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Serological testing has been used to investigate dengue-infected patients, but specificity is affected by the co-circulation of ZIKA virus (ZIKV), which shares extensive antigen similarities. The goal of this study was the development of a specific dengue virus (DENV) IgG ELISA based on a multi-epitope NS1-based antigen for antibody detection. The multi-epitope protein (T-\u0394NS1), derived from a fragment of the NS1-protein of the four DENV serotypes, was expressed in Escherichia coli and purified via affinity chromatography. The antigenicity and specificity were evaluated with sera of mice infected with DENV-1\u20134 or ZIKV or after immunization with the recombinant \u0394NS1 proteins. The performance of the T-\u0394NS1-based IgG ELISA was also determined with human serum samples. The results demonstrate that the DENV T-\u0394NS1 was specifically recognized by the serum IgG of dengue-infected mice or humans but showed no or reduced reactivity with ZIKV-infected subjects. Based on the available set of clinical samples, the ELISA based on the DENV T-\u0394NS1 achieved 77.42% sensitivity and 88.57% specificity. The results indicate that the T-\u0394NS1 antigen is a promising candidate for the development of specific serological analysis.<\/jats:p>","DOI":"10.3390\/v15091936","type":"journal-article","created":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T06:36:45Z","timestamp":1694932605000},"page":"1936","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Multi-epitope Antigen for Specific Serological Detection of Dengue Viruses"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9860-224X","authenticated-orcid":false,"given":"Samuel Santos","family":"Pereira","sequence":"first","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Andreata-Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2162-9505","authenticated-orcid":false,"given":"Maria Fernanda","family":"de Castro-Amarante","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Institut Pasteur de S\u00e3o Paulo, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al\u00e9xia Adrianne","family":"Venceslau-Carvalho","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Institut Pasteur de S\u00e3o Paulo, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natiely Silva","family":"Sales","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0975-7187","authenticated-orcid":false,"given":"Mari\u00e2ngela de Oliveira","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5538-6025","authenticated-orcid":false,"given":"R\u00fabens Prince dos Santos","family":"Alves","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patr\u00edcia","family":"Jungmann","sequence":"additional","affiliation":[{"name":"General Pathology, Universidade de Pernambuco, Recife 50100-130, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4883-1693","authenticated-orcid":false,"given":"Lu\u00eds Carlos de Souza","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laboratory of Vaccine Development, Institute of Biomedical Sciences, Department of Microbiology, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Institut Pasteur de S\u00e3o Paulo, S\u00e3o Paulo 05508-020, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mousson, L., Zouache, K., Arias-Goeta, C., Raquin, V., Mavingui, P., and Failloux, A.B. 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