{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T11:03:17Z","timestamp":1761562997625,"version":"build-2065373602"},"reference-count":23,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T00:00:00Z","timestamp":1692748800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health USA","award":["U01 AI151698","CRP\/BRA20-03","CRP\/20\/03"],"award-info":[{"award-number":["U01 AI151698","CRP\/BRA20-03","CRP\/20\/03"]}]},{"name":"CRP-ICGEB RESEARCH","award":["U01 AI151698","CRP\/BRA20-03","CRP\/20\/03"],"award-info":[{"award-number":["U01 AI151698","CRP\/BRA20-03","CRP\/20\/03"]}]},{"name":"PON \u201cRicerca e Innovazione\u201d","award":["U01 AI151698","CRP\/BRA20-03","CRP\/20\/03"],"award-info":[{"award-number":["U01 AI151698","CRP\/BRA20-03","CRP\/20\/03"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Since its discovery in early 1916, dengue fever, a common vector-borne illness in Brazil, has resulted in extensive urban outbreaks and poses a serious threat to the public\u2019s health. Understanding the dynamics of Dengue Virus (DENV) serotypes circulating in different regions of Brazil is essential for implementing effective disease control and prevention measures. In response to this urgent need, we conducted an on-site training program in genomic surveillance in collaboration with the Central Laboratory of Health and the Secretary of Health of the Mato Grosso do Sul state. This initiative resulted in the generation of 177 DENV genome sequences collected between May 2021 and May 2022, a period during which over 11,391 dengue fever cases were reported in the state. Through this approach, we were able to identify the co-circulation of two different dengue serotypes (DENV1 and DENV2) as well as the existence of diverse viral lineages within each genotype, suggesting that multiple introduction events of different viral strains occurred in the region. By integrating epidemiological data, our findings unveiled temporal fluctuations in the relative abundance of different serotypes throughout various epidemic seasons, highlighting the complex and changing dynamics of DENV transmission throughout time. These findings demonstrate the value of ongoing surveillance activities in tracking viral transmission patterns, monitoring viral evolution, and informing public health actions.<\/jats:p>","DOI":"10.3390\/v15091790","type":"journal-article","created":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T10:09:53Z","timestamp":1692871793000},"page":"1790","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dengue Fever Surveillance in Mato Grosso do Sul: Insights from Genomic Analysis and Implications for Public Health Strategies"],"prefix":"10.3390","volume":"15","author":[{"given":"Larissa Domingues","family":"Castilho de Arruda","sequence":"first","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"},{"name":"Universidade Federal do Estado do Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5849-7326","authenticated-orcid":false,"given":"Marta","family":"Giovanetti","sequence":"additional","affiliation":[{"name":"Instituto Rene Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil"},{"name":"Sciences and Technologies for Sustainable Development and One Health, Universit\u00e0 Campus Bio-Medico di, 00128 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5521-6448","authenticated-orcid":false,"given":"Vagner","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Coordena\u00e7\u00e3o de Vigil\u00e2ncia, Prepara\u00e7\u00e3o e Resposta \u00e0 Emerg\u00eancias e Desastres (PHE), Organiza\u00e7\u00e3o Pan-Americana da Sa\u00fade\/Organiza\u00e7\u00e3o Mundial da Sa\u00fade (OPAS\/OMS), Bras\u00edlia 70312-970, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Castilhos Souza Umaki","family":"Zardin","sequence":"additional","affiliation":[{"name":"SES-MS-Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gislene Garcia de Castro","family":"Lichs","sequence":"additional","affiliation":[{"name":"SES-MS-Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Asato","sequence":"additional","affiliation":[{"name":"SES-MS-Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Olivia Pascoto","family":"Esposito","sequence":"additional","affiliation":[{"name":"SES-MS-Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miriam","family":"Tokeshi M\u00fcller","sequence":"additional","affiliation":[{"name":"SES-MS-Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3916-2018","authenticated-orcid":false,"given":"Joilson","family":"Xavier","sequence":"additional","affiliation":[{"name":"Instituto Rene Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1997-5786","authenticated-orcid":false,"given":"Hegger","family":"Fritsch","sequence":"additional","affiliation":[{"name":"Instituto Rene Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0830-3909","authenticated-orcid":false,"given":"Mauricio","family":"Lima","sequence":"additional","affiliation":[{"name":"Instituto Rene Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0829-036X","authenticated-orcid":false,"given":"Carla","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"lnstituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine Vieira","family":"Santos","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Hemocentro de Ribeir\u00e3o Preto, Ribeir\u00e3o Preto 14051-140, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livia de Mello Almeida","family":"Maziero","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"},{"name":"Universidade Federal do Estado do Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8621-3338","authenticated-orcid":false,"given":"Danila Fernanda Rodrigues","family":"Frias","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Ahad das Neves","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliane","family":"Ferreira da Silva","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"},{"name":"Universidade Federal do Estado do Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen Caroline","family":"Rodrigues Barretos","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Eduardo","family":"Tsuha Oshiro","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Modafari Goday","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, 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C. de","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Organiza\u00e7\u00e3o Pan-Americana da Sa\u00fade, Organiza\u00e7\u00e3o Mundial da Sa\u00fade, Bras\u00edlia 70312-970, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Fabiano do Carmo","family":"Said","sequence":"additional","affiliation":[{"name":"Organiza\u00e7\u00e3o Pan-Americana da Sa\u00fade, Organiza\u00e7\u00e3o Mundial da Sa\u00fade, Bras\u00edlia 70312-970, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Rosewell","sequence":"additional","affiliation":[{"name":"Organiza\u00e7\u00e3o Pan-Americana da Sa\u00fade, Organiza\u00e7\u00e3o Mundial da Sa\u00fade, Bras\u00edlia 70312-970, DF, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8693-7897","authenticated-orcid":false,"given":"Luiz Henrique Ferraz","family":"Demarchi","sequence":"additional","affiliation":[{"name":"SES-MS-Laborat\u00f3rio Central de Sa\u00fade P\u00fablica de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Croda","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Universidade Federal de Mato Grosso do Sul-UFMS, Campo Grande 79000-000, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6769-9931","authenticated-orcid":false,"given":"Luiz Carlos Junior","family":"Alcantara","sequence":"additional","affiliation":[{"name":"Instituto Rene Rachou, Funda\u00e7\u00e3o Oswaldo Cruz, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6953-4965","authenticated-orcid":false,"given":"Crhistinne","family":"Cavalheiro Maymone Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Secretaria de Estado de Sa\u00fade de Mato Grosso do Sul, Campo Grande 79031-350, MS, Brazil"},{"name":"Universidade Federal do Estado do Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.1038\/s41467-021-22607-0","article-title":"Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil","volume":"12","author":"Adelino","year":"2021","journal-title":"Nat Commu."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2616","DOI":"10.1038\/s41467-021-22921-7","article-title":"Lying in wait: The resurgence of dengue virus after the Zika epidemic in Brazil","volume":"12","author":"Brito","year":"2021","journal-title":"Nat. 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