{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:21:10Z","timestamp":1783948870218,"version":"3.55.0"},"reference-count":84,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CR-UK","award":["C17183\/A23303"],"award-info":[{"award-number":["C17183\/A23303"]}]},{"name":"CR-UK","award":["G2021035005L"],"award-info":[{"award-number":["G2021035005L"]}]},{"name":"High-End Foreign Experts Program of the Ministry of Science and Technology of China","award":["C17183\/A23303"],"award-info":[{"award-number":["C17183\/A23303"]}]},{"name":"High-End Foreign Experts Program of the Ministry of Science and Technology of China","award":["G2021035005L"],"award-info":[{"award-number":["G2021035005L"]}]},{"name":"University of Birmingham JustGiving scheme","award":["C17183\/A23303"],"award-info":[{"award-number":["C17183\/A23303"]}]},{"name":"University of Birmingham JustGiving scheme","award":["G2021035005L"],"award-info":[{"award-number":["G2021035005L"]}]},{"name":"Freie und Hansestadt Hamburg","award":["C17183\/A23303"],"award-info":[{"award-number":["C17183\/A23303"]}]},{"name":"Freie und Hansestadt Hamburg","award":["G2021035005L"],"award-info":[{"award-number":["G2021035005L"]}]},{"name":"German Bundesministerium f\u00fcr Gesundheit (Federal Ministry of Health)","award":["C17183\/A23303"],"award-info":[{"award-number":["C17183\/A23303"]}]},{"name":"German Bundesministerium f\u00fcr Gesundheit (Federal Ministry of Health)","award":["G2021035005L"],"award-info":[{"award-number":["G2021035005L"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Viruses"],"abstract":"<jats:p>The adenovirus C5 E1B-55K protein is crucial for viral replication and is expressed early during infection. It can interact with E4orf6 to form a complex that functions as a ubiquitin E3 ligase. This complex targets specific cellular proteins and marks them for ubiquitination and, predominantly, subsequent proteasomal degradation. E1B-55K interacts with various proteins, with p53 being the most extensively studied, although identifying binding sites has been challenging. To explain the diverse range of proteins associated with E1B-55K, we hypothesized that other binding partners might recognize the simple p53 binding motif (xWxxxPx). In silico analyses showed that many known E1B-55K binding proteins possess this amino acid sequence; therefore, we investigated whether other xWxxxPx-containing proteins also bind to E1B-55K. Our findings revealed that many cellular proteins, including ATR, CHK1, USP9, and USP34, co-immunoprecipitate with E1B-55K. During adenovirus infection, several well-characterized E1B-55K binding proteins and newly identified interactors, including CSB, CHK1, and USP9, are degraded in a cullin-dependent manner. Notably, certain binding proteins, such as ATR and USP34, remain undegraded during infection. Structural predictions indicate no conservation of structure around the proposed binding motif, suggesting that the interaction relies on the correct arrangement of tryptophan and proline residues.<\/jats:p>","DOI":"10.3390\/v15122356","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T09:37:54Z","timestamp":1701337074000},"page":"2356","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Identification of Adenovirus E1B-55K Interaction Partners through a Common Binding Motif"],"prefix":"10.3390","volume":"15","author":[{"given":"Nafiseh","family":"Chalabi Hagkarim","sequence":"first","affiliation":[{"name":"Institute for Cancer and Genomic Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wing-Hang","family":"Ip","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Virology, Department of Viral Transformation, 20251 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0698-5395","authenticated-orcid":false,"given":"Luca D.","family":"Bertzbach","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Virology, Department of Viral Transformation, 20251 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tareq","family":"Abualfaraj","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Taibah University, P.O. Box 344, Madinah 41477, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7392-8588","authenticated-orcid":false,"given":"Thomas","family":"Dobner","sequence":"additional","affiliation":[{"name":"Leibniz Institute of Virology, Department of Viral Transformation, 20251 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3880-607X","authenticated-orcid":false,"given":"David P.","family":"Molloy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, School of Basic Medical Science, Chongqing Medical University, Chongqing 400016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0960-3241","authenticated-orcid":false,"given":"Grant S.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Institute for Cancer and Genomic Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-3267","authenticated-orcid":false,"given":"Roger J.","family":"Grand","sequence":"additional","affiliation":[{"name":"Institute for Cancer and Genomic Sciences, The Medical School, University of Birmingham, Birmingham B15 2TT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"001721","DOI":"10.1099\/jgv.0.001721","article-title":"ICTV Virus Taxonomy Profile: Adenoviridae 2022","volume":"103","author":"Benko","year":"2022","journal-title":"J. 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