viral genomic DNA from infected LMH cells, then recombined the mixtures via the Gibson assembly method in vitro and electroporated them into EZ10 competent cells to construct the FAdV-4 infectious clone. The infectious clones were successfully rescued in LMH cells within 15 days of transfection. The typical cytopathic effect (CPE) and propagation titer of FAdV-4 infectious clones were also similar to those for wild-type FAdV-4. To further construct the single-cycle adenovirus (SC-Ad) vector, we constructed SC-Ad vectors by deleting the gene for IIIa capsid cement protein. The FAdV4 infectious clone vector was introduced into the ccdB cm expression cassette to replace the IIIa gene using a \u03bb-red homologous recombination technique, and then the ccdB cm expression cassette was excised by PmeI digestion and self-ligation to obtain the resulting plasmids as SC-Ad vectors.<\/jats:p>","DOI":"10.3390\/v15081657","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T01:28:46Z","timestamp":1690766926000},"page":"1657","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rapid Construction of an Infectious Clone of Fowl Adenovirus Serotype 4 Isolate"],"prefix":"10.3390","volume":"15","author":[{"given":"Minzhi","family":"Gong","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yating","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shijia","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boshuo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enqi","family":"Du","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China"},{"name":"Yangling Carey Biotechnology Co., Ltd., Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yupeng","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1159\/000149541","article-title":"Grouping of fowl adenoviruses based upon the restriction patterns of DNA generated by BamHI and HindIII","volume":"22","author":"Kisary","year":"1984","journal-title":"Intervirol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1128\/JCM.01567-08","article-title":"Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region","volume":"47","author":"Steer","year":"2009","journal-title":"J. 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