{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T13:25:37Z","timestamp":1783689937033,"version":"3.55.0"},"reference-count":29,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Estonian Ministry of Education and Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Hepatitis E virus (HEV) is now considered the most common cause of acute hepatitis worldwide. There are no published data about the prevalence of antibodies to HEV and RNA in donor sera in Estonia, and this precludes planning measures for preventing HEV proliferation through blood transfusion services. Here, were report data from an analysis of 1002 sera on the prevalence of anti-HEV IgG and IgM and the viral RNA. The antibodies were found in 48 donor sera (4.8%); of these, 40 (4%) harbored anti-HEV IgG, 15 (1.5%) contained anti-HEV IgM, and 7 donors had anti-HEV antibodies of both classes simultaneously. HEV RNA was not detected in any blood serum. Statistical associations of infection risk factors (gender, age, travel in the last six months, contact with pigs and\/or wild boars in the last six months, consumption of thermally unprocessed\/raw pork or boar meat, raw\/unfiltered tap water or water from natural sources, unpasteurized farm dairy products, and unwashed berries and\/or vegetables) were assessed. None of the listed factors were found to be associated with a higher or lower risk of anti-HEV antibody presence. At the same time, an increasing share of anti-HEV IgG carriers with age was found. The absence of HEV RNA in the analyzed donor plasma samples proves that HEV acute infection prevalence in Estonia does not exceed the average level of European countries. There is no urgent necessity to enter a requirement for a total screening of blood plasma for HEV RNA prevalence in Estonia.<\/jats:p>","DOI":"10.3390\/v15102118","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T02:54:43Z","timestamp":1697684083000},"page":"2118","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of Prevalence of Hepatitis E Clinical Markers among Donors in Estonia"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7285-5038","authenticated-orcid":false,"given":"Tatiana","family":"Kuznetsova","sequence":"first","affiliation":[{"name":"Department of Infectious Disease Research, National Institute for Health Development, 11619 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Moor","sequence":"additional","affiliation":[{"name":"North Estonia Medical Centre\u2019s Blood Center, North Estonia Medical Centre Foundation, 13419 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0037-5247","authenticated-orcid":false,"given":"Gulara","family":"Khanirzayeva","sequence":"additional","affiliation":[{"name":"North Estonia Medical Centre\u2019s Blood Center, North Estonia Medical Centre Foundation, 13419 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6496-9687","authenticated-orcid":false,"given":"Julia","family":"Geller","sequence":"additional","affiliation":[{"name":"Department of Infectious Disease Research, National Institute for Health Development, 11619 Tallinn, Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,19]]},"reference":[{"key":"ref_1","first-page":"177","article-title":"E-viirushepatiidi viiruse antikehade esinemine Eesti inimestel. Katseuuring","volume":"91","author":"Tefanova","year":"2012","journal-title":"Eest. Arst"},{"key":"ref_2","first-page":"587","article-title":"\u00c4ge E-viirushepatiit. Haigusjuhu kirjeldus","volume":"92","author":"Proshkina","year":"2013","journal-title":"Eest. Arst"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"17086","DOI":"10.1038\/nrdp.2017.86","article-title":"Hepatitis E virus infection","volume":"3","author":"Kamar","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e158","DOI":"10.1017\/S0950268820001429","article-title":"Hepatitis E virus and blood transfusion safety","volume":"148","author":"Bi","year":"2020","journal-title":"Epidemiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1007\/s12560-023-09561-4","article-title":"Detection of Hepatitis A RNA, Hepatitis E RNA, Human Adenovirus F DNA, and Norovirus RNA in Fresh and Frozen Berry Products at Point of Retail in Ireland","volume":"15","author":"Bennett","year":"2023","journal-title":"Food Environ. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/s12560-015-9210-8","article-title":"Hepatitis E Virus in Domestic Pigs, Wild Boars, Pig Farm Workers, and Hunters in Estonia","volume":"7","author":"Ivanova","year":"2015","journal-title":"Food Environ. Virol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1089\/vbz.2017.2122","article-title":"Prevalence of Antibodies against Hepatitis E Virus in Veterinarians in Estonia","volume":"17","author":"Lassen","year":"2017","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jcv.2018.04.011","article-title":"Hepatitis E virus infection in different groups of Estonian patients and people who inject drugs","volume":"104","author":"Kuznetsova","year":"2018","journal-title":"J. Clin. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1159\/000499121","article-title":"Blood Donor Screening for Hepatitis E Virus in the European Union","volume":"46","author":"Boland","year":"2019","journal-title":"Transfus. Med. Hemother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1423-0410.2011.01583.x","article-title":"Occurrence of hepatitis E virus RNA in plasma donations from Sweden, Germany and the United States","volume":"103","author":"Baylis","year":"2012","journal-title":"Vox Sang."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Matto, J., Putkuri, N., Rimhanen-Finne, R., Laurila, P., Clancy, J., Ihalainen, J., and Ekblom-Kullberg, S. (2023). Hepatitis E Virus in Finland: Epidemiology and Risk in Blood Donors and in the General Population. Pathogens, 12.","DOI":"10.3390\/pathogens12030484"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1111\/trf.14531","article-title":"Molecular and serological infection marker screening in blood donors indicates high endemicity of hepatitis E virus in Poland","volume":"58","author":"Grabarczyk","year":"2018","journal-title":"Transfusion"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.3748\/wjg.v25.i25.3168","article-title":"Epidemiology of hepatitis E in South-East Europe in the \u201cOne Health\u201d concept","volume":"25","author":"Mrzljak","year":"2019","journal-title":"World J. Gastroenterol."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Mikhailov, M.I., Karlsen, A.A., Potemkin, I.A., Isaeva, O.V., Kichatova, V.S., Malinnikova, E.Y., Asadi Mobarkhan, F.A., Mullin, E.V., Lopatukhina, M.A., and Manuylov, V.A. (2022). Geographic and Temporal Variability of Hepatitis E Virus Circulation in the Russian Federation. Viruses, 15.","DOI":"10.20944\/preprints202211.0286.v1"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Capai, L., Masse, S., Gallian, P., Souty, C., Isnard, C., Blanchon, T., Peres, B., de Lamballerie, X., Charrel, R., and Falchi, A. (2019). Seroprevalence Study of Anti-HEV IgG among Different Adult Populations in Corsica, France, 2019. Microorganisms, 7.","DOI":"10.3390\/microorganisms7100460"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1007\/s00430-017-0503-4","article-title":"Performance of Hepatitis E Virus (HEV)-antibody tests: A comparative analysis based on samples from individuals with direct contact to domestic pigs or wild boar in Germany","volume":"206","author":"Sommerkorn","year":"2017","journal-title":"Med. Microbiol. Immunol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"30561","DOI":"10.2807\/1560-7917.ES.2017.22.26.30561","article-title":"Hepatitis E virus infection in Europe: Surveillance and descriptive epidemiology of confirmed cases, 2005 to 2015","volume":"22","author":"Aspinall","year":"2017","journal-title":"Eurosurveillance"},{"key":"ref_18","unstructured":"World Health Organization (WHO) (2022). Global Health Sector Strategies on, Respectively, HIV, Viral Hepatitis and Sexually Transmitted Infections for the Period 2022\u20132030, WHO. Available online: https:\/\/iris.who.int\/bitstream\/handle\/10665\/360348\/9789240053779-eng.pdf?sequence=1."},{"key":"ref_19","first-page":"70","article-title":"Prevalence of HEV infection in acute non-ABC hepatitis and prognostic role of extrahepatic manifestations","volume":"31","author":"Picchi","year":"2022","journal-title":"Infez. Med."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Grigas, J., Montoya, M., Simkute, E., Buitkus, M., Zagrabskaite, R., Pautienius, A., Razukevicius, D., Jonaitis, L.V., Kiudelis, G., and Skieceviciene, J. (2021). Molecular Characterization and Seroprevalence of Hepatitis E Virus in Inflammatory Bowel Disease Patients and Solid Organ Transplant Recipients. Viruses, 13.","DOI":"10.3390\/v13040670"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1080\/00365540500321470","article-title":"Unexpected high prevalence of IgG-antibodies to hepatitis E virus in Swedish pig farmers and controls","volume":"38","author":"Olsen","year":"2006","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1128\/JCM.02343-15","article-title":"Diagnostic Performance of Five Assays for Anti-Hepatitis E Virus IgG and IgM in a Large Cohort Study","volume":"54","author":"Norder","year":"2016","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/trf.12121","article-title":"Seroprevalence and incidence of hepatitis E virus infection in German blood donors","volume":"54","author":"Juhl","year":"2014","journal-title":"Transfusion"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.3201\/eid1810.111756","article-title":"Hepatitis E virus seroprevalence among adults, Germany","volume":"18","author":"Faber","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"20550","DOI":"10.2807\/1560-7917.ES2013.18.31.20550","article-title":"Silent hepatitis E virus infection in Dutch blood donors, 2011 to 2012","volume":"18","author":"Slot","year":"2013","journal-title":"Eurosurveillance"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.ijid.2017.05.014","article-title":"Hepatitis E virus IgG seroprevalence in HIV patients and blood donors, west-central Poland","volume":"61","author":"Bura","year":"2017","journal-title":"Int. J. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Hartl, J., Otto, B., Madden, R.G., Webb, G., Woolson, K.L., Kriston, L., Vettorazzi, E., Lohse, A.W., Dalton, H.R., and Pischke, S. (2016). Hepatitis E Seroprevalence in Europe: A Meta-Analysis. Viruses, 8.","DOI":"10.3390\/v8080211"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Wolski, A., Pischke, S., Ozga, A.K., Addo, M.M., and Horvatits, T. (2023). Higher Risk of HEV Transmission and Exposure among Blood Donors in Europe and Asia in Comparison to North America: A Meta-Analysis. Pathogens, 12.","DOI":"10.3390\/pathogens12030425"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1111\/trf.12929","article-title":"Seroprevalence of hepatitis E virus (HEV) and detection of HEV RNA with a transcription-mediated amplification assay in blood donors from Catalonia (Spain)","volume":"55","author":"Sauleda","year":"2015","journal-title":"Transfusion"}],"container-title":["Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/10\/2118\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:09:34Z","timestamp":1760130574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1999-4915\/15\/10\/2118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,19]]},"references-count":29,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["v15102118"],"URL":"https:\/\/doi.org\/10.3390\/v15102118","relation":{},"ISSN":["1999-4915"],"issn-type":[{"value":"1999-4915","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,19]]}}}