{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T14:39:33Z","timestamp":1791729573235,"version":"4.3.4"},"reference-count":76,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004809","name":"Rede Corona-\u00f4mica BR MCTI\/FINEP","doi-asserted-by":"publisher","award":["01.20.0029.000462\/20"],"award-info":[{"award-number":["01.20.0029.000462\/20"]}],"id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Rede Corona-\u00f4mica BR MCTI\/FINEP","doi-asserted-by":"publisher","award":["443215\/2019-7"],"award-info":[{"award-number":["443215\/2019-7"]}],"id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Rede Corona-\u00f4mica BR MCTI\/FINEP","doi-asserted-by":"publisher","award":["404096\/2020-4"],"award-info":[{"award-number":["404096\/2020-4"]}],"id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["01.20.0029.000462\/20"],"award-info":[{"award-number":["01.20.0029.000462\/20"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["443215\/2019-7"],"award-info":[{"award-number":["443215\/2019-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"National Council for Scientific and Technological Development","doi-asserted-by":"publisher","award":["404096\/2020-4"],"award-info":[{"award-number":["404096\/2020-4"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>In Brazil, the state of Tocantins, located in north-central Brazil, has experienced a significant number of cases of arboviral disease, particularly Dengue virus (DENV). This study aimed to deepen the knowledge on DENV circulation within that state by conducting full genome sequencing of viral genomes recovered from 61 patients between June 2021 and July 2022. There were a total of 8807 and 20,692 cases in 2021 and 2022, respectively, as reported by the state\u2019s Secretary of Health. Nucleotide sequencing confirmed the circulation of DENV serotype 1, genotype V and DENV serotype 2, genotype III in the State. Younger age groups (4 to 43 years old) were mostly affected; however, no significant differences were detected regarding the gender distribution of cases in humans. Phylogenetic analysis revealed that the circulating viruses belong to DENV-1 genotype V American and DENV-2 genotype III Southeast Asian\/American. The Bayesian analysis of DENV-1 genotype V genomes sequenced here are closely related to genomes previously sequenced in the state of S\u00e3o Paulo. Regarding the DENV-2 genotype III genomes, these clustered in a distinct, well-supported subclade, along with previously reported isolates from the states of Goi\u00e1s and S\u00e3o Paulo. The findings reported here suggest that multiple introductions of these genotypes occurred in the Tocantins state. This observation highlights the importance of major population centers in Brazil on virus dispersion, such as those observed in other Latin American and North American countries. In the SNP analysis, DENV-1 displayed 122 distinct missense mutations, while DENV-2 had 44, with significant mutations predominantly occurring in the envelope and NS5 proteins. The analyses performed here highlight the concomitant circulation of distinct DENV-1 and -2 genotypes in some Brazilian states, underscoring the dynamic evolution of DENV and the relevance of surveillance efforts in supporting public health policies.<\/jats:p>","DOI":"10.3390\/v15112136","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T06:29:01Z","timestamp":1698128941000},"page":"2136","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Circulation of Dengue Virus Serotype 1 Genotype V and Dengue Virus Serotype 2 Genotype III in Tocantins State, Northern Brazil, 2021\u20132022"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-4771","authenticated-orcid":false,"given":"Ueric Jos\u00e9 Borges de","family":"Souza","sequence":"first","affiliation":[{"name":"Bioinformatics and Biotechnology Laboratory, Campus of Gurupi, Federal University of Tocantins, Gurupi 77410-570, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2133-8858","authenticated-orcid":false,"given":"Ygor da Silva Miranda","family":"Macedo","sequence":"additional","affiliation":[{"name":"Bioinformatics and Biotechnology Laboratory, Campus of Gurupi, Federal University of Tocantins, Gurupi 77410-570, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3388-890X","authenticated-orcid":false,"given":"Ra\u00edssa Nunes dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Bioinformatics and Biotechnology Laboratory, Campus of Gurupi, Federal University of Tocantins, Gurupi 77410-570, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0559-3082","authenticated-orcid":false,"given":"Franciano Dias Pereira","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Central Public Health Laboratory of the State of Tocantins, Palmas 77054-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jucim\u00e1ria Dantas","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Central Public Health Laboratory of the State of Tocantins, Palmas 77054-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeni Evgeniev","family":"Gabev","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, Medical University of Sofia, 1431 Sofia, Bulgaria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cl\u00e1udia","family":"Franco","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Department of Microbiology, Immunology, and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre 90050-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-7661","authenticated-orcid":false,"given":"Paulo Michel","family":"Roehe","sequence":"additional","affiliation":[{"name":"Virology Laboratory, Department of Microbiology, Immunology, and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre 90050-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5804-7045","authenticated-orcid":false,"given":"Fernando Rosado","family":"Spilki","sequence":"additional","affiliation":[{"name":"Molecular Microbiology Laboratory, Feevale University, Novo Hamburgo 93525-075, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5948-472X","authenticated-orcid":false,"given":"Fabr\u00edcio Souza","family":"Campos","sequence":"additional","affiliation":[{"name":"Bioinformatics and Biotechnology Laboratory, Campus of Gurupi, Federal University of Tocantins, Gurupi 77410-570, Brazil"},{"name":"Virology Laboratory, Department of Microbiology, Immunology, and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre 90050-170, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"key":"ref_1","first-page":"1","article-title":"Dengue, its history, epidemiology, mechanism of transmission, etiology, clinical manifestations, immunity and prevention","volume":"29","author":"Siler","year":"1926","journal-title":"Philipp J. 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