{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T08:01:05Z","timestamp":1782633665011,"version":"3.54.5"},"reference-count":29,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"ICMR-NIV institutional","award":["ENV-1701"],"award-info":[{"award-number":["ENV-1701"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Astroviruses (AstV) and adenoviruses (AdV) are associated with diarrhoea in young animals. However, the epidemiology and genetic diversity of AstVs and AdVs in animals is not well studied. Hence, the present study was conducted to detect and characterize AstVs and AdVs in calves, piglets and puppies from Western Maharashtra, India. Out of the processed porcine (48), canine (80), and bovine (65) faecal samples, the porcine AstV (PAstV), bovine AstV (BAstV), canine AstV (CAstV), and porcine AdV (PAdV) were detected in 12.5%, 7.69%, 3.75% and 4.1% of samples, respectively. In the RNA-dependent RNA polymerase region-based phylogenetic analysis, the detected BAstV strains grouped with MAstV-28, MAstV-33, and MAstV-35, CAstV strains belonged to MAstV-5; PAstV strains belonged to MAstV-24, MAstV-26, and MAstV-31. However, in hexon gene-based phylogeny, both the detected PAdV were of genotype 3, exhibiting 91.9\u201392.5% nucleotide identity with Ivoirian and Chinese strains. The study reports first-time BAstVs from calves and PAdV-3 from piglets in India. The study revealed diversity in the circulation of AstVs in tested animals and AdVs in pigs, and suggested that they alone might be associated with other diarrhoea or in combination with other enteric pathogens, thus highlighting the necessity of extensive epidemiological investigations to develop diagnostic tools and control measures.<\/jats:p>","DOI":"10.3390\/v15081679","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:14:40Z","timestamp":1690881280000},"page":"1679","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection and Molecular Characterization of Animal Adenovirus and Astrovirus from Western Maharashtra, India"],"prefix":"10.3390","volume":"15","author":[{"given":"Pradeep M.","family":"Sawant","sequence":"first","affiliation":[{"name":"Enteric Viruses Group, ICMR\u2014National Institute of Virology, 20-A, Ambedkar Road, Pune 411 001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rishabh B.","family":"Waghchaure","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, ICMR\u2014National Institute of Virology, 20-A, Ambedkar Road, Pune 411 001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja A.","family":"Shinde","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, ICMR\u2014National Institute of Virology, 20-A, Ambedkar Road, Pune 411 001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avani P.","family":"Palikondawar","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, ICMR\u2014National Institute of Virology, 20-A, Ambedkar Road, Pune 411 001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mallika","family":"Lavania","sequence":"additional","affiliation":[{"name":"Enteric Viruses Group, ICMR\u2014National Institute of Virology, 20-A, Ambedkar Road, Pune 411 001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.3390\/microbiolres12030048","article-title":"Viral Enteritis in Cattle: To Well Known Viruses and Beyond","volume":"12","author":"Castells","year":"2021","journal-title":"Microbiol. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"974","DOI":"10.1111\/tbed.14123","article-title":"Scoping review on the epidemiology, diagnostics and clinical significance of porcine astroviruses","volume":"69","author":"Rawal","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Malik, Y.S., Singh, R.K., and Yadav, M.P. (2019). Recent Advances in Animal Virology, Springer.","DOI":"10.1007\/978-981-13-9073-9"},{"key":"ref_4","first-page":"001721","article-title":"ICTV Report Consortium. ICTV Virus Taxonomy Profile: Adenoviridae 2022","volume":"103","author":"Aoki","year":"2022","journal-title":"J. Gen. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.meegid.2018.10.026","article-title":"Molecular epidemiology and characterization of porcine adenoviruses in pigs with diarrhoea in Thailand","volume":"67","author":"Kumthip","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Zhu, Q., Li, B., and Sun, D. (2022). Bovine Astrovirus-A Comprehensive Review. Viruses, 14.","DOI":"10.3390\/v14061217"},{"key":"ref_7","unstructured":"Bosch, A., King, A.M., Lefkowitz, E., Adams, M.J., and Carstens, E.B. (2011). Family Astroviridae. Virus Taxonomy: Classification and Nomenclature of Viruses (Ninth Report of the International Committee on the Taxonomy of Viruses), Academic Press. [9th ed.]."},{"key":"ref_8","unstructured":"(2023, July 01). 20th Livestock Census, Available online: https:\/\/pib.gov.in\/PressReleaseIframePage.aspx?PRID=1813802."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"6596","DOI":"10.1128\/JVI.76.13.6596-6601.2002","article-title":"Molecular characterization of VP6 genes of human rotavirus isolates: Correlation of genogroups with subgroups and evidence of independent segregation","volume":"76","author":"Wong","year":"2002","journal-title":"J. Virol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"104385","DOI":"10.1016\/j.meegid.2020.104385","article-title":"Molecular characterization of unusual G10P[33], G6P[14] genomic constellations of group A rotavirus and evidence of zooanthroponosis in bovines","volume":"84","author":"Sawant","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5001","DOI":"10.1128\/AEM.00625-11","article-title":"Detection of Known and Novel Adenoviruses in Cattle Wastes via Broad-Spectrum Primers","volume":"77","author":"Sibley","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"9107","DOI":"10.1128\/JVI.00857-08","article-title":"Novel Astroviruses in Insectivorous Bats","volume":"82","author":"Chu","year":"2008","journal-title":"J. Virol."},{"key":"ref_13","first-page":"001640","article-title":"Prevalence of bovine astroviruses and their genotypes in sampled Chinese calves with and without diarrhoea","volume":"2","author":"Zhu","year":"2021","journal-title":"J. Gen. Virol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.meegid.2017.02.008","article-title":"Whole genome analysis of porcine astroviruses detected in Japanese pigs reveals genetic diversity and possible intra-genotypic recombination","volume":"50","author":"Ito","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1186\/s12985-017-0823-4","article-title":"Detection and genetic characterization of canine astroviruses in pet dogs in Guangxi, China","volume":"14","author":"Zhou","year":"2017","journal-title":"Virol. J."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gainor, K., Fortuna, Y.C., Alakkaparambil, A.S., Gonz\u00e1lez, W., Malik, Y.S., and Ghosh, S. (2022). High Rates of Detection and Molecular Characterization of Porcine Adenovirus Serotype 5 (Porcine mastadenovirus C) from Diarrheic Pigs. Pathogens, 11.","DOI":"10.3390\/pathogens11101210"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Vu, D.-L., Bosch, A., Pint\u00f3, R.M., and Guix, S. (2017). Epidemiology of Classic and Novel Human Astrovirus: Gastroenteritis and Beyond. Viruses, 9.","DOI":"10.3390\/v9020033"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1679","DOI":"10.1080\/22221751.2019.1690953","article-title":"Are adenoviruses zoonotic? A systematic review of the evidence","volume":"8","author":"Borkenhagen","year":"2019","journal-title":"Emerg. Microbes Infect."},{"key":"ref_19","unstructured":"Curiel, D.T. (2016). Adenoviral Vectors for Gene Therapy, Academic Press. [2nd ed.]."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1016\/j.meegid.2011.07.024","article-title":"Astrovirus infections in humans and animals\u2014Molecular biology, genetic diversity, and interspecies transmissions","volume":"11","author":"Burnham","year":"2011","journal-title":"Infect. Genet. Evol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1007\/s00705-018-3806-x","article-title":"Detection and genetic characterization of porcine astroviruses in piglets with and without diarrhoea in Thailand","volume":"163","author":"Kumthip","year":"2018","journal-title":"Arch. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3079","DOI":"10.1007\/s00705-015-2569-x","article-title":"Identification and genetic characterization of two porcine astroviruses from domestic piglets in China","volume":"160","author":"Li","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1111\/tbed.13058","article-title":"First report and genetic characterization of porcine astroviruses of lineage 4 and 2 in diarrhoeic pigs in India","volume":"66","author":"Kattoor","year":"2019","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2847","DOI":"10.1007\/s00705-021-05195-8","article-title":"Molecular detection and characterization reveals circulation of multiple genotypes of porcine astrovirus in Haryana, India","volume":"166","author":"Kour","year":"2021","journal-title":"Arch. Virol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2491","DOI":"10.1007\/s00705-015-2543-7","article-title":"Full genome analysis of bovine astrovirus from fecal samples of cattle in Japan: Identification of possible interspecies transmission of bovine astrovirus","volume":"160","author":"Nagai","year":"2015","journal-title":"Arch. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1007\/s00705-022-05674-6","article-title":"Comprehensive molecular survey of viral pathogens associated with canine gastroenteritis","volume":"168","author":"Dema","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1007\/s11262-013-1013-0","article-title":"Phylogenetic analysis of bovine astrovirus in Korean cattle","volume":"48","author":"Oem","year":"2014","journal-title":"Virus Genes"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.vetmic.2015.05.002","article-title":"High prevalence and diversity of bovine astroviruses in the faeces of healthy and diarrhoeic calves in South West Scotland","volume":"178","author":"Sharp","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1002\/jmv.25645","article-title":"Epidemiology and clinical features of rotavirus, adenovirus, and astrovirus infections and coinfections in children with acute gastroenteritis prior to rotavirus vaccine introduction in Meerut, North India","volume":"92","author":"Akdag","year":"2020","journal-title":"J. Med. 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