{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:06:35Z","timestamp":1784898395544,"version":"3.55.0"},"reference-count":59,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870144"],"award-info":[{"award-number":["31870144"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Previously, we identified a highly conserved, \u03b3-shaped RNA element (\u03b3RE) from satellite RNAs of cucumber mosaic virus (CMV), and we determined \u03b3RE to be structurally required for satRNA survival and the inhibition of CMV replication. It remains unknown how \u03b3RE biologically functions. In this work, pull-down assays were used to screen candidates of host factors from Nicotiana benthamiana plants using biotin-labeled \u03b3RE as bait. Nine host factors were found to interact specifically with \u03b3RE. Then, all of these host factors were down-regulated individually in N. benthamiana plants via tobacco rattle virus-induced gene silencing and tested with infection by GFP-expressing CMV (CMV-gfp) and the isolate T1 of satRNA (sat-T1). Out of nine candidates, three host factors, namely histone H3, GTPase Ran3, and eukaryotic translation initiation factor 4A, were extremely important for infection by CMV-gfp and sat-T1. Moreover, we found that cytosolic glyceraldehyde-3-phosphate dehydrogenase 2 contributed to the replication of CMV and sat-T1, but also negatively regulated CMV 2b activity. Collectively, our work provides essential clues for uncovering the mechanism by which satRNAs inhibit CMV replication.<\/jats:p>","DOI":"10.3390\/v15102039","type":"journal-article","created":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T16:58:09Z","timestamp":1696179489000},"page":"2039","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification of Host Factors Interacting with a \u03b3-Shaped RNA Element from a Plant Virus-Associated Satellite RNA"],"prefix":"10.3390","volume":"15","author":[{"given":"Mengjiao","family":"Li","sequence":"first","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobei","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaiyun","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyou","family":"Du","sequence":"additional","affiliation":[{"name":"College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.3390\/v1031325","article-title":"Satellite RNAs and Satellite Viruses of Plants","volume":"1","author":"Hu","year":"2009","journal-title":"Viruses"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1007\/s10327-014-0547-x","article-title":"Molecular biology of Cucumber mosaic virus and its satellite RNA","volume":"80","author":"Masuta","year":"2014","journal-title":"J. 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