{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T10:32:28Z","timestamp":1768818748911,"version":"3.49.0"},"reference-count":57,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,8,26]],"date-time":"2023-08-26T00:00:00Z","timestamp":1693008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (ANPCyT, Argentina)","award":["PICT-2018-03137"],"award-info":[{"award-number":["PICT-2018-03137"]}]},{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (ANPCyT, Argentina)","award":["PICT-2019-03295"],"award-info":[{"award-number":["PICT-2019-03295"]}]},{"name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica (ANPCyT, Argentina)","award":["PIP-0218-GI"],"award-info":[{"award-number":["PIP-0218-GI"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET, Argentina)","award":["PICT-2018-03137"],"award-info":[{"award-number":["PICT-2018-03137"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET, Argentina)","award":["PICT-2019-03295"],"award-info":[{"award-number":["PICT-2019-03295"]}]},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET, Argentina)","award":["PIP-0218-GI"],"award-info":[{"award-number":["PIP-0218-GI"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The mammarenavirus Jun\u00edn (JUNV) is the causative agent of Argentine hemorrhagic fever, a severe disease of public health concern. The most abundant viral protein is the nucleoprotein (NP), a multifunctional, two-domain protein with the primary role as structural component of the viral nucleocapsids, used as template for viral polymerase RNA synthesis activities. Here, we report that the C-terminal domain (CTD) of the attenuated Candid#1 strain of the JUNV NP can be purified as a stable soluble form with a secondary structure in line with known NP structures from other mammarenaviruses. We show that the JUNV NP CTD interacts with the viral matrix protein Z in vitro, and that the full-length NP and Z interact with each other in cellulo, suggesting that the NP CTD is responsible for this interaction. This domain comprises an arrangement of four acidic residues and a histidine residue conserved in the active site of exoribonucleases belonging to the DEDDh family. We show that the JUNV NP CTD displays metal-ion-dependent nuclease activity against DNA and single- and double-stranded RNA, and that this activity is impaired by the mutation of a catalytic residue within the DEDDh motif. These results further support this activity, not previously observed in the JUNV NP, which could impact the mechanism of the cellular immune response modulation of this important pathogen.<\/jats:p>","DOI":"10.3390\/v15091818","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T02:23:25Z","timestamp":1693189405000},"page":"1818","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Nuclease Activity of the Jun\u00edn Virus Nucleoprotein C-Terminal Domain"],"prefix":"10.3390","volume":"15","author":[{"given":"Alicia Armella","family":"Sierra","sequence":"first","affiliation":[{"name":"Centro de Virolog\u00eda Humana y Animal (CEVHAN), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)-Universidad Abierta Interamericana, Buenos Aires C1287, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7965-0177","authenticated-orcid":false,"given":"Mar\u00eda Eugenia","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Humana y Animal (CEVHAN), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)-Universidad Abierta Interamericana, Buenos Aires C1287, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n","family":"Esperante","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, Instituto de Investigaciones Bioqu\u00edmicas de Buenos Aires (IIBBA) CONICET, Buenos Aires C1405, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Susana","family":"Borkosky","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, Instituto de Investigaciones Bioqu\u00edmicas de Buenos Aires (IIBBA) CONICET, Buenos Aires C1405, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1102-7108","authenticated-orcid":false,"given":"Giovanna L.","family":"Gallo","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Humana y Animal (CEVHAN), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)-Universidad Abierta Interamericana, Buenos Aires C1287, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5748-6863","authenticated-orcid":false,"given":"Gonzalo","family":"de Prat Gay","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n Instituto Leloir, Instituto de Investigaciones Bioqu\u00edmicas de Buenos Aires (IIBBA) CONICET, Buenos Aires C1405, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1932-1843","authenticated-orcid":false,"given":"Nora","family":"Lopez","sequence":"additional","affiliation":[{"name":"Centro de Virolog\u00eda Humana y Animal (CEVHAN), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)-Universidad Abierta Interamericana, Buenos Aires C1287, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1099\/jgv.0.001280","article-title":"ICTV Virus Taxonomy Profile: Arenaviridae","volume":"100","author":"Radoshitzky","year":"2019","journal-title":"J. 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