{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:24:45Z","timestamp":1789431885963,"version":"build-2803163510"},"reference-count":50,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"InfectioP\u00f4le Sud Mediterran\u00e9e 2020","award":["AO-IHE-2020-3"],"award-info":[{"award-number":["AO-IHE-2020-3"]}]},{"name":"University Hospital of N\u00eemes","award":["AO-IHE-2020-3"],"award-info":[{"award-number":["AO-IHE-2020-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Staphylococcus sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems. Phage therapy is an alternative treatment to antibiotics, overcoming the issue of antibiotic resistance. In this study, six phages (SAVM01 to SAVM06) were isolated from effluents and were used against a panel of staphylococcal clinical samples isolated from DFUs. A genomic analysis revealed that the phages belonged to the Herelleviridae family, with sequences similar to those of the Kayvirus genus. No lysogeny-associated genes, known virulence or drug resistance genes were identified in the phage genomes. The phages displayed a strong lytic and antibiofilm activity against DFU clinical isolates, as well as against opportunistic pathogenic coagulase-negative staphylococci. The results presented here suggest that these phages could be effective biocontrol agents against staphylococcal clinical isolates from DFUs.<\/jats:p>","DOI":"10.3390\/v15122287","type":"journal-article","created":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T08:47:43Z","timestamp":1700642863000},"page":"2287","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2128-8010","authenticated-orcid":false,"given":"Lucile","family":"Plumet","sequence":"first","affiliation":[{"name":"VBIC, INSERM U1047, University of Montpellier, 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8074-1794","authenticated-orcid":false,"given":"Madjid","family":"Morsli","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, University of Montpellier, CHU N\u00eemes, 30908 N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7868-8368","authenticated-orcid":false,"given":"Nour","family":"Ahmad-Mansour","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, University of Montpellier, 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Clavijo-Coppens","sequence":"additional","affiliation":[{"name":"Greenphage, Cap Alpha, 34830 Clapiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Berry","sequence":"additional","affiliation":[{"name":"Laboratory of Pathogen and Host Immunity, CNRS UMR5294, University of Montpellier, 34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6463-9386","authenticated-orcid":false,"given":"Albert","family":"Sotto","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, Department of Infectious Diseases, University of de Montpellier, CHU N\u00eemes, 30908 N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9484-0304","authenticated-orcid":false,"given":"Jean-Philippe","family":"Lavigne","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, University of Montpellier, CHU N\u00eemes, 30908 N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Costechareyre","sequence":"additional","affiliation":[{"name":"Greenphage, Cap Alpha, 34830 Clapiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6573-3411","authenticated-orcid":false,"given":"Virginie","family":"Molle","sequence":"additional","affiliation":[{"name":"VBIC, INSERM U1047, University of Montpellier, 34095 Montpellier, France"},{"name":"VBIC, INSERM U1047, Department of Microbiology and Hospital Hygiene, University of Montpellier, CHU N\u00eemes, 30908 N\u00eemes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e3280","DOI":"10.1002\/dmrr.3280","article-title":"Guidelines on the Diagnosis and Treatment of Foot Infection in Persons with Diabetes (IWGDF 2019 Update)","volume":"36","author":"Lipsky","year":"2020","journal-title":"Diabetes\/Metab. 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