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Reverse genetics systems realize the manipulation of RNA virus genome and facilitate the development of new vaccines. Thus far, five reverse genetics approaches have been successfully applied to engineer the swine CoV genome: targeted RNA recombination, in vitro ligation, bacterial artificial chromosome-based ligation, vaccinia virus -based recombination, and yeast-based method. This review summarizes the advantages and limitations of these approaches; it also discusses the latest research progress in terms of their use for virus-related pathogenesis elucidation, vaccine candidate development, antiviral drug screening, and virus replication mechanism determination.<\/jats:p>","DOI":"10.3390\/v15102003","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T03:04:18Z","timestamp":1695783858000},"page":"2003","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Reverse Genetics Systems for Emerging and Re-Emerging Swine Coronaviruses and Applications"],"prefix":"10.3390","volume":"15","author":[{"given":"Hui","family":"Jiang","sequence":"first","affiliation":[{"name":"Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Department of Avian Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai 200241, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingbao","family":"Kong","sequence":"additional","affiliation":[{"name":"Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1318-7081","authenticated-orcid":false,"given":"Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8128-1697","authenticated-orcid":false,"given":"Qi","family":"Peng","sequence":"additional","affiliation":[{"name":"Nanchang Key Laboratory of Animal Virus and Genetic Engineering, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Institute of Pathogenic Microorganism, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China"},{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/3-540-26765-4_1","article-title":"Coronavirus genome structure and replication","volume":"Volume 287","author":"Brian","year":"2005","journal-title":"Coronavirus Replication and Reverse Genetics. 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