{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T10:54:36Z","timestamp":1773744876284,"version":"3.50.1"},"reference-count":42,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordination for the Improvement of Higher Education Personnel, Brazilian National Council for Scientific and Technological Development","award":["88882.332025\/2019-01"],"award-info":[{"award-number":["88882.332025\/2019-01"]}]},{"name":"Young Scientist of our state FAPERJ (protocol E03\/2017)","award":["88882.332025\/2019-01"],"award-info":[{"award-number":["88882.332025\/2019-01"]}]},{"name":"IBRO Young Scientist Award","award":["88882.332025\/2019-01"],"award-info":[{"award-number":["88882.332025\/2019-01"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>Congenital Zika syndrome (CZS) is a set of birth defects caused by Zika virus (ZIKV) infection during pregnancy. Microcephaly is its main feature, but other brain abnormalities are found in CZS patients, such as ventriculomegaly, brain calcifications, and dysgenesis of the corpus callosum. Many studies have focused on microcephaly, but it remains unknown how ZIKV infection leads to callosal malformation. To tackle this issue, we infected mouse embryos in utero with a Brazilian ZIKV isolate and found that they were born with a reduction in callosal area and density of callosal neurons. ZIKV infection also causes a density reduction in PH3+ cells, intermediate progenitor cells, and SATB2+ neurons. Moreover, axonal tracing revealed that callosal axons are reduced and misrouted. Also, ZIKV-infected cultures show a reduction in callosal axon length. GFAP labeling showed that an in utero infection compromises glial cells responsible for midline axon guidance. In sum, we showed that ZIKV infection impairs critical steps of corpus callosum formation by disrupting not only neurogenesis, but also axon guidance and growth across the midline.<\/jats:p>","DOI":"10.3390\/v15122336","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T11:43:16Z","timestamp":1701171796000},"page":"2336","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Congenital Zika Virus Infection Impairs Corpus Callosum Development"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7052-2643","authenticated-orcid":false,"given":"Raissa Rilo","family":"Christoff","sequence":"first","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jefferson H.","family":"Quintanilha","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2326-9494","authenticated-orcid":false,"given":"Raiane Oliveira","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"},{"name":"Human Genome and Stem Cell Research Center, Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-090, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica C. C. G.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Menezes","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna","family":"Val\u00e9rio-Gomes","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"},{"name":"Institute of Medical Biochemistry Leopoldo de Meis, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiza M.","family":"Higa","sequence":"additional","affiliation":[{"name":"Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6235-8807","authenticated-orcid":false,"given":"\u00c1tila D.","family":"Rossi","sequence":"additional","affiliation":[{"name":"Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3360-2740","authenticated-orcid":false,"given":"Maria","family":"Bellio","sequence":"additional","affiliation":[{"name":"Institute of Microbiology Paulo de G\u00f3es, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0570-750X","authenticated-orcid":false,"given":"Amilcar","family":"Tanuri","sequence":"additional","affiliation":[{"name":"Department of Genetics, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Lent","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"},{"name":"Institute D\u2019Or for Research and Education, Rio de Janeiro 2281-100, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia Pestana","family":"Garcez","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro 21941-590, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1056\/NEJMoa1600651","article-title":"Zika virus associated with microcephaly","volume":"374","author":"Mlakar","year":"2016","journal-title":"N. 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