{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:08:27Z","timestamp":1788379707721,"version":"build-2803163510"},"reference-count":38,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016512","name":"Center for Companion Animal Health","doi-asserted-by":"publisher","award":["2021-61-F"],"award-info":[{"award-number":["2021-61-F"]}],"id":[{"id":"10.13039\/100016512","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Nucleoside analogs GS-441524 and remdesivir (GS-5734) are effective in treating cats with feline infectious peritonitis (FIP). However, no studies have compared the efficacy between antiviral medications. The objective of this study was to evaluate the efficacy of orally administered GS-442514 (12.5\u201315 mg\/kg) compared to orally administered remdesivir (25\u201330 mg\/kg) in a double-blinded non-inferiority trial. Eighteen cats with effusive FIP were prospectively enrolled and randomly assigned to receive either GS-442514 or remdesivir. Cats were treated daily for 12 weeks and evaluated at week 0, 12, and 16. Survival and disease remission at week 16 were compared between groups. Five of 9 (55%) cats treated GS-441524 and 7\/9 (77%) cats treated with remdesivir survived, with no difference in survival rate (p = 0.2). Remdesivir fulfilled the criteria for non-inferiority with a difference in survival of 22% (90% CI; \u221213.5\u201357.5%). Three of the 18 cats died within 48 h of enrollment. Excluding these cats, 5\/6 (83%) of the cats treated with GS-441524 and 7\/9 (77%) of the cats treated with remdesivir survived. These findings suggest that both orally administered GS-441524 and remdesivir are safe and effective anti-viral medications for the treatment of effusive FIP. Further optimization of the first 48 h of treatment is needed.<\/jats:p>","DOI":"10.3390\/v15081680","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T09:14:40Z","timestamp":1690881280000},"page":"1680","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Efficacy of Oral Remdesivir Compared to GS-441524 for Treatment of Cats with Naturally Occurring Effusive Feline Infectious Peritonitis: A Blinded, Non-Inferiority Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Emma","family":"Cosaro","sequence":"first","affiliation":[{"name":"William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jully","family":"Pires","sequence":"additional","affiliation":[{"name":"Veterinary Center for Clinical Trials, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Castillo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0057-0604","authenticated-orcid":false,"given":"Brian G.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3426-6352","authenticated-orcid":false,"given":"Krystle L.","family":"Reagan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1006\/viro.1998.9045","article-title":"Feline infectious peritonitis viruses arise by mutation from endemic feline enteric coronaviruses","volume":"243","author":"Vennema","year":"1998","journal-title":"Virology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/j.cvsm.2011.08.004","article-title":"Feline coronavirus in multicat environments","volume":"41","author":"Drechsler","year":"2011","journal-title":"Vet. Clin. Small Anim. Pract."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.tvjl.2014.04.017","article-title":"An update on feline infectious peritonitis: Virology and immunopathogenesis","volume":"201","author":"Pedersen","year":"2014","journal-title":"Vet. J."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jfms.2008.09.008","article-title":"A review of feline infectious peritonitis virus infection: 1963\u20132008","volume":"11","author":"Pedersen","year":"2009","journal-title":"J. Feline Med. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.vetmic.2018.04.026","article-title":"The nucleoside analog GS-441524 strongly inhibits feline infectious peritonitis (FIP) virus in tissue culture and experimental cat infection studies","volume":"219","author":"Murphy","year":"2018","journal-title":"Vet. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1177\/1098612X19825701","article-title":"Efficacy and safety of the nucleoside analog GS-441524 for treatment of cats with naturally occurring feline infectious peritonitis","volume":"21","author":"Pedersen","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Jones, S., Novicoff, W., Nadeau, J., and Evans, S. (2021). Unlicensed GS-441524-like antiviral therapy can be effective for at-home treatment of feline infectious peritonitis. Animals, 11.","DOI":"10.3390\/ani11082257"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1587","DOI":"10.1111\/jvim.15780","article-title":"Antiviral treatment using the adenosine nucleoside analogue GS-441524 in cats with clinically diagnosed neurological feline infectious peritonitis","volume":"34","author":"Dickinson","year":"2020","journal-title":"J. Vet. Intern. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Krentz, D., Zenger, K., Alberer, M., Felten, S., Bergmann, M., Dorsch, R., Matiasek, K., Kolberg, L., Hofmann-Lehmann, R., and Meli, M.L. (2021). Curing cats with feline infectious peritonitis with an oral multi-component drug containing GS-441524. Viruses, 13.","DOI":"10.3390\/v13112228"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Katayama, M., and Uemura, Y. (2023). Prognostic Prediction for Therapeutic Effects of Mutian on 324 Client-Owned Cats with Feline Infectious Peritonitis Based on Clinical Laboratory Indicators and Physical Signs. Vet. Sci., 10.","DOI":"10.3390\/vetsci10020136"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Green, J., Syme, H., and Tayler, S. (2023). Thirty-two cats with effusive or non-effusive feline infectious peritonitis treated with a combination of remdesivir and GS-441524. J. Vet. Intern. Med.","DOI":"10.1111\/jvim.16804"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Coggins, S.J., Norris, J.M., Malik, R., Govendir, M., Hall, E.J., Kimble, B., and Thompson, M.F. (2023). Outcomes of treatment of cats with feline infectious peritonitis using parenterally administered remdesivir, with or without transition to orally administered GS-441524. J. Vet. Intern. Med.","DOI":"10.1111\/jvim.16803"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"105933","DOI":"10.1016\/j.ijantimicag.2020.105933","article-title":"Arguments in favour of remdesivir for treating SARS-CoV-2 infections","volume":"55","author":"Ko","year":"2020","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2785","DOI":"10.1021\/acs.jmedchem.0c01929","article-title":"Remdesivir metabolite GS-441524 effectively inhibits SARS-CoV-2 infection in mouse models","volume":"65","author":"Li","year":"2021","journal-title":"J. Med. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"100128","DOI":"10.1016\/j.onehlt.2020.100128","article-title":"Current knowledge about the antivirals remdesivir (GS-5734) and GS-441524 as therapeutic options for coronaviruses","volume":"9","author":"Amirian","year":"2020","journal-title":"One Health"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Cook, S., Wittenburg, L., Yan, V.C., Theil, J.H., Castillo, D., Reagan, K.L., Williams, S., Pham, C.-D., Li, C., and Muller, F.L. (2022). An Optimized Bioassay for Screening Combined Anticoronaviral Compounds for Efficacy against Feline Infectious Peritonitis Virus with Pharmacokinetic Analyses of GS-441524, Remdesivir, and Molnupiravir in Cats. Viruses, 14.","DOI":"10.3390\/v14112429"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Felten, S., and Hartmann, K. (2019). Diagnosis of feline infectious peritonitis: A review of the current literature. Viruses, 11.","DOI":"10.3390\/v11111068"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Stranieri, A., Scavone, D., Paltrinieri, S., Giordano, A., Bonsembiante, F., Ferro, S., Gelain, M.E., Meazzi, S., and Lauzi, S. (2020). Concordance between histology, immunohistochemistry, and RT-PCR in the diagnosis of feline infectious peritonitis. Pathogens, 9.","DOI":"10.3390\/pathogens9100852"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1177\/1098612X16630357","article-title":"Investigation into the utility of an immunocytochemical assay in body cavity effusions for diagnosis of feline infectious peritonitis","volume":"19","author":"Felten","year":"2016","journal-title":"J. Feline Med. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1748-5827.1993.tb02591.x","article-title":"Using direct immunofluorescence to detect coronaviruses in peritoneal in peritoneal and pleural effusions","volume":"34","author":"Parodi","year":"1993","journal-title":"J. Small Anim. Pract."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.tvjl.2013.08.023","article-title":"Diagnostic utility of a direct immunofluorescence test to detect feline coronavirus antigen in macrophages in effusive feline infectious peritonitis","volume":"198","author":"Litster","year":"2013","journal-title":"Vet. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1177\/1098612X18809165","article-title":"Diagnosis of non-effusive feline infectious peritonitis by reverse transcriptase quantitative PCR from mesenteric lymph node fine-needle aspirates","volume":"21","author":"Dunbar","year":"2019","journal-title":"J. Feline Med. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1177\/1098612X19886671","article-title":"Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis","volume":"22","author":"Emmler","year":"2020","journal-title":"J. Feline Med. Surg."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12917-017-1147-8","article-title":"Sensitivity and specificity of a real-time reverse transcriptase polymerase chain reaction detecting feline coronavirus mutations in effusion and serum\/plasma of cats to diagnose feline infectious peritonitis","volume":"13","author":"Felten","year":"2017","journal-title":"BMC Vet. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1128\/jcm.33.3.684-689.1995","article-title":"Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR","volume":"33","author":"Herrewegh","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0378-1135(98)00210-7","article-title":"Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis","volume":"62","author":"Harbour","year":"1998","journal-title":"Vet. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1177\/1098612X15586209","article-title":"Clinical and laboratory features of cats with feline infectious peritonitis\u2013a retrospective study of 231 confirmed cases (2000\u20132010)","volume":"18","author":"Riemer","year":"2016","journal-title":"J. Feline Med. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jfms.2010.09.014","article-title":"Clinicopathological findings and disease staging of feline infectious peritonitis: 51 cases from 2003 to 2009 in Taiwan","volume":"13","author":"Tsai","year":"2011","journal-title":"J. Feline Med. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1111\/j.1939-1676.2003.tb02515.x","article-title":"Comparison of different tests to diagnose feline infectious peritonitis","volume":"17","author":"Hartmann","year":"2003","journal-title":"J. Vet. Intern. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1177\/1098612X12454862","article-title":"Positive predictive value of albumin: Globulin ratio for feline infectious peritonitis in a mid-western referral hospital population","volume":"14","author":"Jeffery","year":"2012","journal-title":"J. Feline Med. Surg."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/j.jfms.2008.02.006","article-title":"Pathogenesis of feline enteric coronavirus infection","volume":"10","author":"Pedersen","year":"2008","journal-title":"J. Feline Med. Surg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.jcrc.2005.04.005","article-title":"Randomization and allocation concealment: A practical guide for researchers","volume":"20","author":"Doig","year":"2005","journal-title":"J. Crit. Care"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1111\/j.1476-4431.2008.00312.x","article-title":"Evaluation of a thoracic focused assessment with sonography for trauma (TFAST) protocol to detect pneumothorax and concurrent thoracic injury in 145 traumatized dogs","volume":"18","author":"Lisciandro","year":"2008","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1111\/j.1476-4431.2009.00459.x","article-title":"Evaluation of an abdominal fluid scoring system determined using abdominal focused assessment with sonography for trauma in 101 dogs with motor vehicle trauma","volume":"19","author":"Lisciandro","year":"2009","journal-title":"J. Vet. Emerg. Crit. Care"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1007\/s11606-010-1513-8","article-title":"Understanding equivalence and noninferiority testing","volume":"26","author":"Walker","year":"2011","journal-title":"J. Gen. Intern. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.vetmic.2014.10.025","article-title":"Levels of feline infectious peritonitis virus in blood, effusions, and various tissues and the role of lymphopenia in disease outcome following experimental infection","volume":"175","author":"Pedersen","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1016\/j.dsx.2020.05.018","article-title":"Remdesivir in COVID-19: A critical review of pharmacology, pre-clinical and clinical studies","volume":"14","author":"Singh","year":"2020","journal-title":"Diabetes Metab. Syndr. Clin. Res. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.reprotox.2022.05.012","article-title":"Remdesivir impairs mouse preimplantation embryo development at therapeutic concentrations","volume":"111","author":"Marikawa","year":"2022","journal-title":"Reprod. 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