{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T13:54:14Z","timestamp":1790430854744,"version":"4.1.0"},"reference-count":41,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T00:00:00Z","timestamp":1700438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["5R01CA240983-05"],"award-info":[{"award-number":["5R01CA240983-05"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["5R01CA211096-05"],"award-info":[{"award-number":["5R01CA211096-05"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P19-00559 B101"],"award-info":[{"award-number":["P19-00559 B101"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Centene Corporation contract for the Washington University-Centene ARCH Personalized Medicine Initiative","award":["5R01CA240983-05"],"award-info":[{"award-number":["5R01CA240983-05"]}]},{"name":"Centene Corporation contract for the Washington University-Centene ARCH Personalized Medicine Initiative","award":["5R01CA211096-05"],"award-info":[{"award-number":["5R01CA211096-05"]}]},{"name":"Centene Corporation contract for the Washington University-Centene ARCH Personalized Medicine Initiative","award":["P19-00559 B101"],"award-info":[{"award-number":["P19-00559 B101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>mRNA vaccines have attracted widespread research attention with clear advantages in terms of molecular flexibility, rapid development, and potential for personalization. However, current mRNA vaccine platforms have not been optimized for induction of CD4\/CD8 T cell responses. In addition, the mucosal administration of mRNA based on lipid nanoparticle technology faces challenges in clinical translation. In contrast, adenovirus-based vaccines induce strong T cell responses and have been approved for intranasal delivery. To leverage the inherent strengths of both the mRNA and adenovirus platforms, we developed a novel modular adenoviral mRNA delivery platform based on Tag\/Catcher bioconjugation. Specifically, we engineered adenoviral vectors integrating Tag\/Catcher proteins at specific locales on the Ad capsid proteins, allowing us to anchor mRNA to the surface of engineered Ad viruses. In proof-of-concept studies, the Ad-mRNA platform successfully mediated mRNA delivery and could be optimized via the highly flexible modular design of both the Ad-mRNA and protein bioconjugation systems.<\/jats:p>","DOI":"10.3390\/v15112277","type":"journal-article","created":{"date-parts":[[2023,11,20]],"date-time":"2023-11-20T10:32:32Z","timestamp":1700476352000},"page":"2277","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Engineering a Novel Modular Adenoviral mRNA Delivery Platform Based on Tag\/Catcher Bioconjugation"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-9240-7736","authenticated-orcid":false,"given":"Kexin","family":"Geng","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Biologic Therapeutics Center, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"Department of Biomedical Engineering, McKelvey School of Engineering, Washington University in Saint Louis, St. Louis, MO 63130-4899, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Rice-Boucher","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Biologic Therapeutics Center, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"Department of Biomedical Engineering, McKelvey School of Engineering, Washington University in Saint Louis, St. Louis, MO 63130-4899, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena A.","family":"Kashentseva","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Biologic Therapeutics Center, Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9206-1975","authenticated-orcid":false,"given":"Igor P.","family":"Dmitriev","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Biologic Therapeutics Center, Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi Hong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Biologic Therapeutics Center, Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. Peter","family":"Goedegebuure","sequence":"additional","affiliation":[{"name":"Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine, St. Louis, MO 63110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William E.","family":"Gillanders","sequence":"additional","affiliation":[{"name":"Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA"},{"name":"Alvin J. 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